Abstract
Objective: With the Polygonatum sibiricum as raw material, to develop a process for the combined extraction of Polygonatum sibiricum sponins (PSSs) and polysaccharides (PSPs), and the inhibitory activities of the extract and their complexes (PSSs-PSPs) on α-glucosidase and α-amylase were investigated. Methods: PSSs and PSPs were performed using ultrasound combined with enzymatic hydrolysis. The effects of solid-liquid ratio, ultrasonic time, and ultrasonic temperature on the yield of PSSs, the types of multi-enzymes, the proportion of complex enzymes, enzymolysis time, enzyme dosage, solid-liquid ratio, hot water extraction time, and hot water extraction temperature on the yield of PSPs were investigated, and the Box-Behnken experiment design was used to optimize the extraction of PSSs and PSPs. Results: The optimum extraction parameters of PSSs and PSPs were as follows: ultrasonic temperature was 50 ℃, ultrasonic time was 50 min, ultrasonic solid-liquid ratio was 1∶25 (g/mL), enzymolysis time was 2 h, enzyme dosage was 3 053 U/g, solid-liquid ratio was 1∶25 (g/mL), hot water extraction temperature was 80 ℃, and hot water extraction time was 1 h. Under the control of these conditions, the total yield of PSSs and PSPs was 12.22% and the 27.07%. The inhibitory activities of the extract and their complexes (PSSs-PSPs) on α-glucosidase and α-amylase were verified, and their complexes also have a good inhibitory effect on those enzymes. Conclusion: The process conditions for the efficient synergistic extraction of polygonatin and polysaccharide were obtained after optimization. Compared with a single component, the hypoglycemic activity of their complexes was improved.
Publication Date
1-30-2024
First Page
158
Last Page
166,196
DOI
10.13652/j.spjx.1003.5788.2023.80404
Recommended Citation
Bingzong, XIE; Mi, LI; Caiwen, DONG; Xin, WANG; Han, LIU; and Yanli, MA
(2024)
"Study on the technology of ultrasonic assisted combined extraction of saponins and polysaccharides from Polygonatum sibiricum and its hypoglycemic activity,"
Food and Machinery: Vol. 40:
Iss.
1, Article 24.
DOI: 10.13652/j.spjx.1003.5788.2023.80404
Available at:
https://www.ifoodmm.cn/journal/vol40/iss1/24
References
[1] 国家药典委员会. 中华人民共和国药典: 一部[S]. 2020版. 北京: 中国医药科技出版社, 2020: 319.
National Pharmacopoeia Commission. Pharmacopoeia of the people's republic of China: Volume 1[S]. 2020 Edition. Beijing: China Medical Science and Technology Press, 2020: 319.
[2] 于纯淼, 刘宁, 宫铭海, 等. 黄精药理作用研究进展及在保健食品领域的应用开发[J]. 黑龙江科学, 2019, 10（18）: 66-68.
YU C M, LIU N, GONG M H, et al. Research progress and application development status of pharmacology of Polygonatum sibiricum[J]. Heilongjiang Science, 2019, 10（18）: 66-68.
[3] WANG Q, BAN J, CAI R, et al. Metabolic composition and quality traits of Polygonatum cyrtonema Hua from different germplasms and age sections based on widely targeted metabolomics analysis[J]. International Journal of Molecular Sciences, 2023, 24（7）: 6 077.
[4] 张娜娜, 潘慧云, 侯芯芯, 等. 黄精研究现状与可持续发展探析[J]. 安徽农学通报, 2022, 28（10）: 24-26.
ZHANG N N, PAN H Y, HOU X X, et al. Analysis on the present situation and sustainable development of Polygonatum[J]. Anhui Agricultural Bulletin, 2022, 28（10）: 24-26.
[5] GAO Q H, FU X, ZHANG R, et al. Neuroprotective effects of plant polysaccharides:A review of the mechanisms[J]. International Journal of Biological Macromolecules, 2018, 106: 749-754.
[6] XIE S Z, ZHANG W J, LIU W, et al. Physicochemical characterization and hypoglycemic potential of a novel polysaccharide from Polygonatum sibiricum Red through PI3K/Akt mediated signaling pathway[J]. Journal of Functional Foods, 2022, 93: 105080.
[7] LI L, THAKUR K, LIAO B Y, et al. Antioxidant and antimicrobial potential of polysaccharides sequentially extracted from Polygonatum cyrtonema Hua[J]. International Journal of Biological Macromolecules, 2018, 114: 317-323.
