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Abstract

Objective: This study aimed to investigate the effect of Eurotium Cristatum fermentation broth mixed with tea polyphenols on the immune indexes of cyclophosphamide immunosuppressive mice. Methods: Immunosuppressive model was established by intraperitoneal injection of cyclophosphamide with 80 mg/kg for 3 days. High, middle and low dose E. Cristatum fermentation broth mixed with tea polyphenols was administrated to immunosuppressive mice intragastrically for 15 days. The blood index, spleen index, macrophage phagocytic index, half hemolysis value, delayed-type hypersensitivity, the content of IL-2 and tumor necrosis factor-α (TNF-α) were measured. Results: E. Cristatum fermentation broth mixed with tea polyphenols significantly increased the number of white blood cells, red blood cells, hemoglobin, serum hemolysin and IL-2 on immunosuppressive mice, enhanced the spleen index and monocyte-phagocytic index, and improved the delayed type hypersensitivity on mice. Conclusion: E. Cristatum fermentation broth mixed with tea polyphenols can enhance immunity on mice.

Publication Date

10-20-2023

First Page

160

Last Page

165

DOI

10.13652/j.spjx.1003.5788.2022.80782

References

[1] ZAHRA A, NEGAR S, FARNAZ Z, et al. Human neutrophil peptide 1 as immunotherapeutic agent against leishmania infected BALB/c mice[J]. PLoS Neglected Tropical Diseases, 2017, 11（12）: e0006123.
[2] XU X G, HU J F, MA J X, et al. Essential roles of TIM-1 and TIM-4 homologs in adaptive humoral immunity in a Zebrafish model[J]. Journal of Immunology, 2016, 196（4）: 1 686-1 699.
[3] SEGURA E. Cross-presentation assay for human dendritic cells[J]. Methods in Molecular Biology, 2016, 1 423（2）: 189-198.
[4] CHEN M, ZHANG F, SU Y, et al. Immunomodulatory effects of egg white peptides on immunosuppressed mice and sequence identification of immunomodulatory peptides[J]. Food Bioscience, 2022, 49: 101873.
[5] XIAO Y, ZHONG K, BAI J, et al. The biochemical characteristics of a novel fermented loose tea by Eurotium cristatum （MF800948） and its hypolipidemic activity in a zebrafish model[J]. LWT-Food Science and Technology, 2020, 117: 108629.
[6] 吴香兰, 刘仲华, 曹丹, 等. 茯砖茶对小鼠肠道免疫功能调节作用的研究[J]. 茶叶科学, 2013, 33（2）: 125-130. WU X L, LIU Z H, CAO D, et al. Effect of Fuzhuan brick tea on the adjustment of intestinal immune function in mice[J]. Journal of Tea Science, 2013, 33（2）: 125-130.
[7] 刘平, 李宗军, 许爱清. 茯砖茶水提物对大肠杆菌感染小鼠的免疫调节作用[J]. 湖南农业大学学报（自然科学版）, 2011, 37（5）: 537-539, 566. LIU P, LI Z J, XU A Q. Immunological effect of water extract from fuzhuan brick-tea on mice infected with Escherichia coli[J]. Journal of Hunan Agricultural University（Natural Sciences）, 2011, 37（5）: 537-539, 566.
