Simultaneous determination of 6 flavonoids in health care products by high performance liquid chromatography

Authors

WU Yi-qi, Guangzhou Inspection and Testing Certification Group Company Limited, Guangzhou,Guangdong 511447 , China ；National Quality Inspection and Testing Center for ProcessedFood 〔Guangdong〕, Guangzhou, Guangdong 511447 , China
LIANG Zhi-sen, Guangzhou Inspection and Testing Certification Group Company Limited, Guangzhou,Guangdong 511447 , China ；National Quality Inspection and Testing Center for ProcessedFood 〔Guangdong〕, Guangzhou, Guangdong 511447 , China
CHEN Yu-zhen, Guangzhou Inspection and Testing Certification Group Company Limited, Guangzhou,Guangdong 511447 , China ；National Quality Inspection and Testing Center for ProcessedFood 〔Guangdong〕, Guangzhou, Guangdong 511447 , China
ZHOU Rui, Guangzhou Inspection and Testing Certification Group Company Limited, Guangzhou,Guangdong 511447 , China ；National Quality Inspection and Testing Center for ProcessedFood 〔Guangdong〕, Guangzhou, Guangdong 511447 , China
LIN Xiao-jun, Guangzhou Inspection and Testing Certification Group Company Limited, Guangzhou,Guangdong 511447 , China ；National Quality Inspection and Testing Center for ProcessedFood 〔Guangdong〕, Guangzhou, Guangdong 511447 , China

Abstract

Objective: To establish a method for simultaneous determination of wogonoside, 4'-hydroxywogonin, norwogonin, baicalein, wogonin and chrysin in health care products by high performance liquid chromatography (HPLC). Methods: The samples were extracted with acetone, C₁₈ solid phase extraction column was used for purification. The target compounds were determined by HPLC, quantified by matrix-matched standard curve method. Phenomenex Luna^®C₁₈(250 mm×4.6 mm, 5 μm)column was applied, mobile phase was acctonitrile-acetic acid, using gradient elution)at the flow rate of 1.0 mL/min. The temperature of column was 30 ℃ and the detection wavelength was set at 280 nm. Results: The results showed that these 6 target compounds had good separation and the corresponding concentration had a good linear relationship with the peak area. Correlation coefficients (R²) were all greater than 0.99, RSDs of precision and stability tests were within 3.08% and the average recoveries were 83%~100% (RSD＜4.63%). The limits of detection were 0.02~0.07 mg/kg. The limits of quantification were 0.08~0.25 mg/kg. Conclusion: This method is suitable for simultaneous determination of flavonoids in health care products with good reproducibility, and less consumption of organic solvents.

Publication Date

10-16-2022

First Page

70

Last Page

75

DOI

10.13652/j.spjx.1003.5788.2022.90171

Recommended Citation

Yi-qi, WU; Zhi-sen, LIANG; Yu-zhen, CHEN; Rui, ZHOU; and Xiao-jun, LIN (2022) "Simultaneous determination of 6 flavonoids in health care products by high performance liquid chromatography," Food and Machinery: Vol. 38: Iss. 8, Article 13.
DOI: 10.13652/j.spjx.1003.5788.2022.90171
Available at: https://www.ifoodmm.cn/journal/vol38/iss8/13

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