Preparation technology and antioxidant activity of exopolysaccharide from sulfated Porphyridium aerugineum

Authors

DUAN Dongjie, College of Chemistry and Chemical Engineering , Qinghai Normal University , Xining , Qinghai 810000 , China
ZHANG Wei, College of Chemistry and Chemical Engineering , Qinghai Normal University , Xining , Qinghai 810000 , ChinaFollow
SUI Chengbo, College of Chemistry and Chemical Engineering , Qinghai Normal University , Xining , Qinghai 810000 , China
ZHANG Hao, College of Chemistry and Chemical Engineering , Qinghai Normal University , Xining , Qinghai 810000 , China
ZHANG Guodong, College of Chemistry and Chemical Engineering , Qinghai Normal University , Xining , Qinghai 810000 , China

Corresponding Author(s)

张炜（1972—），女，青海师范大学教授，硕士。E-mail：zhangwei@qhnu.edu.cn

Abstract

［Objective］ To improve the biological activity and application range of Porphyridium aerugineum exopolysaccharide (EPS).［Methods］ EPS was modified using the concentrated sulfuric acid method,with the degree of sulfation as the indicator.The preparation process of sulfated P.aerugineum exopolysaccharide (S-EPS) was optimized using response surface methodology.The physicochemical properties of S -EPS were also determined.［Results］ The optimal conditions were a reaction time of 6 h,a reaction temperature of 26 ℃,and a mass ratio of ammonium sulfate to EPS of 4∶5.Under these conditions,the degree of substitution was 0.807 5±0.008 7.Infrared analysis confirmed that EPS was successfully sulfated.Thermogravimetric analysis showed that the thermal stability of S -EPS was superior to that of EPS.Particle size and Zeta potential analysis indicated that samples with a higher degree of substitution were more stable,with higher —SO3H group content,larger absolute potential,and less aggregation.The results of the antioxidant activity experiment showed that the DPPH·clearance rate of S -EPS was 92.17%,significantly higher than EPS.［Conclusion］ Sulfation modification improved the antioxidant capacity of EPS,indicating that sulfation is a feasible method for enhancing polysaccharides.

Publication Date

4-3-2025

First Page

172

Last Page

179

DOI

10.13652/j.spjx.1003.5788.2024.80581

Recommended Citation

Dongjie, DUAN; Wei, ZHANG; Chengbo, SUI; Hao, ZHANG; and Guodong, ZHANG (2025) "Preparation technology and antioxidant activity of exopolysaccharide from sulfated Porphyridium aerugineum," Food and Machinery: Vol. 41: Iss. 1, Article 22.
DOI: 10.13652/j.spjx.1003.5788.2024.80581
Available at: https://www.ifoodmm.cn/journal/vol41/iss1/22

