Preparation and analytical application of molecularly imprinted polymer electrochemical sensor forganoderic acid A

Authors

HUANG Gui-zhen, Minxi Vocational & Technical College, Longyan, Fujian 364021, ChinaFollow
WANG Qing-xiang, College of Chemistry, Chemical Engineering and Environment, Minnan Normal University, Zhangzhou, Fujian 363000, China
CHEN Jin-mei, College of Chemistry, Chemical Engineering and Environment, Minnan Normal University, Zhangzhou, Fujian 363000, China
ZHAN Feng-ping, College of Chemistry, Chemical Engineering and Environment, Minnan Normal University, Zhangzhou, Fujian 363000, China
WEI Lan, Minxi Vocational & Technical College, Longyan, Fujian 364021, China
ZHENG Wan-rong, Minxi Vocational & Technical College, Longyan, Fujian 364021, China

Abstract

Objective: Construction of a simple and rapid method for the analysis of ganoderic acid A （GAA）. Methods: The molecular imprinted polymer （MIP） that can specifically recognize ganoderic acid A （GAA） were prepared by cyclic voltammetric deposition using GAA as template molecule and o-phenylenediamine as functional monomer. The structure, morphology and electrochemical behavior of MIP were characterized by infrared spectroscopy, scanning electron microscopy and electrochemical methods. Using [Fe（CN）₆]^3-/4- as the active indicator, the analytical performance of the sensor for GAA was investigated. Porous MIP for GAA can be prepared on the electrode surface by one-step electropolymerization. Results: The results of quantitative analysis showed that the electrochemical signal of [Fe（CN）₆]^3-/4- had a good linear relationship with the logarithm of GAA concentration in the range of 1.0 pmol/L～1.0 μmol/L, and the detection limit was 0.21 pmol/L. The measured values of GAA in ethanol and water-soluble solution of Ganoderma lucidum powder were 1.15 nmol/L and 3.00 pmol/L, respectively. Conclusion: The MIP-based GAA electrochemical sensor can be used for the rapid determination of GAA in extract solution of Ganoderma lucidum powder.

Publication Date

4-25-2023

First Page

24

Last Page

30

DOI

10.13652/j.spjx.1003.5788.2022.80552

Recommended Citation

Gui-zhen, HUANG; Qing-xiang, WANG; Jin-mei, CHEN; Feng-ping, ZHAN; Lan, WEI; and Wan-rong, ZHENG (2023) "Preparation and analytical application of molecularly imprinted polymer electrochemical sensor forganoderic acid A," Food and Machinery: Vol. 39: Iss. 1, Article 4.
DOI: 10.13652/j.spjx.1003.5788.2022.80552
Available at: https://www.ifoodmm.cn/journal/vol39/iss1/4

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