Simultaneous determination of 21 perfluorinated compounds in animal-derived food by UPLC-MS/MS

Authors

TANG Yun-xi, Chongqing Institute for Food and Drug Control, Chongqing 401120, China;State Key Laboratory of Market Regulation 〔Condiment Supervision Technology〕, Chongqing 401120, China
BAI Ya-min, Chongqing Institute for Food and Drug Control, Chongqing 401120, China;State Key Laboratory of Market Regulation 〔Condiment Supervision Technology〕, Chongqing 401120, ChinaFollow
LIU Lu-lu, Chongqing Institute for Food and Drug Control, Chongqing 401120, China;State Key Laboratory of Market Regulation 〔Condiment Supervision Technology〕, Chongqing 401120, China
YU Jie, Chongqing Institute for Food and Drug Control, Chongqing 401120, China;State Key Laboratory of Market Regulation 〔Condiment Supervision Technology〕, Chongqing 401120, China

Corresponding Author(s)

白亚敏（1977—），女，重庆市食品药品检验检测研究院高级工程师，硕士。E-mail：965241582@qq.com

Abstract

Objective: To established a method for determination of 21 perfluorinated compounds in animal-derived food by ultra-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）. Methods: The sample was extracted with 1% formic acid-acetonitrile, cleaned up with a sorbent mixture of N-propylethylenediamine （PSA）, octadecyl bonded silica gel （C₁₈） and graphitized carbon black （GCB）, then separated by acetonitrile-5mmol/L ammonium acetate aqueous solution and Acquity UPLC^ BEH C₁₈, detected by liquid chromatography tandem mass spectrometry in negative mode using the multiple reaction monitoring （MRM） mode. Results: The calibration curves were linear in the range of 0.2~10 ng/mL for the 21 PFAS. The limits of quantification were 0.25 μg/kg. Average recoveries were in the range of 71%~118% with three spiked levels, namely 0.25, 0.50, 1.25 μg/kg. The relative standard deviations （RSDs%） of inter-batch and intra-batch were 3.5%~18.3% （n=6） and 2.2%~13.8% （n=3）, respectively. Conclusion: The simple pretreatment and high sensitivity satisfied the fast and accurate detection requirement, meanwhile it is suitable for qualitative and quantitative analysis of perfluorinated compounds in animal-derived food.

Publication Date

10-20-2023

First Page

29

Last Page

39

DOI

10.13652/j.spjx.1003.5788.2022.81188

Recommended Citation

Yun-xi, TANG; Ya-min, BAI; Lu-lu, LIU; and Jie, YU (2023) "Simultaneous determination of 21 perfluorinated compounds in animal-derived food by UPLC-MS/MS," Food and Machinery: Vol. 39: Iss. 7, Article 5.
DOI: 10.13652/j.spjx.1003.5788.2022.81188
Available at: https://www.ifoodmm.cn/journal/vol39/iss7/5

