Abstract
Objective: An ultra-high performance liquid chromatography-tandem mass spectrometry method was established for the simultaneous determination of 27 antiparasitic drug residues in Litopenaeus vannamei. Methods: The solution was used to extract antiparasitic drugs in the samples of L. vannamei. The supernatant after centrifugation was purified by C18 and neutral alumina powder. The sample solution was analyzed by ultra-high performance liquid chromatography-tandem mass spectrometry and applied to the instrument workstation to perform data analysis. A Thermo Hypersil Gold aQ column was used as the separation column, and 0.1% formic acid acetonitrile and 10 mmol/L ammonium formate solution （containing 0.1% formic acid） were invoked as the organic phase and the aqueous phase for gradient elution, respectively. The electrospray ion source was used for positive and negative ion synchronous scanning and multiple reaction monitoring modes. The ion abundance ratio was qualitative, and the matrix-matched reference solution external standard method was employed for quantitative analysis. The results showed that the Pearson correlation coefficient （r） of 27 antiparasitic drugs was greater than 0.974 1 in a certain concentration range, and the linear relationship was good. Results: The detection limit was 0.5～2.0 μg/kg, and the quantification limit was 2～5 μg/kg; The recoveries （n=6） of the method spiked at the low, medium and high concentration levels of the samples were 61.90%～118.54%, the intra-assay coefficient of variation （n=6） was 0.57%～12.56%, and the inter-assay coefficient of variation （n=3） was 1.29%～16.47%. Conclusion: The method has good selectivity, high method recovery rate, high precision, and short measurement period, provides a robust and efficient method for the determination of antiparasitic drugs in the laboratory, and meets the testing needs of the laboratory.
Publication Date
12-26-2023
First Page
64
Last Page
71
DOI
10.13652/j.spjx.1003.5788.2023.80077
Recommended Citation
Lifang, ZHANG; Ruixin, MA; Baiqin, ZHENG; Wang, SONG; and Xin, ZHOU
(2023)
"Determination of 27 antiparasitic drug residues in Penaeus vannamei by liquid chromatography-tandem mass spectrometry simultaneously,"
Food and Machinery: Vol. 39:
Iss.
11, Article 11.
DOI: 10.13652/j.spjx.1003.5788.2023.80077
Available at:
https://www.ifoodmm.cn/journal/vol39/iss11/11
References
[1] 刘永涛, 余琳雪, 王桢月, 等. 改良的QuEChERS结合高效液相色谱—串联质谱同时测定水产品中7种阿维菌素类药物残留[J]. 色谱, 2017, 35（12）: 1 276-1 285.
LIU Y T, YU L X, WANG Z Y, et al. Simultanenous determination of sever avermectin residues in aquatic products by modified QuEChERS combined with high-performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2017, 35（12）: 1 276-1 285.
[2] 邢丽红, 孙伟红, 孙晓杰, 等. 分散固相萃取净化—高效液相色谱—串联质谱法测定鱼体中地克珠利残留量[J]. 食品科学, 2016, 37（14）: 174-179.
XING L H, SUN W H, SUN X J, et al. Determination of diclazuril in fish by high performance liquid chromatography-tandem mass spectrometry with dispersive solid phase extraction[J]. Food Science, 2016, 37（14）: 174-179.
[3] 林海丹, 林峰, 张美金, 等. 高效液相色谱法同时测定动物组织中16种苯并咪唑类药物残留[J]. 食品科学, 2011, 32（2）: 231-236.
LIN H D, LIN F, ZHANG M J, et al. Simultaneous HPLC determination of 16 benzimidazoles residuesin animal tissue[J]. Food Science, 2011, 32（2）: 231-236.
[4] GAN J, LIU H, CHEN Y, et al. One step extraction followed by HPLC-ESI-MS/MS for multi-residue analysis of diacylhydrazine insecticides in water, sediment, and aquatic products[J]. Ecotoxicology and Environmental Safety, 2021, 210: 111853.
[5] KONG C, WANG Y, HUANG Y, et al. Multiclass screening of >200 pharmaceutical and other residues in aquatic foods by ultrahigh-performance liquid chromatography-quadrupole-orbitrap mass spectrometry[J]. Analytical and Bioanalytical Chemistry, 2018, 410（22）: 5 545-5 553.
[6] CARABAJAL M, TEGLIA C M, MAINE M A, et al. Multivariate optimization of a dispersive liquid-liquid microextraction method for the determination of six antiparasite drugs in kennel effluent waters by using second-order chromatographic data[J]. Talanta, 2021, 224: 121929.
[7] UCHIDA K, KONISHI Y, HARADA K, et al. Monitoring of antibiotic residues in aquatic products in urban and rural areas of vietnam[J]. Journal of Agricultural and Food Chemistry, 2016, 64（31）: 6 133-6 138.
