Abstract
Objective: This study aimed to establish a method by using QuEChERS-gas chromatography tandem mass spectrometry (GC-MS/MS), to determine six pesticide residues in tea, including: hydroprene, kinoprene, genite, fluronitrofen, cycloprate, and chlornitrofen. Methods: The samples were pretreated by QuEChERS method. They were separated by gas chromatographic, detected by multiple reaction monitoring (MRM) mode and quantified by the external method. Results: All the pesticide residues showed good linearity with correlation coefficients above 0.999 when the concentration falls in the range 0.025~2.000 mg/kg, and the limits of quantitation were in the range of 0.000 6 ~0.007 2 mg/kg. In the recovery experiment of 0.002 5 mg/kg, 0.05 mg/kg, and 0.5 mg/kg at three concentrations, the average recoveries were 84.3%5~116.3%, and the relative standard deviations (RSDs) were 1.07%~6.05%. Conclusion: The method is rapid, simple, and accurate, which is suitable for the detection of these six pesticide residues in tea.
Publication Date
10-20-2023
First Page
55
Last Page
58,111
DOI
10.13652/j.spjx.1003.5788.2023.80157
Recommended Citation
Han-bin, WU
(2023)
"Simultaneous determination of 6 pesticide residues in tea by QuEChERS-GS-MS/MS,"
Food and Machinery: Vol. 39:
Iss.
6, Article 9.
DOI: 10.13652/j.spjx.1003.5788.2023.80157
Available at:
https://www.ifoodmm.cn/journal/vol39/iss6/9
References
[1] 韦友欢, 黄秋婵, 陆维坤. 解读茶叶与人体健康[J]. 广东茶业, 2008（1）: 24-27.
WEI Y H, HUANG Q C, LU W K. Tea and Human health[J]. Guangdong Tea, 2008（1）: 24-27.
[2] 张翠华, 张亚楠. 固相微萃取在农药残留检测中的应用研究[J]. 沧州师范学院学报, 2017, 33（2）: 29-34.
ZHANG C H, ZHANG Y N. On the application of solid-phase microextraction in detecting pesticide residue[J]. Journal of Cangzhou Normal University, 2017, 33（2）: 29-34.
[3] 胡贝贞, 宋伟华, 谢丽萍, 等. 加速溶剂萃取/凝胶渗透色谱—固相萃取净化/气相色谱—质谱法测定茶叶中残留的33种农药[J]. 色谱, 2008（1）: 22-28.
HU B Z, SONG W H, XIE L P, et al. Determination of 33 pesticides in tea by accelerated solvent extraction-gelperm eation and solid-phase extraction purification-gas chromatography-mass spectrometry[J]. Chinese Journal of Chromatograph, 2008（1）: 22-28.
[4] 段兵, 何太喜, 范媛媛, 等. 凝胶渗透色谱—超高效液相色谱—串联质谱测定茶叶中吡虫啉和啶虫脒[J]. 食品科学, 2016, 37（4）: 238-241.
DUAN B, HE T X, FAN Y Y, et al. Detemination of imidacloprid and acetamiprid in tea by gel permeation chromatography-ultra performance liquid chmmatography-tandem mass spectrometry[J]. Food Scienoe, 2016, 37（4）: 238-241.
[5] 曹春霞, 黄大野, 吴琼, 等. 茶叶农药残留检测分析方法研究进展[J]. 湖北农业科学, 2019, 58（17）: 5-8, 12.
CAO C X, HUANG D Y, WU Q, et al. Research progress of determination methods of pesticide residues in tea[J]. Hubei Agricultural Sciences, 2019, 58（17）: 5-8, 12.
[6] 刘妍慧, 于常红, 刘岩, 等. 基质固相分散萃取—高效液相色谱串联质谱法（HPLC-MS-MS）同时测定茶叶中11种农药的残留量[J]. 茶叶科学, 2014, 34（3）: 271-278.
LIUY H, YU C H, LIU Y et al. Simultaneous determination of 11 kinds of pesticide residues in tea by HPLC-MS-MS[J]. Journal of Tea Science, 2014, 34（3）: 271-278.
[7] 官金艳, 董军, 韩艳云. 茶叶中农药多残留测定的样品前处理方法综述[J]. 湖北农业科学, 2020, 59（S1）: 20-27.
GUAN J Y, DONG J, HAN Y Y, et al. Summary of sample pretreatment for determination of pesticide residues in tea[J]. Hubei Agricultural Sciences, 2020, 59（S1）: 20-27.
[8] 刘满满, 康澍, 姚成. QuEChERS方法在农药多残留检测中的应用研究进展[J]. 农药学学报, 2013, 15（1）: 8-22.
LIU M M, KANG S, YAO C. Research progress of QuEChERS method in pesticide mult-residue analysis[J]. Chinese Journal of Pesticide Science, 2013, 15（1）: 8-22.
[9] 张媛媛, 张卓, 陈忠正, 等. QuEChERS方法在茶叶农药残留检测中的应用研究进展[J]. 食品安全质量检测学报, 2014, 5（9）: 2 711-2 716.
ZHANG Y Y, ZHANG Z, CHEN Z Z, et al. QuEChERS methodology and its application in pesticide residues determination in tea[J]. Journal of Food safety and Quality, 2014, 5（9）: 2 711-2 716.
[10] 高阳, 徐应明, 孙扬, 等. QuEChERS 提取法在农产品农药残留检测中的应用进展[J]. 农业资源与环境学报, 2014, 31（2）: 110-117.
GAO Y, XU Y M, SUN Y, et al. Research progress of QuEChERS extraction method in pesticide residues determination in agricultural products[J]. Journal of Agricultural Resources and Environment, 2014, 31（2）: 110-117.
[11] 刘华文, 苏海雁, 陆小康, 等. QuEChERS/超高效液相色谱—串联质谱法测定茶叶中28种农药残留[J]. 食品工业科技, 2021, 42（2）: 223-236.
LIU H W, SU H Y, LU X K, et al. Determination of 28 kinds of pesticide pesidues in tea by QuEChERS /Ultra performance liquid chromatography-tandem mass spectrometry[J]. Science and Technology of Food Industy, 2021, 42（2）: 223-236.
[12] 贺利民, 刘祥国, 曾振灵. 气相色谱分析农药残留的基质效应及其解决方法[J]. 色谱, 2008（1）: 98-104.
HE L M, LIU X G, ZENG Z L. Solutions to matrix-induced response enhancement in pesticide residue analysis by gas chromatography[J]. Chinese Journal of Chromatograph, 2008（1）: 98-104.
[13] 满红平, 吕生, 毛静春. 气相色谱—串联质谱法测定普洱晒青毛茶中192种农药残留量[J]. 食品安全质量检测学报, 2019, 10（9）: 2 702-2 722.
MAN H P, LU S, MAO J C, et al. Determination of 192 kinds of pesticide residues in Pu'er tea by gas chromatography tandem mass spectrometry[J]. Journal of Food safety and Quality, 2019, 10（9）: 2 702-2 722.