[8] 赵航晔, 夏琛, 何普明, 等. 茶多酚抗炎和促外伤愈合作用及其机制[J]. 浙江大学学报（农业与生命科学版）, 2021, 47（1）: 118-126. ZHAO H Y, XIA C, HE P M, et al. Effects of tea polyphenols on anti-inflammation and promotion of wound healing and its mechanisms[J]. Journal of Zhejiang University （Agriculture and Life Sciences）, 2021, 47（1）: 118-126.
[9] 彭颖. 冠突散囊菌发酵液对免疫抑制小鼠免疫学指标的影响[J]. 食品与机械, 2021, 37（8）: 169-173. PENG Y. The effects of Eurotium Cristatum fermentation broth on immunological indicators in immunosuppressive mice[J]. Food & Machinery, 2021, 37（8）: 169-173.
[10] 申开泽, 万庆家, 熊宇, 等. 芦荟复配茶多酚和维C对小鼠免疫力的影响[J]. 农产品加工, 2022（8）: 12-14, 18. SHEN K Z, WAN Q J, XIONG Y, et al. Effect on immunity function of aloe vera abstract mixed with tea polyphenols and vitamin C in mice[J]. Farm Products Processing, 2022（8）: 12-14, 18.
[11] KIM J W, CHOI J S, SEOL D J, et al. Immunomodulatory effects of kuseonwangdogo-based mixed herbal formula extracts on a cyclophosphamide-induced immunosuppression mouse model[J]. Evidence-Based Complementray and Alternative Medicine, 2018, 2 018: 1-14.
[12] 陈汀, 成旭东, 胡芳, 等. 扶正胶囊提取物对环磷酰胺诱导的免疫低下小鼠免疫调节作用研究[J].天津中医药大学学报, 2022, 41（4）: 451-456. CHEN T, CHENG X D, HU F, et al. Study on immunomodulatory effect of fuzheng capsule extract on immunocompromised mice induced by cyclophosphamide[J]. Journal of Tianjin University of Traditional Chinese Medicine, 2022, 41（4）: 451-456.
[13] 赵雯, 刘伟贤, 张海斌, 等. 乳双歧杆菌BL-99以及副干酪乳酪杆菌ET-22增强小鼠免疫功能的研究[J]. 中国乳品工业, 2021, 49（11）: 13-18. ZHAO W, LIU W X, ZHANG H B, et al. Effects of Bifidobacterium Lactis BL-99 and Lactobacillus Paracasei （Lacticasei Bacillus Paracasei） ET-22 on regulating immune function in mice[J]. China Dairy Industry, 2021, 49（11）: 13-18.
[14] 李敏, 王劼, 赵谋明. 灵芝、姬松茸、黄芪混合多糖配伍茶多酚复合剂免疫增强作用的研究[J]. 现代食品科技, 2009, 25（8）: 885-888, 880. LI M, WANG J, ZHAO M M. Effects of a compound agent of ganoderma lucidum, agaricus blazei, tea polyphenols and astragulus on immune enhancement of mice[J]. Modern Food Science and Technology, 2009, 25（8）: 885-888, 880.
[15] 张敏佳, 刘文颖, 贾福怀, 等. 豌豆肽对环磷酰胺致免疫抑制小鼠免疫功能的影响[J]. 食品与发酵工业, 2018, 44（8）: 135-140. ZHANG M J, LIU W H, JIA F H, et al. Effect of pea peptide on immune function in immunosuppressed mice induced by cyclophosphamide[J]. Food and Fermentation Industries, 2018, 44（8）: 135-140.
[16] 肖红艳, 屈金涛, 凌浩, 等. 茶多酚对奶山羊生产性能、血液指标和抗氧化功能的影响[J]. 动物营养学报, 2021, 33（8）: 4 533-4 540. XIAO H Y, QU J T, LIN H, et al. Effects of tea polyphenols on performance, blood indexes and antioxidant function of dairy goats[J]. Chinese Journal of Animal Nutrition, 2021, 33（8）: 4 533-4 540.
[17] 赵培培, 禚映辰, 赵雪, 等. 环磷酰胺在节律性化疗中的研究进展[J]. 中国医院药学杂志, 2018, 38（1）: 104-108. ZHAO P P, ZHUO Y C, ZHAO X, et al. Research progress of cyclophosphamide in metronomic chemotherapy[J]. Chinese Journal of Hospital Pharmacy, 2018, 38（1）: 104-108.