[8] ZHENG S. Protective effect of Polygonatum sibiricum polysaccharide on D-galactose-induced aging rats model[J]. Scientific Reports, 2020, 10（1）: 2 246.
[9] WANG J, LU C S, LIU D Y, et al. Constituents from Polygonatum sibiricum and their inhibitions on the formation of advanced glycosylation end products[J]. Journal of Asian Natural Products Research, 2016, 18（7）: 697-704.
[10] CHOI S B, PARK S. A steroidal glycoside from Polygonatum odoratum （Mill.） Druce. improves insulin resistance but does not alter insulin secretion in 90% pancreatectomized rats[J]. Bioscience, Biotechnology, and Biochemistry, 2002, 66（10）: 2 036-2 043.
[11] MA Y L, ZHANG Y S, ZHANG F, et al. Methylprotodioscin from Polygonatum sibiricum inhibits cervical cancer through cell cycle arrest and apoptosis induction[J]. Food and Chemical Toxicology, 2019, 132: 110655.
[12] ZHAO P, LI X, WANG Y, et al. Comparative studies on characterization, saccharide mapping and antiglycation activity of polysaccharides from different Polygonatum ssp[J]. Journal of Pharmaceutical and Biomedical Analysis, 2020, 186: 113243.
[13] 金昊艺, 林洪宇, 赵东夫, 等. 响应面法优化黄精多糖提取工艺[J]. 食品安全导刊, 2022（20）: 148-150, 154.
JIN H Y, LIN H Y, ZHAO D F, et al. Optimization of the extraction process of Polygonatum polysaccharide by response surface methodology[J]. Journal of Food Safety, 2022（20）: 148-150, 154.
[14] 王毅, 穆麒麟, 田小海, 等. 正交法优化黄精多糖的提取工艺[J]. 湖北农业科学, 2020, 59（15）: 114-117.
WANG Y, MU Q L, TIAN X H, et al. Optimization of extraction technology of polysaccharide in Polygonatum sibiricum by orthogonal method[J]. Hubei Agricultural Science, 2020, 59（15）: 114-117.
[15] 赵丽蓉, 罗汉, 相英龙, 等. Box-Benhken响应面法优化微波提取黄精总皂苷的条件[J]. 中国现代中药, 2018, 20（8）: 1 010-1 015.
ZHAO L R,LUO H, XIANG Y L, et al. Optimization of microwave extracting total saponins from Rhizoma Polygonti by Box-Benhken response surface method[J]. Modern Chinese Medicine, 2018, 20（8）: 1 010-1 015.
[16] 庞红霞, 崔婧, 范桂强, 等. 黄精皂苷提取条件的Design-Expert优化及其降血糖效果初步研究[J]. 中国药师, 2018, 21（9）: 27-30, 42.
PANG H X, CUI J, FAN G Q, et al. Extraction optimization of saponin from Polygonatum sibiricum by Design-Expert and preliminary study on its hypoglycemic effect[J]. Chinese Pharmacology, 2018, 21（9）: 27-30, 42.
[17] 马艳平, 张叶琪, 张婧娴, 等. 闪式提取法在中药复方制剂中的研究进展[J]. 中医药导报, 2020, 26（5）: 106-109.
MA Y P, ZHANG Y Q, ZHANG J X, et al. Research progress of flash extraction method on compound Chinese medicine prescriptions[J]. Introduction to Traditional Chinese Medicine, 2020, 26（5）: 106-109.
[18] 陈蕾, 万婉, 刘艳, 等. 黄精有效成分提取测定的研究[J]. 赤峰学院学报（自然科学版）, 2019, 35（9）: 37-39.
CHEN L, WAN W, LIU Y, et al. Study on the extraction and determination of effective components in Polygonatum[J]. Journal of Chifeng University （Natural Science Edition）, 2019, 35（9）: 37-39.
[19] 苑璐, 冷凯良, 周余航, 等. 复合酶解法优化黄精多糖提取工艺[J]. 食品与生物技术学报, 2017, 36（9）: 996-1 001.
YUAN L, LENG K L, ZHOU Y H, et al. Optimization of multi-enzymatic extraction process of polysaccharide from Polygonatum sibiricum[J]. Journal of Food and Biotechnology, 2017, 36（9）: 996-1 001.
[20] 张梓原, 徐伟, 王鑫, 等. 黄精多糖的提取工艺对比研究[J]. 包装工程, 2020, 41（9）: 51-58.
