Objective: This study aimed to evaluate the uncertainty of determination by high performance liquid chromatography (HPLC) for the content of adevantame, a synthetic sweetener illegally added in syrup drugs. Methods: The sample is diluted with solvent, and the content of adevantame in the sample was determined by HPLC. Through the analysis of the factors affecting the uncertainty, such as the preparation of the reference solution, curve fitting, sample weighing, sample volumetric determination and instrument precision, the uncertainty was synthesized of each component, and the extended uncertainty is calculated. Results: The expanded uncertainty is 1.8 μg/mL. The influence factors from large to small was as follows: repeated sample determination, standard curve fitting, standard solution preparation, sample solution preparation. Conclusion: The established uncertainty evaluation method is accurate and reliable, and truly reflects the source and magnitude of uncertainty.

Publication Date


First Page


Last Page





[1] 梁莹,庞振国,崔炳群,等.无糖食品甜味剂的应用及其安全性[J].现代食品科技,2009,25(8):964-966.LIANG Y,PANG Z G,CUI B Q,et al.Application status and safety of sweeteners in sugar-free foods[J].Modern Food Science and Technology,2009,25(8):964-966.
[2] 冯海静.人工合成食品甜味剂及其检测技术的研究进展[J].现代食品,2017,11(22):24-26.FENG H J.Research progress of synthetic food sweeteners and its detection technology[J].Modern Food,2017,11(22):24-26.
[3] 赵辉,王鑫璇.人造甜味剂与人体健康风险[J].食品科学技术学报,2018,36(6):15-20.ZHAO H,WANG X X.Human health risks of artificial sweeteners[J].Journal of Food Science and Technology,2018,36(6):15-20.
[4] 上官海燕,宋亚青,朱妙飞,等.过量摄入甜味剂会危害儿童身心健康[J].中国食品,2020,49(22):104-107.SHANGGUAN H Y,SONG Y Q,ZHU M F,et al.Excessive intake of sweeteners can harm children's physical and mental health[J].China Food,2020,49(22):104-107.
[5] 沈燕飞,朱仙娜,姚澄,等.阀切换离子色谱技术同时检测饮料中的糖类、甜味剂和防腐剂[J].食品科学,2018,39(16):311-316.SHENG Y F,ZHU X N,YAO C,et al.Simultaneous detection of sugars,sweeteners and preservatives in beverages by valve switching ion chromatography[J].Food Science,2018,39(16):311-316.
[6] HASHEMI M,HABIBI A,JAHANSHAHI N.Determination of cyclamate in artificial sweeteners and beverages using headspace singledrop microextraction and gas chromatography flame-ionisation detection[J].Food Chemistry,2011,124(3):1 258-1 263.
[7] LORENZO R A,PENA M T,FERNANDEZ P,et al.Artificial sweeteners in beverages by ultra performance liquid chromatography with photodiode array and liquid chromatography tandem mass spectrometry[J].Food Control,2015,47:43-52.
[8] SAKAI H,YAMASHITA A,TAMURA M,et al.Simultaneous determination of sweeteners in beverages by LC-MS/MS[J].Food Addit Contam Part A Chem Anal Control Expo Risk Assess,2015,32(6):808-816.
[9] KUBICA P,NAMIESNIK J,WASIK A.Determination of eight artificial sweeteners and common Stevia rebaudiana glycosides in nonalcoholic and alcoholic beverages by reversed-phase liquid chromatography coupled with tandem mass spectrometry[J].Analytical and Bioanalytical Chemistry,2015,407(5):1 505-1 512.
[10] 国家市场监督管理总局.食品中爱德万甜的测定:BJS 202201[S/OL].(2022-02-11)[2022-04-15].https://www.samr.gov.cn/spcjs/bcjyff/202202/P020220211508275745998.pdf.State Administration for Market Regulation.Determination of edevantame in food:BJS 202201[S/OL].(2022-02-11)[2022-04-15].https://www.samr.gov.cn/spcjs/bcjyff/202202/P02022021150827 5745998.pdf.
[11] 李薇,金薇,乐健,等.梯度洗脱HPLC法测定盐酸氨溴索口服溶液中甜味剂含量[J].药物分析杂志,2012,32(12):2 208-2 212.LI W,JIN W,YUE J,et al.Gradient HPLC determination of sweeteners in ambroxol hydrochloride oral solution[J].Chin J Pharm Anal,2012,32(12):2 208-2 212.
[12] 吴镁春,吴崇乐.超高效液相色谱法检测两种口服液中糖精钠含量[J].怀化学院学报,2018,37(11):74-76.WU M C,WU C L.Determination of saccharin sodium in two oral liquids by ultra high performance liquid chromatography[J].J of Huaihua University,2018,37(11):74-76.
[13] 国家质量监督检验检疫总局.测量不确定度评定与表示:JJF 1059.1—2012[S].北京:中国质检出版社,2012:1-25.General Administration of Quality Supervision.Evaluation and expression of uncertainty in measurement:JJF 1059.1—2012[S].Beijing:China Quality Inspection press,2012:1-25.
[14] 李慎安,王玉莲,范巧成.化学实验室测量不确定度[M].北京:化学工业出版社,2008:213-227.LI S A,WANG Y L,FAN Q C.Measurement uncertainty in chemical laboratory[M].Beijing:Chemical Industry Press,2008:213-227.
[15] 许禄,邵学广.化学计量学方法[M].北京:科学出版社,2006:163-176.XU L,SHAO X G.Chemometrics method[M].Beijing:Science Press,2006:163-176.
[16] 国家质量监督检验检疫总局.常用玻璃量器检定规程:JJG 196—2006[S].北京:中国质检出版社,2006:1-28.General Administration of Quality Supervision.Verification regulation of working glass container:JJG 196—2006[S].Beijing:China Quality Inspection press,2006:1-28.

Included in

Food Science Commons



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.