[18] 徐光沛, 蒋平, 何燕飞, 等. 构树叶粗多糖对环磷酰胺诱导免疫抑制小鼠免疫功能的影响[J]. 西北农业学报, 2022（7）: 1-8. XU G P, JIANG P, HE Y F, et al. Effects of crude polysaccharide from leaves of a tree on immune function in immunosuppressive mice induced by cyclophosphamide[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2022（7）: 1-8.
[19] 李波. 香菇多糖和黄芪多糖对小鼠免疫平衡影响的研究[D]. 长沙: 湖南农业大学, 2011: 22-24. LI B. The lentinan and astragalus polysaccharides influenced the immunologic balance in mice model[D]. Changsha: Hunan Agricultural University, 2011: 22-24.
[20] 龚蕾, 郎茜, 马莎, 等. 单次大剂量和多次小剂量环磷酰胺腹腔注射对小鼠免疫抑制模型的影响[J]. 中国畜牧兽医, 2021, 48（10）: 3 787-3 794. GONG L, LANG X, MA S, et al. Effect of single high dose and multiple low dose cyclophosphamide abdominal injection on immunosuppressive model in mice[J]. China Animal Husbandry & Veterinary Medicine, 2021, 48（10）: 3 787-3 794.
[21] 郭春宏, 李正翔. 茶多酚免疫药理作用研究[J]. 天津医科大学学报, 2009, 15（1）: 102-104. GUO C H, LI Z X. Study of the immunopharmacological effects of teapolyphenols[J]. Journal of Tianjin Medical University, 2009, 15（1）: 102-104.
[22] 范冬月, 李倩, 邹先伟, 等. 冠突散囊菌与木霉混合发酵液醇提物对小鼠巨噬细胞RAW264.7免疫调节的影响[J]. 中国酿造, 2021, 40（5）: 37-42. FAN D Y, LI Q, ZOU X W, et al. Effect of ethanol extracts from co-fermentation liquid of Eurotiunm cristatum and Trichoderma sp. on immunoregulation of mouse macrophages RAW264.7[J]. China Brewing, 2021, 40（5）: 37-42.
[23] 谷仿丽, 黄仁术, 刘玉曼. 金荞麦多糖对环磷酰胺致免疫低下小鼠免疫功能的影响[J]. 中药材, 2015, 38（2）: 370-372. GU F L, HUANG R H, LIU Y M. Effect of buckwheat polysaccharide on immune function of immunocompromised mice induced by cyclophosphamide[J]. Journal of Chinese Medicinal Materials, 2015, 38（2）: 370-372.
[24] 郑厚胜, 郑斯文, 王英平, 等. 人参皂苷Rg3对环磷酰胺致免疫功能低下小鼠的免疫调节作用[J].中成药, 2021, 43（11）: 3 202-3 206. ZHEN H S, ZHEN S W, WANG Y P, et al. Immunomodulatory effect of ginsenoside Rg3 on immunocompromised mice induced by cyclophosphamide[J]. Chinese Traditional Patent Medicine, 2021, 43（11）: 3 202-3 206.
[25] 徐艳明, 黄壹敏, 石杰, 等. 冠突散囊菌来源的2-6-H-5-B化合物的抗炎作用及机制研究[J]. 中国现代应用药学, 2022, 39（2）: 161-167. XU Y M, HUANG Y M, SHI J, et al. Study on anti-inflammatory activity and mechanism of compound 2-6-H-5-B from Eurotium cristatum[J]. Chinese Journal of Modern Applied Pharmacy, 2022, 39（2）: 161-167.
[26] 乔石, 闵思明, 胡惠宇, 等. 太子参参须多糖对免疫抑制小鼠的保护作用[J]. 福建农林大学学报（自然科学版）, 2022, 51（5）: 697-704. QIAO S, MIN S M, HU H Y, et al. Protective effect of radix pseudostellariae fibrous root polysaccharide on immunosuppressive mice[J]. Journal of Fujian Agriculture and Forestry University （Natural Science Edition）, 2022, 51（5）: 697-704.

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