Abstract
Objective: To establish a HPLC method for simultaneous determination of rutin, hyperoside, quercetin in Xiang lotus. Methods: The HPLC method was performed on an Agilent ZORBAX SB-C18 column (4.6 mm×250 mm, 5 m) with gradient elution of methanol and 0.6% phosphoric acid solution. The flow rate was 1.0 mL/min. The column temperature was 30 ℃ and the detection wavelength was set at 255 nm. Results: The contents of rutin, hypericin and quercetin in 20 batches of lotus samples were 1.32～2.06, 1.36～2.43, 1.41～3.26 mg/kg, respectively. The linear range of the concentration of rutin, hypericin and quercetin was 5～200 g/mL (R2=0.999 9), the three flavonoids showed a good linearity, a high sensitivity, and good serviceability. The relative standard deviation (RSD) for precision, stability, repeatability, and recoveries test were all less than 4.0%, and the average recovery rate of added standard were 96.6%, 96.9%, 97.4%, respecitvely. Conclusion: The method is convenient and accurate, and can be used for the content determination and quality evaluation of flavonoids in Xiang lotus.
Publication Date
10-30-2023
First Page
57
Last Page
64
DOI
10.13652/j.spjx.1003.5788.2023.80595
Recommended Citation
Feifei, ZHONG; Jing, LI; Deqing, LEI; Yun, YANG; Jianfang, WU; and Li, OUYANG
(2023)
"Determination of rutin, hyperoside, quercetin in Xiang lotus by HPLC,"
Food and Machinery: Vol. 39:
Iss.
9, Article 9.
DOI: 10.13652/j.spjx.1003.5788.2023.80595
Available at:
https://www.ifoodmm.cn/journal/vol39/iss9/9
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