Determination of three sterols in camellia oleifera seed oil by atmospheric pressure chemical ionization ultra performance liquid chromatography-time of flight mass spectrometry

Authors

PAN Jin-hua, Guangzhou Inspection and Testing Certification Group Co., Guangzhou, Guangdong 511447, China;National Quality Testing Center for Processed Food 〔Guangdong〕, Guangzhou, Guangdong 511447, ChinaFollow
CHEN Feng, Guangzhou Inspection and Testing Certification Group Co., Guangzhou, Guangdong 511447, China;National Quality Testing Center for Processed Food 〔Guangdong〕, Guangzhou, Guangdong 511447, China
BAI Jian-ping, Guangzhou Inspection and Testing Certification Group Co., Guangzhou, Guangdong 511447, China;National Quality Testing Center for Processed Food 〔Guangdong〕, Guangzhou, Guangdong 511447, China
DUAN Xue-fei, Guangzhou Inspection and Testing Certification Group Co., Guangzhou, Guangdong 511447, China;National Quality Testing Center for Processed Food 〔Guangdong〕, Guangzhou, Guangdong 511447, China
LI Qi-qi, Guangzhou Inspection and Testing Certification Group Co., Guangzhou, Guangdong 511447, China;National Quality Testing Center for Processed Food 〔Guangdong〕, Guangzhou, Guangdong 511447, China

Corresponding Author(s)

潘锦华（1995—），男，广州检验检测认证集团有限公司助理工程师，学士。E-mail: 1369800445@qq.com

Abstract

Objective: In this study, ultra performance liquid chromatography-high resolution quadrupole time of flight mass spectrometer (UPLC-Q-TOF/MS) was established to determine β-sitosterol, brassicasterol, and campesterol in camellia oleifera seed oil. Methods: Using 6-ketocholestanol as the internal standard and performing sample extraction by heating it for 30 min and refluxing it with 50 mL ethanol. After centrifugation and layering, three sterols were separated by Xbridge BEH C₁₈ (100 mm × 4.6 mm, 2.5 μm) Chromatographic column elution. Finally, ionized by atmospheric pressure chemical ionization source and analyzed by positive ion mode. Results: The internal standard curves of the three sterols showed an observable linear relationship in the range of 0.1～10.0 mg/L, while the correlation coefficient R² was both greater than 0.999 2. The average recoveries at 0.5, 1.0, 2.0, and 10.0 times of background value were in the range of 84.5%～99.5%. The relative standard deviation (RSDs) was ranged from 3.92% to 7.44%. Moreover, the limits of detection were 1.0 mg/kg and the limits of quantitation were 3.0 mg/kg, respectively. Conclusion: The method was simple in operation, low in solvent consumption, and toxicity. It had good recoveries and reproducibility that could be applied for the accurate determination of three sterols in camellia oleifera seed oil.

Publication Date

10-20-2023

First Page

56

Last Page

61

DOI

10.13652/j.spjx.1003.5788.2022.81228

Recommended Citation

Jin-hua, PAN; Feng, CHEN; Jian-ping, BAI; Xue-fei, DUAN; and Qi-qi, LI (2023) "Determination of three sterols in camellia oleifera seed oil by atmospheric pressure chemical ionization ultra performance liquid chromatography-time of flight mass spectrometry," Food and Machinery: Vol. 39: Iss. 7, Article 8.
DOI: 10.13652/j.spjx.1003.5788.2022.81228
Available at: https://www.ifoodmm.cn/journal/vol39/iss7/8

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