References

[1] LI S H,JI L,SHI Q W,et al.Advances in the production of bioactive substances from marine unicellular microalgae Porphyridium spp [J].Bioresource Technology,2019,292:122048.
[2] TANNIN-SPITZ T,BERGMAN M,VAN-MOPPES D,et al.Antioxidant activity of the polysaccharide of the red microalga Porphyridium sp[J].Journal of Applied Phycology,2005,17(3):215-222.
[3] LIU L,POHNERT G,WEI D.Extracellular metabolites from industrial microalgae and their biotechnological potential [J].Marine Drugs,2016,14(10):191.
[4] MU S,YANG W J,HUANG G L.Antioxidant activities and mechanisms of polysaccharides [J].Chemical Biology & Drug Design,2021,97(3):628-632.
[5] LIU T B,REN Q Q,WANG S,et al.Chemical modification of polysaccharides:a review of synthetic approaches,biological activity and the structure-activity relationship [J].Molecules,2023,28(16):6 073.
[6] LIANG L,AO L,MA T,et al.Sulfated modification and anticoagulant activity of pumpkin (Cucurbita pepo,Lady Godiva ) polysaccharide [J].International Journal of Biological Macromolecules,2018,106:447-455.
[7] WANG X M,ZHANG Z S,YAO Z Y,et al.Sulfation,anticoagulant and antioxidant activities of polysaccharide from green algae Enteromorpha linza [J].International Journal of Biological Macromolecules,2013,58:225-230.
[8] TAO Y Z,ZHANG R Q,YANG W,et al.Carboxymethylated hyperbranched polysaccharide:synthesis,solution properties,and fabrication of hydrogel [J].Carbohydrate Polymers,2015,128:179-187.
[9] WANG J L,YANG X P,BAO A J,et al.Microwave-assisted synthesis,structure and anti-tumor activity of selenized Artemisia sphaerocephala polysaccharide [J].International Journal of Biological Macromolecules,2017,95:1 108-1 118.|[10] NIU C M,LIU Y N,YANG Y X,et al.Advances in sulfonated modification and bioactivity of polysaccharides [J].International Journal of Biological Macromolecules,2023,253(Pt 6):126400.
[11] DAR A A,ABROL V,SINGH N,et al.Recent bioanalytical methods for the isolation of bioactive natural products from genus Codonopsis [J].Phytochemical Analysis,2023,34(5):491-506.
[12] CHEN Y,YAO F K,MING K,et al.Polysaccharides from traditional Chinese medicines:extraction,purification,modification,and biological activity [J].Molecules,2016,21(12):1 705.
[13] CHEN Y,ZHANG H,WANG Y X,et al.Sulfated modification of the polysaccharides from Ganoderma atrum and their antioxidant and immunomodulating activities [J].Food Chemistry,2015,186:231-238.
[14] LIU W,WANG H Y,YAO W B,et al.Effects of sulfation on the physicochemical and functional properties of a water-insoluble polysaccharide preparation from Ganoderma lucidum[J].Journal of Agricultural and Food Chemistry,2010,58(6):3 336-3 341.
[15] CHEN W W,JIA Z B,ZHU J J,et al.Optimization of ultrasonic-assisted enzymatic extraction of polysaccharides from thick-shell mussel (Mytilus coruscus ) and their antioxidant activities [J].International Journal of Biological Macromolecules,2019,140:1 116-1 125.
[16] 蒋茂婷,冉艳红,刘娜,等.蒜皮水溶性多糖的制备及结构表征[J].食品科学,2022,43(6):57-65.JANG M T,RAN Y H,LIU N,et al.Preparation and structural characterization of water-soluble polysaccharide from garlic skin[J].Food Science,2022,43(6):57-65.
[17] LI X Y,WANG L,WANG B B.Optimization of encapsulation efficiency and average particle size of Hohenbuehelia serotina polysaccharides nanoemulsions using response surface methodology [J].Food Chemistry,2017,229:479-486.
[18] 梁英,毕红梅,郑文凤,等.平菇多糖硫酸酯制备工艺优化及抗氧化活性研究 [J].食品与机械,2021, 37(1):175-179.LIANG Y,BI H M,ZHENG W F,et al.Optimizati on of preparation technology and antioxidant activity of polysaccharide sulfate from Pleurotus ostreatus [J].Food & Machinery,2021,37(1):175-179.
[19] XIE Y T,GUO X,MA Z Y,et al.Efficient extraction and structural characterization of hemicellulose from sugarcane bagasse pith [J].Polymers,2020,12(3):608.
[20] 胡润锋,李浚哲,李鹏飞,等.响应面法优化硒化桑叶多糖的制备工艺及其体外抗氧化活性 [J].中南林业科技大学学报,2022,42(8):148-157.HU R F,LI J Z,LI P F,et al.Optimization of selenized mulberry leaf polysaccharides preparation by response surface methodology and determination of the antioxidant activity in vitro [J].Journal of Central South University of Forestry & Technology,2022,42(8):148-157.
[21] 孙聪聪,庞道睿,黎尔纳,等.响应面法优化 β-半乳糖苷酶法制 备 低 聚 半 乳 糖 工 艺 [J].食 品 工 业 科 技,2022,43(22):246-255.SUN C C,PANG D R,LI E N,et al.Optimization of preparation of galactooligosaccharides by β-galactosidase using response surface methodology [J].Science and Technology of Food Industry,2022,43(22):246-255.
[22] WANG J L,GUO H Y,ZHANG J,et al.Sulfated modification,characterization and structure-antioxidant relationships of Artemisia sphaerocephala polysaccharides [J].Carbohydrate Polymers,2010,81(4):897-905.
[23] ABUDUWAILI A,NUERXIATI R,MUTAILIFU P,et al.Isolation,structural modification,characterization,and bioactivity of polysaccharides from Folium Isatidis [J].Industrial Crops and Products,2022,176:114319.
[24] TANG L Y,SUN Y H,GE P P,et al.Biogenetic nanocarriers with enhanced pH stability formed by zein and selectively depolymerized mushroom hyperbranched β-glucans [J].International Journal of Biological Macromolecules,2022,209:1 771-1 783.
[25] CHEN F,HUANG G L,YANG Z Y,et al.Antioxidant activity of Momordica charantia polysaccharide and its derivatives [J].International Journal of Biological Macromolecules,2019,138:673-680.