References

[1] HUERTA B, MCHUGH B, REGAN F. Development and application of an LC-MS method to the determination of poly- and perfluoroalkyl substances （PFASs） in drinking, sea and surface water samples[J]. Anal Methods, 2022, 14（21）: 2 090-2 099.
[2] 宋彦敏, 周连宁, 郝文龙, 等. 全氟化合物的污染现状及国内外研究进展[J]. 环境工程, 2017, 35（10）: 82-86. SONG Y M, ZHOU L N, HAO W L, et al. Pollution status and related research progress of perfluorinated compounds[J]. Environmental Engineering, 2017, 35（10）: 82-86.
[3] 陈雷, 戴玙芽, 陈晓婷, 等. 全氟及多氟化合物在土壤中的污染现状及环境行为研究进展[J]. 农业环境科学学报, 2021, 40（8）: 1 611-1 622. CHEN L, DAI Y Y, CHEN X T, et al. Research progress on the pollution status and environmental behavior of per-and polyfluoroalkyl substances in soil[J]. Journal of Agro-Environment Science, 2021, 40（8）: 1 611-1 622.
[4] 郑宇. 典型城市大气中全氟化合物的污染特征[D]. 青岛: 青岛大学, 2020: 2-3. ZHENG Y. Contaminant characteristics of perfluorinated compounds in atmospheric of typical cities[D]. Qingdao: Qingdao University, 2020: 2-3.
[5] 孙玉珠, 李建宇, 武梦茹, 等. 动物性食品中全氟烷基物质的残留现状[J]. 中兽医医药杂志, 2019, 38（5）: 29-33. SUN Y Z, LI J Y, WU M R, et al. Residue status of perfluoroalkyl substances in animal foods[J]. Journal of Traditional Chinese Veterinary Medicine, 2019, 38（5）: 29-33.
[6] 高灵飞, 王惟儒, 刘文欣, 等. 全氟/多氟烷基化合物在水环境中污染现状[J]. 环境科学与技术, 2020, 43（7）: 160-168. GAO L F, WANG W R, LIU W X, et al. Pollution status of per-and polyfluoroalkyl substances in water environment[J]. Environmental Science & Technology, 2020, 43（7）: 160-168.
[7] 杜国勇, 蒋小萍, 卓丽, 等. 长江流域重庆段水体中全氟化合物的污染特征及风险评价[J]. 生态环境学报, 2019, 28（11）: 2 266-2 272. DU G Y, JIANG X P, ZHUO L, et al. Distribution characteristics and risk assessment of perfluorinated compounds in surface water from Chongqing Section of the yangtze river[J]. Ecology and Environmental Sciences, 2019, 28（11）: 2 266-2 272.
[8] 刘嘉烈, 石运刚, 唐娜, 等. 重庆长江流域鲫鱼和沉积物中17种全氟化合物污染特征[J]. 环境化学, 2020, 39（12）: 3 450-3 461. LIU J L, SHI Y G, TANG N, et al. Pollution characteristics of seventeen per- and polyfluoroalkyl substances in fish and sediments of yangtze river basin in Chongqing city[J]. Environmental Chemistry, 2020, 39（12）: 3 450-3 461.
[9] WANG X L, ZHANG Q C, ZHAO Z Y, et al. A multi-plug filtration （m-PFC） cleanup method based on carboxylic multi-walled carbon nanotubes for the detection of 14 perfluorinated compounds and dietary risk assessment of chicken, beef, and mutton collected from Shanghai markets[J]. Food Control, 2021, 130: 1-10.
[10] QI X, ZHOU J, WANG M, et al. Perfluorinated compounds in poultry products from the yangtze river delta and pearl river delta regions in China[J]. Science of the Total Environment, 2019, 689: 1 079-1 086.
[11] LI P Y, OYANG X H, ZHAO Y L, et al. Occurrence of perfluorinated compounds in agricultural environment, vegetables, and fruits in regions influenced by a fluorine-chemical industrial park in China[J]. Chemosphere, 2019, 225: 659-667.
[12] CHUNG S M, HEO D G, KIM J H, et al. Perfluorinated compounds in adults and their association with fasting glucose and incident diabetes: A prospective cohort study[J]. Environmental Health, 2022, 21（1）: 101.
[13] 张露露, 刘婧, 贺颖倩, 等. 血清全氟化合物与代谢相关脂肪性肝病患病风险的关系[J]. 中国环境科学, 2023, 43（2）: 964-972. ZHANG L L, LIU Q, HE Y Q, et al. The relationship of serum perfluoroalkyl substances with the risk of metabolic associated fatty liver disease[J]. China Environmental Science, 2023, 43（2）: 964-972.