[8] OKOCHA R C, OLATOYE I O, ADEDEJI O B. Food safety impacts of antimicrobial use and their residues in aquaculture[J]. Public Health Reviews, 2018, 39: 21.
[9] HANNISDAL R, NOSTBAKKEN O J, HOVE H, et al. Anti-sea lice agents in Norwegian aquaculture; surveillance, treatment trends and possible implications for food safety[J]. Aquaculture, 2020, 521: 735044.
[10] DA L N, HOANG A Q, HOANG T T H, et al. Antibiotic and antiparasitic residues in surface water of urban rivers in the red river delta （Hanoi, Vietnam）: Concentrations, profiles, source estimation, and risk assessment[J]. Environmental Science and Pollution Research, 2021, 28（9）: 10 622-10 632.
[11] KANG H, LEE S, SHIN D, et al. Occurrence of veterinary drug residues in farmed fishery products in South Korea[J]. Food Control, 2018, 85: 57-65.
[12] TURNIPSEED S B, STOREY J M, WU I L, et al. Extended liquid chromatography high resolution mass spectrometry screening method for veterinary drug, pesticide and human pharmaceutical residues in aquaculture fish[J]. Food Addit Contam Part A Chem Anal Control Expo Risk Assess, 2019, 36（10）: 1 501-1 514.
[13] 吴增茹, 朱丽荔, 骆宏鹏, 等. 伊维菌素在电喷雾质谱中的行为及其机理的研究[J]. 质谱学报, 2001（2）: 1-9.
WU Z R, ZHU L L, LUO H P, et al. Studies on the behavior and mechanisms of ivermectin in electrospray ionization mass spectrometry[J]. Journal of Chinese Mass Spectrometry Society, 2001（2）: 1-9.
[14] 岳振峰, 康海宁, 陈小霞, 等. 液相色谱串联质谱法测定鸡肉中20种抗球虫药物多残留[J]. 分析化学, 2012, 40（8）: 1 262-1 266.
YUE Z F, KANG H N, CHEN X X, et al. Simultaneous determination of 20 coccidiostat residues in chicken by liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Analytical Chemistry, 2012, 40（8）: 1 262-1 266.
[15] 刘开, 郭建博, 薛晓文, 等. 超高效液相色谱—串联质谱法测定动物源性食品中硝基咪唑类药物及其代谢物的残留量的研究[J]. 食品安全质量检测学报, 2020, 11（19）: 6 751-6 758.
LIU K, GUO J B, XUE X W, et al. Determination of nitroimidazoles and their metabolites residues in foodstuffs of animal origin by ultra performance liquid chromatography-tandem mass spectrometry[J]. Journal of Food Safety and Quality, 2020, 11（19）: 6 751-6 758.
[16] 陈思敏, 吴映璇, 蓝草, 等. 超高效液相色谱—串联质谱法测定乳制品中苯并咪唑类药物及其代谢物的残留量[J]. 理化检验: 化学分册, 2020, 56（5）: 553-564.
CHEN S M, WU Y X, LAN C, et al. UHPLC-MS/MS determination of residual amounts of benzimidazoles and its metabolites in dairy product[J]. Physical Testing and Chemical Analysis Part B: Chemical Analysis, 2020, 56（5）: 553-564.
[17] 刘晓云, 陈笑艳, 钟大放. 液相色谱—串联质谱生物分析方法的基质效应和对策[J]. 质谱学报, 2017, 38（4）: 388-399.
LIU X Y, CHEN X Y, ZHONG D F. Matrix effects and countermeaasure of liquid chromatography-tandem mass spectrometry in bioanalys[J]. Journal of Chinese Mass Spectrometry Society, 2017, 38（4）: 388-399.
[18] BERNARDES A N, GODOI L A G, CAMILOTI P R, et al. Organic acids and alcohols quantification by HPLC/RID in sugarcane vinasse: Analytical method validation and matrix effect assessment[J]. International Journal of Environmental Analytical Chemistry, 2021, 101（3）: 325-336.
[19] 张颖. QuEChERS结合UPLC-MS/MS测定水产品中兽药残留的研究[D]. 舟山: 浙江海洋大学, 2021: 21-22.
ZHANG Y. Research and application fo QuEChERS on veterinary drug residue in animal-derived food[D]. Zhoushan: Zhejiang Ocean University, 2021: 21-22.
[20] 黄胜广, 王玉方, 赵卉, 等. QuEChERS/UPLC-MS/MS法同时测定梅花鹿鹿茸中36种兽药残留[J]. 质谱学报, 2020, 41（6）: 648-658.
HUANG S G, WANG Y F, ZHAO H, et al. Determination of 36 veterinary drug residues in antler velvet of Cervus nippon Temminck by QuEChERS/UPLC-MS/MS[J]. Journal of Chinese Mass Spectrometry Society, 2020, 41（6）: 648-658.