Abstract
［Objective］ To investigate the antibacterial effect of ε-polysine hydrochloride on Shewanella baltica and Pseudomonas fluorescen s derived from large yellow croaker.［Methods］ The antibacterial effects of ε-polylysine hydrochloride on the growth of Shewanella baltica and Pseudomonas fluorescens was analyzed by the minimum inhibitory concentration and the growth curve.By measuring the bacterial fluid conductivity,alkaline phosphatase activity,ultraviolet absorbent substances and biofilm formation inhibition rate,the cell integrity and permeability were evaluated.The aerobic plate count and total volatile basic nitrogen of the flesh of large yellow croaker inoculated with spoilage bacteria were determined the effect of ε-polylysine hydrochloride on the spoilage ability of Shewanella baltica and Pseudomonas fluorescens.［Results］ The minimum inhibitory concentrations of ε-polylysine hydrochloride against Shewanella baltica was 0.250 mg/mL,while Pseudomonas fluorescein was 0.125 mg/mL.With the treatment of ε-polylysine hydrochloride,the growth of Shewanella baltica and Pseudomonas fluorescein was significantly inhibited,and the conductivity,AKP enzyme activity and UV absorbent leakage were significantly increased in a mass concentration dependent manner.Fish inoculated with Shewanella baltica and Pseudomonas fluorescein showed a significant decrease in total bacterial count and TVB -N values.［Conclusion］ ε-polylysine hydrochloride can destroy the cell integrity of Shewanella baltica and Pseudomonas fluorescein,inhibit the growth and affect the spoilage ability.
Publication Date
4-3-2025
First Page
133
Last Page
139
DOI
10.13652/j.spjx.1003.5788.2024.80550
Recommended Citation
Weibin, ZHANG; Shuai, CHEN; Shuangni, SHI; and Zhiqiu, YE
(2025)
"The antibacterial effects of ε-polylysine hydrochloride against predominant spoilage bacteria from large yellow croaker,"
Food and Machinery: Vol. 41:
Iss.
1, Article 17.
DOI: 10.13652/j.spjx.1003.5788.2024.80550
Available at:
https://www.ifoodmm.cn/journal/vol41/iss1/17
References
[1] 农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产学会.2023 中国渔业统计年鉴 [R].北京:中国农业出版社,2023:22.Ministry of Agriculture and Rural Affairs of Fisheries and Fishery Administration,National Fisheries Technology Extension Station,China Society of Fisheries.2023 China fishery statistical yearbook [R].Beijing:China Agriculture Press,2023:22.
[2] 李学英,许钟,郭全友,等.大黄鱼冷藏过程中的鲜度变化 [J].中国水产科学,2009,16(3):442-450.LI X Y,XU Z,GUO Q Y,et al.Changes of freshness in cultured Pseudosciaena crocea during chilled storage [J].Journal of Fishery Sciences of China,2009,16(3):442-450.
[3] 张雯,倪莉,黄志清,等.冰鲜大黄鱼肠腔细菌类群鉴定及菌群结构分析 [J].中国食品学报,2013,13(12):188-196.ZHANG W,NI L,HUANG Z Q,et al.Species identification and analyses on intestine bacterial flora of iced fresh large yellow croaker [J].Journal of Chinese Institute of Food Science and Technology,2013,13(12):188-196.
[4] ZHU J L,ZHAO A F,FENG L F,et al.Quorum sensing signals affect spoilage of refrigerated large yellow croaker (Pseudosciaena crocea ) by Shewanella baltica [J].International Journal of Food Microbiology,2016,217:146-155.
[5] LI J P,YU H L,YANG X Y,et al.Complete genome sequence provides insights into the quorum sensing-related spoilage potential of Shewanella baltica 128 isolated from spoiled shrimp[J].Genomics,2020,112(1):736-748.
[6] 梅俊,许振琨.郁慧洁,等.冷链物流中海水鱼的腐败机制及保鲜技术研究进展 [J].食品与生物技术学报,2022,41(7):84-99.MEI J,XU Z K,YU H J,et al.Research progress on spoilage mechanism and preservation technology of marine fish in cold chain logistics [J].Journal of Food Science and Biotechnology,2022,41(7):84-99.
[7] MOSHYNETS O V,FOSTER D,KARAKHIM S A,et al.|Examining c-di-GMP and possible quorum sensing regulation in Pseudomonas fluorescens SBW 25:links between intra-and inter-cellular regulation benefits community cooperative activities such as biofilm formation [J].The Ukrainian Biochemical Journal,2018,90(3):17-31.
[8] 李红艳,徐明磊,翟赛亚.植物提取物在肉制品保鲜中的作用研究进展 [J].食品与机械,2024,40(9):236-240.LI H Y,XU M L,ZHAI S Y.Research progress of plant extracts in preservation of meat products [J].Food & Machinery,2024,40(9):236-240.
[9] 史文艳,孙震.ε-聚赖氨酸盐酸盐与 Nisin 对蜡状芽孢杆菌的协同作用及机理 [J].食品与机械,2019,35(3):15-19,103.SHI W Y,SUN Z.Synergistic effect and mechanism of ε-polylysine hydrochloride and Nisin on Bacillus cereus [J].Food & Machinery,2019,35(3):15-19,103.
