Evaluation of uncertainty in the determination of acid scarlet GR in orange juice by high performance liquid chromatography

Authors

YUAN Yuan, Changsha Institute for Food and Drug Control, Changsha, Hunan 410036 , China ；National Alcohol Products Quality Inspection and Testing Center 〔Hunan〕, Changsha, Hunan 410036 , China
ZENG Hui, Changsha Institute for Food and Drug Control, Changsha, Hunan 410036 , China ；National Alcohol Products Quality Inspection and Testing Center 〔Hunan〕, Changsha, Hunan 410036 , China
XIAO Yan, Hunan International Travel Heal Thcare Center 〔Portalclinic of Changsha Customs〕, Changsha, Hunan 410011 , China
YANG Li-xia, Changsha Institute for Food and Drug Control, Changsha, Hunan 410036 , China ；National Alcohol Products Quality Inspection and Testing Center 〔Hunan〕, Changsha, Hunan 410036 , China
CUI Xiao-jiao, Changsha Institute for Food and Drug Control, Changsha, Hunan 410036 , China ；National Alcohol Products Quality Inspection and Testing Center 〔Hunan〕, Changsha, Hunan 410036 , China
YANG Yun, Changsha Institute for Food and Drug Control, Changsha, Hunan 410036 , China ；National Alcohol Products Quality Inspection and Testing Center 〔Hunan〕, Changsha, Hunan 410036 , China
WU Hou-lu, Changsha Institute for Food and Drug Control, Changsha, Hunan 410036 , China ；National Alcohol Products Quality Inspection and Testing Center 〔Hunan〕, Changsha, Hunan 410036 , China

Abstract

Objective:The uncertainty of the determination of acid scarlet GR in orange juice by high performance liquid chromatography (HPLC) was evaluated. The source of uncertainty and the key factors affecting the test results were analyzed, so as to improve the accuracy of the test results.Methods:The mathematical model of uncertainty of acid scarlet GR content in orange juice was established. The factors that may introduce uncertainty in the detection process were analyzed, and the uncertainty components were evaluated.Results:The content of acid scarlet GR in self-made positive orange juice was 1.46 mg/kg, the expanded uncertainty was 0.11 mg/kg (including factork=2), and the determination result was expressed as:X=(1.46±0.11) mg/kg(k=2).Conclusion:This uncertainty mainly comes from the uncertainty introduced by the purity of reference materials, the linear fitting of standard curves and the analysis of high performance liquid chromatography.

Publication Date

9-2-2022

First Page

85

Last Page

89

DOI

10.13652/j.spjx.1003.5788.2022.80131

Recommended Citation

Yuan, YUAN; Hui, ZENG; Yan, XIAO; Li-xia, YANG; Xiao-jiao, CUI; Yun, YANG; and Hou-lu, WU (2022) "Evaluation of uncertainty in the determination of acid scarlet GR in orange juice by high performance liquid chromatography," Food and Machinery: Vol. 38: Iss. 6, Article 16.
DOI: 10.13652/j.spjx.1003.5788.2022.80131
Available at: https://www.ifoodmm.cn/journal/vol38/iss6/16

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