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Corresponding Author(s)

郑喜群(1963—),男,黑龙江八一农垦大学教授,博士。E-mail:zhengxiqun@126.com王俊彤(1987—),女,黑龙江八一农垦大学讲师,博士。E-mail:wangjuntong1248@163.com

Abstract

Objective: This study aimed to provide a new direction for the application of protease restriction modification technique in corn protein. Methods: Corn glutelin was extracted from corn gluten meal. The response surface method was used to optimize the hydrolysis conditions of corn glutelin hydrolyzed by alcalase and protamex. The effects of different hydrolysates on the foam properties of corn glutelin and the additional amount on the quality of chiffon cake were investigated. Results: The optimal hydrolysis conditions for the protease alcalase and protamex on corn gluten were: pH 8, temperature 60 ℃, enzyme dosage 3%, pH 7, temperature 50 ℃, and enzyme dosage 0.81%,respectively. The foaming property and foam stability of hydrolysates were increased. When the amount of alcalase hydrolysate and protamex hydrolysate were 10% and 30%, respectively. The texture characteristics of chiffon cake were the best. At this time, the expansion degree of chiffon cake reached the highest, the hardness and chewability reached the lowest, and the elasticity and recovery reached the maximum. In addition, in this addition amount, its chroma was the closest to that of the 0-addition group. Conclusion: Corn glutelin can be effectively hydrolyzed by alcalase and protamex to obtain glutelin hydrolysates with good foaming properties, which will be beneficial to expand its application in the food industry.