[14] 郭聪慧, 范玉珊, 乔延召, 等. 全氟类化合物对雌性哺乳动物的生殖毒性[J]. 动物医学进展, 2020, 41（12）: 120-123. GUO C H, FAN Y S, QIAO Y Z, et al. Reproductive toxicity of perfluorinated compounds in female mammals[J]. Progress in Veterinary Medicine, 2020, 41（12）: 120-123.
[15] KNUTSEN H K, ALEXANDER J, BARREGRD L, et al. Risk to human health related to the presence of perfluorooctane sulfonic acid and perfluorooctanoic acid in food[J]. EFSA Journal, 2018, 16（12）: e05194.
[16] SCHRENK D, BIGNAMI M, BODIN L, et al. Risk to human health related to the presence of perfluoroalkyl substances in food[J]. EFSA Journal, 2020, 18（9）: e06223.
[17] 张文, 闫君, 陈婷, 等. UPLC-MS/MS法测定食用植物油中15种全氟化合物[J]. 化学试剂, 2022, 44（3）: 455-461. ZHANG W, YAN J, CHEN T, et al. Determination of 15 perfluorinated compounds in edible vegetable oil by UPLC-MS/MS[J]. Chemical Reagents, 2022, 44（3）: 455-461.
[18] 张文, 陈婷, 闫君, 等. 改进的QuEChERS-UPLC-MS/MS法测定茶叶中15种全氟化合物[J]. 分析试验室, 2021, 40（11）: 1 314-1 319. ZHANG W, CHEN T, YAN J, et al. A modified QuEChERS-UPLC-MS/MS method for the determination of 15 perfluorinated compounds in tea[J]. Chinese Journal of Analysis Laboratory, 2021, 40（11）: 1 314-1 319.
[19] 孙小航, 朱丽, 宁凡盛, 等. 食用农产品中全氟化合物检测技术的研究进展[J]. 食品研究与开发, 2019, 40（7）: 213-217. SUN X H, ZHU L, NING F S, et al. Progress on detection technology of perfluorinated compounds in edible agricultural products[J]. Food Research and Development, 2019, 40（7）: 213-217.
[20] 李静, 张鸿, 柴之芳, 等. 分散固相萃取结合HPLC-MS/MS检测鸡蛋中16种全氟化合物[J]. 分析测试学报, 2014, 33（10）: 1 109-1 115. LI J, ZHANG H, CHAI Z F, et al. Determination of sixteen perfluorinated compounds in egg by high performance liquid chromatography- tandem mass spectrometry combined with dispersive solid phase extraction[J]. Journal of Instrumental Analysis, 2014, 33（10）: 1 109-1 115.
[21] 王莹, 杜思宇, 张红, 等. 改进的QuEChERS-UPLC-MS/MS法测定动物源性食品中13种全氟化合物[J]. 食品工业科技, 2021, 42（1）: 239-249. WANG Y, DU S Y, ZHANG H, et al. Determination of thirteen PFCs in animal-derived food by improved QuEChERS extraction coupled with ultra-high performance liquid chromatography-tandem mass spectrometry[J].Science and Technology of Food Industry, 2021, 42（1）: 239-249.
[22] 李帅, 陈辉, 金铃和, 等. 高效液相色谱—串联质谱法测定蜂蜜中20种全氟烷基化合物[J]. 色谱, 2017, 35（5）: 495-501. LI S, CHEN H, JIN L H, et al. Determination of 20 perfluorinated alkyl substances in honey by high performance liquid chromatography-tandem mass spectrometry[J].Chinese Journal of Chromatography, 2017, 35（5）: 495-501.
[23] 薛荣旋, 卢丽明, 黄诚, 等. QuEChERS-超高效液相色谱—串联质谱法测定水产品中6种氟喹诺酮类抗生素残留量[J]. 中国食品卫生杂志, 2020, 32（5）: 519-523. XUE R X, LU L M, HUANG C, et al. Determination of six fluoroquinolones in aquatic products by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry[J].Chinese Journal of Food Hygiene, 2020, 32（5）: 519-523.
[24] 国家质量监督检验检疫总局. 实验室质量控制规范 食品理化检测: GB/T 27404—2008[S/OL]. （2008-05-04） [2022-12-09]. http://down.foodmate.net/standard/yulan.php?itemid=15544. General Adminitration of Quality Supervision, Inspection and Quarantine of the People's Republic of China. Criterion on quality control of laboratoria—Chemical testing of food: GB/T 27404—2008[S/OL]. （2008-05-04） [2022-12-09]. http://down.foodmate.net/standard/yulan.php?itemid=15544.