ZHANG Z Y, XU W, WANG X, et al. Comparative study on extraction technologies of polysaccharides from Rhizoma Polygonatum[J]. Packaging Engineering, 2020, 41（9）: 51-58.
[21] 刘勇, 张彩虹, 谢普军, 等. 响应面优化皂角刺皂苷超声波辅助提取工艺及其降血糖活性[J]. 林产化学与工业, 2023, 43（2）: 12-18.
LIU Y, ZHANG C H, XIE P J, et al. Optimization of ultrasonic-assisted extraction technology of saponins from Gleditsia sinensis Thorn by RSM and its hypoglycemic activity[J]. Chemistry and Industry of Forest Products, 2023, 43（2）: 12-18.
[22] 李云姣, 李琪, 杜佳峰, 等. 水果酵素体外抗氧化及抑制α-淀粉酶和α-葡萄糖苷酶活性的研究[J]. 中国食品学报, 2019, 19（4）: 79-84.
LI Y J, LI Q, DU J F, et al. Antioxidant activity and inhibitory effects on α-amylase and α-glucosidase of fermented fruit juice in vitro[J]. Journal of Chinese Food Science, 2019, 19（4）: 79-84.
[23] 徐丽梅, 陈炼红. 响应面复合酶法提取紫薯多糖及抗氧化活性研究[J]. 中国调味品, 2022, 47（4）: 190-197.
XU L M, CHEN L H. Study on the extraction of polysaccharides from Purple Sweet Potato by response surface and compound enzymemethods and the antioxidant activity[J]. Chinese Seasoning, 2022, 47（4）: 190-197.
[24] 任昌娟, 朱云龙. 生姜多糖提取技术工艺优化及效益分析[J]. 中国调味品, 2021, 46（9）: 117-120.
REN C J, ZHU Y L. Extraction technology optimization and benefit analysis of Ginger polysaccharides[J]. Chinese Seasonings, 2021, 46（9）: 117-120.
[25] 陈永, 宋真萍, 吴晖, 等. 红蓝草多糖提取工艺优化及其抗氧化活性分析[J]. 食品工业科技, 2023, 44（5）: 166-173.
CHEN Y, SONG Z P, WU H, et al. Optimization of the extraction process of Peristrophe roxburghiana polysaccharide and analysis of its antioxidant activity[J]. Food Industry Technology, 2023, 44（5）: 166-173.
[26] 方如银, 李铭德. 复合酶法优化黄精多糖的提取工艺研究[J]. 中国处方药, 2018, 16（7）: 32-33.
FANG R Y, LI M D. Optimization of enzymatic extraction of polysaccharides from Polygonati Rhizoma with mixed enzymes[J]. Chinese Prescription Medicine, 2018, 16（7）: 32-33.
[27] 包瑞敏, 张智, 杜亚飞, 等. 黄精总皂苷提取工艺优化及其对α-淀粉酶及α-葡萄糖苷酶抑制活性[J]. 食品工业科技, 2020, 41（16）: 163-168, 175.
BAO R M, ZHANG Z, DU Y F, et al. Optimization of extraction technology of total saponin from Polygonatum Sibiricum and its inhibitory activity to α-amylase and α-glucosidase[J]. Food Industry Technology, 2020, 41（16）: 163-168, 175.
[28] 黄祥元, 黄美容. 黄精提取物中总皂苷含量的测定[J]. 食品与机械, 2012, 28（6）: 106-108.
HUANG X Y, HUANG M R. Content determination of total saponins in extracts of Rhizoma Polygonati[J]. Food & Machinery, 2012, 28（6）: 106-108.
[29] 喻祖文, 张旺凡. 多倍体黄精中多糖和皂苷的提取及含量测定[J]. 中国现代中药, 2011, 13（5）: 20-22.
YU Z W, ZHANG W F. The extraction and determination of polyploid main active ingredients of Polygonatum[J]. Modern Chinese Medicine, 2011, 13（5）: 20-22.
[30] YOU X J, GUO R, WANG L, et al. Ultrasonic extraction process of saponins from Polygonatum sibiricum[J]. Journal of Northwest Foresty University, 2010, 25（3）: 163-166.
[31] LIU G, FENG S, SUI M, et al. Extraction and identification of steroidal saponins from Polygonatum cyrtonema Hua using natural deep eutectic solvent-synergistic quartz sand-assisted extraction method[J]. Journal of Separation Science, 2023, 46（7）: 2200823.