Publication Date

12-26-2023

First Page

197

Last Page

205,216

DOI

10.13652/j.spjx.1003.5788.2023.80141

References

[1] 高婷婷, 谭雪, 史海慧, 等. 试论玉米蛋白粉应用现状及质量控制[J]. 粮食问题研究, 2018(6): 29-33. GAO T T, TAN X, SHI H H, et al. Discussion on application status and quality control of corn protein powder[J]. Grain Issues Research, 2018(6): 29-33.
[2] 陈静, 陈志宏, 张汆, 等. 响应面法优化玉米蛋白粉酶解工艺及其动力学研究[J]. 武汉轻工大学学报, 2020, 39(5): 9-16. CHEN J, CHEN Z H, ZHANG C, et al. Study on enzymolysis process optimization of corn gluten meal by response surface methodology and its enzymatic hydrolysis kinetic[J]. Journal of Wuhan Polytechnic University, 2020, 39(5): 9-16.
[3] 刘金玲, 刘晓兰, 郑喜群. 转谷氨酰胺酶催化壳寡糖糖基化修饰玉米谷蛋白[J]. 食品科技, 2015(9): 7-11, 16. LIU J L, LIU X L, ZHENG X Q. TGase-catalyzed glycosylation of corn glutelin with chitosan oligochitosan[J]. Food Science and Technology, 2015(9): 7-11, 16.
[4] 韩永霞. 食物蛋白质与人体免疫力[J]. 食品安全导刊, 2020(36): 45, 47. HAN Y X. Food protein and human immunity[J]. China Food Safety Magazine, 2020(36): 45, 47.
[5] 何娇娇, 王萍, 冯建, 等. 玉米蛋白粉对大黄鱼生长、肠道组织结构及肠道菌群的影响[J]. 中国水产科学, 2018, 25(2): 361-372. HE J J, WANG P, FENG J, et al. Effects of replacing fish meal with corn gluten meal on growth, intestinal histology, and intestina microbiota of large yellow croaker, Larimichthys crocea[J]. Journal of Fishery Sciences of China, 2018, 25(2): 361-372.
[6] 景言, 刘晓兰, 王今雨, 等. 玉米蛋白水解物的酶法制备及其对结肠炎的缓解作用[J]. 食品工业科技, 2023, 44(8): 270-278. JING Y, LIU X L, WANG J Y, et al. Enzymatic preparation of corn protein hydrolysate and its alleviating effect on colitis[J]. Science and Technology of Food Industry, 2023, 44(8): 270-278.
[7] 王欢, 迟玉杰, 王晓莹, 等. 蛋清粉起泡性对戚风蛋糕品质的影响[J]. 中国家禽, 2014, 36(20): 34-38. WANG H, CHI Y J, WANG X Y, et al. Effect of foaming properties of egg white powder on the quality of chiffon cake[J]. China Poultry, 2014, 36(20): 34-38.
[8] 张敏华, 郑宗平, 陈洪彬, 等. 淮山粉对木糖醇戚风蛋糕品质的影响[J]. 食品与机械, 2022, 38(8): 182-187. ZHANG M H, ZHENG Z P, CHEN H B, et al. Effects of yam powder on the quality of xylitol chiffon cake[J]. Food & Machinery, 2022, 38(8): 182-187.
[9] TINCHAN P, SIRIJSRIYSWAT A, PROMMAKOOL A, et al. Antidesma thwaitesianum Müll. Arg. fruit juice, its phytochemical contents, antimicrobial activity, and application in chiffon cake[J]. International Journal of Food Science, 2022, 85(3): 5183562.
[10] 任传顺, 郑洋洋, 崔玲玲, 等. 鸡蛋的储存条件对大米戚风蛋糕品质特性的影响[J]. 河南工业大学学报(自然科学版), 2021, 42(2): 43-49. REN C S, ZHENG Y Y, CUI L L, et al. Effects of egg storage conditions on the qualities of rice chiffon cake[J]. Journal of Henan University of Technology (Natural Science Edition), 2021, 42(2): 43-49.
[11] 李鑫. 基于界面特性解析蛋清蛋白体系泡沫性质及其调控机理研究[D]. 无锡: 江南大学, 2020: 1-11. LI X. Engineered interface for explaining foaming characteristics of egg white protein systems and regulation mechanisms[D]. Wuxi: Jiangnan University, 2020: 1-11.
[12] WANG Y, ZHANG Z, HE R H, et al. Proteolysis efficiency and structural traits of corn gluten meal: Impact of different frequency modes of a low-power density ultrasound[J]. Food Chemistry, 2021, 344: 128609.
[13] WANG Y H, LIN Y, YANG X Q. Foaming properties and air-water interfacial behavior of corn protein hydrolysate-tannic acid complexes[J]. Journal of Food Science and Technology, 2019, 56(2): 905-913.
[14] ZHAO M, XIONG W, CHEN B, et al. Enhancing the solubility and foam ability of rice glutelin by heat treatment at pH 12: Insight into protein structure[J]. Food Hydrocolloids, 2019, 103: 105626.
[15] 许瑞雪, 刘晓兰, 王俊彤, 等. 体外消化对玉米谷蛋白酶解物抗氧化活性的影响[J]. 食品研究与开发, 2016, 37(16): 1-5. XU R X, LIU X L, WANG J T, et al. Effect of in vitro digestion on the antioxidant activities of hydrolysate from corn glutelin[J]. Food Research and Development, 2016, 37(16): 1-5.
[16] YIN S W, TANG C H. Effects of limited enzymatic hydrolysis with trypsin on the functional properties of hemp (Cannabis sativa L.) protein isolate[J]. Food Chemistry, 2008, 106(3): 1 004-1 013.
