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Authors

LU Huan, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;Key Laboratory of Applied Fungi Resources and Utilization 〔South〕, Ministry of Agriculture, P. R. China, Shanghai 201403, China;National Engineering Research Center of Edible Fungi, Shanghai 201403, China;Shanghai Key Laboratory of Agriculture Genetics and Breeding, Shanghai 201403, China
LIU Jianyu, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;Key Laboratory of Applied Fungi Resources and Utilization 〔South〕, Ministry of Agriculture, P. R. China, Shanghai 201403, China;National Engineering Research Center of Edible Fungi, Shanghai 201403, China;Shanghai Key Laboratory of Agriculture Genetics and Breeding, Shanghai 201403, China
SHANG Xiaodong, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;Key Laboratory of Applied Fungi Resources and Utilization 〔South〕, Ministry of Agriculture, P. R. China, Shanghai 201403, China;National Engineering Research Center of Edible Fungi, Shanghai 201403, China;Shanghai Key Laboratory of Agriculture Genetics and Breeding, Shanghai 201403, China
YANG Hui, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;Key Laboratory of Applied Fungi Resources and Utilization 〔South〕, Ministry of Agriculture, P. R. China, Shanghai 201403, China;National Engineering Research Center of Edible Fungi, Shanghai 201403, China;Shanghai Key Laboratory of Agriculture Genetics and Breeding, Shanghai 201403, China
WU Yansha, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;Key Laboratory of Applied Fungi Resources and Utilization 〔South〕, Ministry of Agriculture, P. R. China, Shanghai 201403, China;National Engineering Research Center of Edible Fungi, Shanghai 201403, China;Shanghai Key Laboratory of Agriculture Genetics and Breeding, Shanghai 201403, China
WANG Ruijuan, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;Key Laboratory of Applied Fungi Resources and Utilization 〔South〕, Ministry of Agriculture, P. R. China, Shanghai 201403, China;National Engineering Research Center of Edible Fungi, Shanghai 201403, China;Shanghai Key Laboratory of Agriculture Genetics and Breeding, Shanghai 201403, ChinaFollow

Corresponding Author(s)

王瑞娟(1980—),女,上海市农业科学院副研究员,硕士。E-mail:wangruijuan @saas.sh.cn

Abstract

Objective: This study aimed to investigate the quality differences among different hybrid enoki mushrooms (Flammulina filiformis) strains and breed new strains with high quality. Methods: Two different protoplast monocytes of yellow enoki mushrooms Fv1923 were used as one of the parents to cross with 6 enoki mushrooms protoplasts (2 yellow and 4 white strains), and a total of 12 yellow hybrid strains were obtained. Principal component analysis and cluster analysis were used to evaluate the free amino acids of 12 hybrid strains, and the flavor diversity of different protoplast hybrid strains was also evaluated. Results: The average contents of essential amino acids, non-essential amino acids and total amino acids were 7.71, 22.83 and 30.54 mg/g, respectively. Methionine was the first limiting amino acid of strains F1, F11, F15, F16, F21, F29, F39, F41 and F42. The amino acid components in 12 hybrid strains had a strong correlation, and the cumulative variance contribution rate of the four principal components was 87.15%, which better reflected the comprehensive information of amino acids in hybrid strains. The top two strains in comprehensive scores were hybrid strains of Shangyan 1820 and Fv1923. Cluster analysis showed that 12 strains were divided into 6 groups, which was consistent with the results of principal component analysis and reflected the variety differences of free amino acids in different strains. Conclusion: There were significant differences in the composition and content of free amino acids inhybrid strains with different protoplast monocytes as parents.

Publication Date

10-20-2023

First Page

25

Last Page

33,41

DOI

10.13652/j.spjx.1003.5788.2023.80237

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