•  
  •  
 

Abstract

Objective:This study focused on the antioxidant andimmune enhancing effects of L.bar barumpu ree.Methods:Theantioxidant capacity of L.bar barum puree was measured byes-tab lishing an acute liver injury model.The immune activity of L.bar barum protoplasmwas evaluated by measuring the index ofimmune organs, the content of serum hemolysin and the degreeof plantar thickening.Results:Both low and medium doses of L.bar barum puree could significantly increase the content of glut a-thione peroxidase(GSH PX) in serum(P<0.05, P<0.01) andglutathione(GSH) in liver tissue(P<0.05) , and significantlydecrease the content ofprotein carbonyl in liver tissue ofmicewith acute alcoholic liver injury(P<0.05, P<0.01)andthecontent of malondialdehyde(MDA) (P<0.05, P<0.01) , andthis indicate L.bar barum protoplasm hadantioxid antact ivity.After continuous gavage of L.barbarum protoplasm for 45 days,the organ development of normalm icewasn otaffected.Compared to the untreated mice, the content of serum hemolysin(P<0.01)and the degree of foot thickening(P<0.05)andphagocytosis index(P<0.01) increased significantly, indicatingthat L.bar barum puree could enhance mice immunity.Conclusion:Eating a certain dose of L.bar barum puree can en-hance the antioxidant capacity and immune ability.