[17] 李天娇, 徐响, 孙丽萍, 等. 油菜蜂花粉蛋白水解度测定方法比较[J]. 食品科学, 2010, 31(16): 184-187. LI T J, XU X, SUN L P, et al. Comparison of methods for determining the hydrolysis degree of rape bee pollen protein[J]. Food Science, 2010, 31(16): 184-187.
[18] 李波轮, 仝召莉, 王新宇, 等. 糊粉层添加量对土司面包品质特性的影响[J]. 粮食加工, 2022, 47(4): 12-17. LI B L, TONG Z L, WANG X Y, et al. Effects of addition of aleurone layer on the quality characteristics of toast bread[J]. Grain Processing, 2022, 47(4): 12-17.
[19] 刘浩男. 高功率脉冲微波对大豆分离蛋白和蓝莓花色苷互作机理及其复合物特性的影响研究[D]. 镇江: 江苏大学, 2022: 43. LIU H N. Effects of high power pulsed microwave on the interaction mechanism between soy protein isolate and biuberry anthocyanin and properties of complex[D]. Zhenjiang: Jiangsu University, 2022: 43.
[20] LIANG Q F, REN X F, CHALAMAIAH M, et al. Simulated gastrointestinal digests of corn protein hydrolysate alleviate inflammation in caco-2 cells and a mouse model of colitis[J]. Journal of Food Science and Technology, 2020, 57(6): 2 079-2 088.
[21] 赵婉宏, 郑喜群, 刘晓兰, 等. 双酶复合两步水解法制备玉米降血糖活性肽及其生物活性[J]. 中国粮油学报, 2022, 37(10): 221-229. ZHAO W H, ZHENG X Q, LIU X L, et al. Preparation of corn hypoglycemic active peptide by double enzyme compound two-step hydrolysis and its biological activity[J]. Journal of the Chinese Cereals and Oils Association, 2022, 37(10): 221-229.
[22] 郑志强, 郝利民, 刘晋, 等. 小麦蛋白双酶酶解制备高抗氧化性小麦肽研究[J]. 中国食品学报, 2019, 19(8): 78-88. ZHENG Z Q, HAO L M, LIU J, et al. Preparation of wheat peptide with high antioxidant activity by double enzymes hydrolysis of wheat gluten[J]. Journal of Chinese Institute of Food Science and Technology, 2019, 19(8): 78-88.
[23] KLOST M, DRUSCH S. Functionalisation of pea protein by tryptic hydrolysis-characterisation of interfacial and functional properties[J]. Food Hydrocolloids, 2018, 86: 134-140.
[24] WOUTERS A G B, ROMBOUTS I, FIERENS E, et al. Foaming and air-water interfacial characteristics of solutions containing both gluten hydrolysate and egg white protein[J]. Food Hydrocolloids, 2018, 77: 176-186.
[25] JUAN M, RODRIGUEZ P, JOS M C, et al. Interfacial and foaming properties of enzyme-induced hydrolysis of sunflower protein isolate[J]. Food Hydrocolloids, 2006, 21(5/6): 782-793.
[26] REN J, SONG C L, ZHANG H Y, et al. Effect of hydrolysis degree on structural and interfacial properties of sunflower protein isolates[J]. Journal of Food Processing and Preservation, 2016, 41(1): e13092.
[27] 罗其琪, 顾丰颖, 曹晶晶, 等. 鼠李糖乳杆菌发酵对玉米粉、玉米面团理化特性及发糕品质的影响[J]. 食品科学, 2018, 39(18): 1-7. LUO Q Q, GU F Y, CAO J J, et al. Effects of lactobacillus rhamnosus fermentation on the physicochemical properties of corn flour and corn dough and the quality of steamed sponge cake[J]. Food Science, 2018, 39(18): 1-7.
[28] 张佳佳, 王昱丹, 罗慧, 等. 蒲公英戚风蛋糕的烘焙品质及其酚类物质抗氧化活性[J]. 食品与发酵工业, 2019, 45(4): 142-146. ZHANG J J, WANG Y D, LUO H, et al. Effect of dandelion flour on baking qualities and polyphenols antioxidant activities of chiffon cake[J]. Food and Fermentation Industries, 2019, 45(4): 142-146.
[29] NING J, HOU G G, SUN J, et al. Effect of green tea powder on the quality attributes and antioxidant activity of whole-wheat flour pan bread[J]. LWT-Food Science and Technology, 2017, 79: 342-348.
[30] 朱琳, 陈丽丽, 袁美兰, 等. 草鱼多肽的营养价值及添加鱼蛋白面包的研制[J]. 食品工业, 2017, 38(8): 125-129. ZHU L, CHEN L L, YUAN M L, et al. Nutritional value estimate of grass carp peptides and process of added fish protein into bread[J]. The Food Industry, 2017, 38(8): 125-129.
[31] 李雪颖, 周鸿燕, 武媛媛. 大豆蛋白水解物对面包体外抗氧化及品质特性的影响[J]. 粮食与油脂, 2021, 34(8): 21-23, 33. LI X Y, ZHOU H Y, WU Y Y. Effect of soybean protein hydrolysate on antioxidant in vitro and quality characteristics of bread[J]. Cereals & Oils, 2021, 34(8): 21-23, 33.
[32] 杨晓钒, 王炜清, 李秀婷, 等. 酶解处理对扁桃仁蛋白质起泡特性影响及其应用研究[J]. 食品科学技术学报, 2021, 39(5): 82-92. YANG X F, WANG W Q, LI X T, et al. Study on effect of enzymatic hydrolysis on foaming properties of almond protein isolate and its application[J]. Journal of Food Science and Technology, 2021, 39(5): 82-92.

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