[10] 杨华,杨丽丽,王明,等.茶多酚 /ε-聚赖氨酸盐酸盐复合微胶囊 对 美 国 红 鱼 鱼 片 的 保 鲜 作 用 [J].现 代 食 品 科 技,2020,36(1):98-105.YANG H,YANG L L,WANG M,et al.The preservative effect of tea polyphenols/ ε-polylysine hydrochloride composite microcapsules on Sciaennops ocellatus fillets [J]. Modern Food Science and Technology,2020,36(1):98-105.
[11] JIA Z,LI C C,FANG T,et al.Predictive modeling of the effect of ε-polylysine hydrochloride on growth and thermal inactivation of Listeria monocytogenes in fish balls [J].Journal of Food Science,2019,84(1):127-132.
[12] 蓝蔚青,陈雪宁,冯豪杰,等.ε-聚赖氨酸盐酸盐对腐败希瓦氏 菌 的 作 用 机 制 初 探 [J].上 海 海 洋 大 学 学 报,2022,31(6):1 562-1 569.LAN W Q,CHEN X N,FENG H J,et al.Preliminary research on the antibacterial mechanism of ε-polylysine hydrochloride against Shewanella putrefaciens [J].Journal of Shanghai Ocean University,2022,31(6):1 562-1 569.
[13] YANG H,LI Q Y,YANG L L,et al.The competitive release kinetics and synergistic antibacterial characteristics of tea polyphenols/epsilon-poly-L-lysine hydrochloride core-shell microcapsules against Shewanella putrefaciens [J].International Journal of Food Science and Technology,2020,55(12):3 542-3 552.
[14] GE Y,ZHU J,YE X,et al.Spoilage potential characterization of Shewanella and Pseudomonas isolated from spoiled large yellow croaker (Pseudosciaena crocea ) [J].Letters in Applied Microbiology,2017,64(1):86-93.
[15] WIEGAND I,HILPERT K,HANCOCK R E W.Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC ) of antimicrobial substances [J].Nature Protocols,2008,3(2):163-175.
[16] 周倩倩,方士元,梅俊,等.丁香酚对腐败希瓦氏菌和荧光假单胞菌的抑制机理 [J].食品科学,2020,41(9):1-6.ZHOU Q Q,FANG S Y,MEI J,et al.Inhibitory mechanism of eugenol on Shewanella putrefaciens and Pseudomonas fluorescens [J].Food Science,2020,41(9):1-6.
[17] 马子祯,赵前程,李萌,等.山苍子精油与食品添加剂对水产品腐败菌的联合抑制作用研究 [J].食品安全质量检测学报,2020,11(11):3 598-3 606.MA Z Z,ZHAO Q C,LI M,et al.Combined antimicrobial effects of Litsea cubeba essential oil and food additives against spoilage bacteria isolated from aquatic products [J].Journal of Food Safety & Quality,2020,11(11):3 598-3 606.
[18] 王梓源,李欣颖,吕俊阁,等.ε-聚赖氨酸对大肠杆菌的抑菌机制 [J].食品与发酵工业,2020,46(21):34-41.WANG Z Y,LI X Y,LU J G,et al.The antimicrobial mechanism of ε-poly- L-lysine against Escherichia coli [J].Food and Fermentation Industries,2020,46(21):34-41.
[19] 刘海晴,李万鹏,王奕飞,等.酿酒黄水对希瓦氏菌和假单胞菌抑菌机理的研究 [J].食品与发酵工业,2022,48(5):68-74.LIU H Q,LI W P,W Y F,et al.Antibacterial mechanism of yellow water from liquor against Shewanella and Pseudomonas[J].Food and Fermentation Industries,2022,48(5):68-74.
[20] 王晓芸,张婷,黄剑,等.3种多酚对腐败希瓦氏菌的抑菌效果和机理 [J].食品科学,2024,45(16):1-9.WANG X Y,ZHANG T,HUANG J,et al.Antibacterial effects and mechanisms of three polyphenols against Shewanella putrefaciens [J].Food Science,2024,45(16):1-9.
[21] GUO F,LIANG Q,ZHANG M,et al.Antibacterial activity and mechanism of linalool against Shewanella putrefaciens [J].Molecules,2021,26(1):245.
[22] O'TOOLE G,KAPLAN H B,KOLTER R.Biofilm formation as microbial development [J].Annual Review of Microbiology,2000,54:49-79.
[23] 张国丽,敖晓琳,刘书亮.群体感应在食源性微生物生物膜形成中的作用 [J].食品与机械,2017,33(2):194-198.ZHANG G L,AO X L,LIU S L.The role of quorum sensing in biofilm formation of foodborne micro-organism [J].Food & Machinery,2017,33(2):194-198.
[24] WANG Y,WANG Y,CHEN J,et al.Screening and preservation application of quorum sensing inhibitors of Pseudomonas fluorescens and Shewanella baltica in seafood products [J].LWT-Food Science and Technology,2021,149(1):111749.
[25] 赵二科,朱军莉,冯立芳,等.冷藏大黄鱼 SSO 希瓦氏菌致腐能力差异机制初探 [J].水产学报,2015,39(2):256-264.ZHAO E K,ZHU J L,FENG L F,et al.Preliminary mechanism of different spoilage potential of specific spoilage organism,Shewanella,in refrigerated Larimichthys crocea [J].Journal of Fisheries of China,2015,39(2):256-264.
[26] 葛阳杨.大黄鱼源三种优势腐败菌致腐表型及其菌群互作效应研究 [D].杭州:浙江工商大学,2016:46.GE Y Y.Evalluation of spoilage potential and the interactions of three spoilage bacteria isolated from spoiled large yellow croaker [D].Hangzhou:Zhejiang Gongshang University,2016:46.