Publication Date

10-16-2022

First Page

18

Last Page

21,28

DOI

10.13652/j.spjx.1003.5788.2022.90163

References

[1] 国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药出版社,2020:260.State Pharmacopoeia Committee.Pharmacopoeia of the people's re-publicof China:1st[M].Bejing:China Press of Traditional Chinese Medical,2020:260.
[2] 陈浩,张皓洁,师亮,等.枸杞多糖对帕金森病小鼠的抗氧化作用和神经保护效应[J].中国神经精神疾病杂志,2018,44(10):613-618.CHEN H,ZHANG HJ,SH IL,etal.Antioxidant and neuro protec-tive effects of Lycium bar barum polysaccharides on Parkinson'sdisease mice[J].Chinese Journal of Nervous and Mental Diseases,2018,44(10):613-618
[3] 陈博,宋小娜,宋彦,等.枸杞多糖对癫痫大鼠学习记忆能力的影响及抗氧化应激作用[J].中国中医药科技,2020,27(2):204-207.CHEN B,SONG XN,SONGY,etal.Effects of Lycium bar barumpolysaccharide on learning and memory ability and antioxidantstress in epileptic rats[J].Chinese Journal of Traditional MedicalScience and Technology,2020,27(2):204-207
[4] 马微.枸杞原浆制作方法研究[J].中国食品,2021,44(9):71-73.MAW.Study on preparation method of Lycium bar barum puree山.China Food,2021,44(9):71-73.
[5] 马晓娟,谢有发,余银芳,等.发酵枸杞原浆中多糖、活菌数的变化规律.食品安全导刊,2019,13(28):62-65.MAX J,XIE YF,YU YF,etal.Changes of polysaccharides andviable bacteria in fermented Lycium bar barum puree[J].China FoodSafety Magazine,2019,13(28):62-65.
[6] 沈垚垚.维生素C及柠檬酸对于鲜枸杞原浆的抗氧化研究[J]食品安全导刊,2020,14(36):132,134SHE NYY.Antioxidant effects of vitamin C and citric acid on freshLycium bar barum pulp[].China Food Safety Magazine,2020,14(36):132,134
[7] DEFREITAS RC,RAMOS BJ,VAL AND ROSM,etal.Gojiberry(Lycium barbar umL.)juice reduces lifespan and premature aging of Caenorhabditis elegans:Isitsafetoconsumeit?[J].FoodResIn t,2021,144:110297.
[8] LIU H,ZHOU X,HUANG S,etal.Lyciumbarbarumpolysaccha-rides and wolfberry juice prevent DEHP-induced hepatotoxicity viaPXR-regulated detoxification pathway[J].Molecules,2021,26(4):1-14
[9] WENG XQ,LIN BX,WANGCG,etal.The regulation of 100%Lycium Bar barum juice supplement in HPA axis and serum glucosebalance during increasing resistance training[J].Chin J Physiol,2021,37(5):482-485.
[10] 魏好程,邵杰,何传波,等.鲍内脏多糖体内抗氧化及增强小鼠免疫活性[J].食品科学,2018,39(9):140-144.WE IHC,SHAO J,HECB,etal.In vivo antioxidant capacity andimmuno enhancing activity in mice of polysaccharides fromabalone viscera[J].FoodScience,2018,39(9):140-144
[11] 刘志东,郭本恒,王荫榆.抗氧化活性检测方法的研究进展[刀天然产物研究与开发,2008,20(3):563-567LIU ZD,GUO BH,WANG YY.Methods to determine anti oxid ative activity[J].Natural Product Research and Development,2008,20(3):563-567.
[12] 马丽艳,胡昂,刘志东,等.扇贝抗氧化肽对衰老小鼠体内抗氧化活性研究口.食品安全质量检测学报,2018,9(19):51595163.MALY,HUA,LIU ZD,etal.Antioxidant function of peptidesfrom scallop by-product in aged mice in vivo[J].Journal of FoodSafety&Quality,2018,9(19):5159-5163.
[13] 张静丽,王宏勋,张雯,等.灵芝、枸杞多糖复合抗氧化作用[J]食品与机械,2004,20(6):11-12,46.ZHANG JL,WANG HX,ZHANG W,etal.Compoundantioxidant effect of Ganoderma lucidum and Lycium bar barumpolysaccharides[J.Food&Machinery,2004,20(6):11-12,46.
[14] V VOL PIN,TARU GIP.Influence of chondroitin sulfate chargedensity,sulfate group position,and molecular masson Cu²+-me-diated oxidation of human low-density lipoproteins:Effectofnor-mal human plasma derived chondroitin sulfate[J].J.Biochem,1999,125(2):297-304.
[15] LIU JD,LIU WB,ZHANGDD,etal.Dietary reduced glut at hi-one supplementation can improve growth,antioxidant capacity,and immunity on Chinese mitten crab,Er ioc heir sinensis[J].Fishand Shellfish Immunol,2020,100:300-308
[16] 王川南,吴天祥,雷露.不同年份酱香型白酒对小鼠抗疲劳及肝损伤试验的研究[J]酿酒科技,2019,40(10):36-40.WANG CN,WU TX,LEIL,etal.Study on anti fatigue and liverinjury of Mao taiBa i jiu liquor in different years J].Liquor-MakingScience&Technology,2019,40(10):36-40.
[17] 王诗洋,王枭,徐祖清,等.苦杏仁苷通过抑制氧化应激及炎症反应减轻四氯化碳诱导的大鼠肝纤维化作用].现代免疫学,2020,40(6):471-475,481.WANG SY,WANG X,XU ZQ,etal.Amy gdal in attenuatescarbon tetrachloride induced liver fibrosis in rats by inhibiting ox-i dative stress and inflammatory response[].Current Immunology,2020,40(6):471-475,481.
[18] EL-NEW ARY SA,SHAFF IE NM,O MERE A.The protection ofThymus vulgaris leaves alcoholic extract against hepatotoxicity ofalcohol in rats[J].Asian Pacific J of Tropical Med,2017,10(4):341-351.
[19] ABE K,KIMURA H.The possible role of hydrogen sulfide as anendogenous neuro modulator[J].Neur,1996,16(3):1066-1071.
[20] SAITO K,JINDH,OGAWA T,etal.Antioxidative properties oftripeptide libraries prepared by the combinatorial chemistry[J].JAgricul and Food Chem,2003,51(12):3668-3674.
[21] DAVALOS A,MIGUEL M,BARTOLOME B,etal.Antioxidantactivity of peptides derived from egg white proteins by enzymatichydrolysis[J].Food Protection,2004,67(9):1939-1944
[22] 罗晓航.PEF结合酶法提取鲍鱼脏器粗多糖及其抗氧化活性研究[D].福州:福建农林大学,2012:60-61LUO XH.Extraction of crude polysaccharide from abalone viscera byPEF combined with enzyme and its antioxidant activity[D].Fuzhou:Fu-jian Agriculture and Forestry University,2012:60-61
[23] 李晓梅,鞠玉琳.中药“FH”对小鼠血清溶血素和淋巴细胞转化的影响{J}.延边大学农学学报,2005,27(3):197-200.LI XM,JU YL.Effects of traditional Chinese medicine“FH"onserum hemolysin and lymphocyte transformation in mice山.Agri-cultural Science Journal of Yan bian University,2005,27(3):197-200.
[24] 孔晨先,肖波,柳全文,等.山药多糖对动物机体免疫调节作用的Meta分析{J}.食品研究与开发,2021,42(19):206-213.KONG CX,XIAO B,LIU QW,etal.Meta analysis of immuneregulation of yam polysaccharide on animals[J].Food Researchand Development,2021,42(19):206-213.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.