Abstract
[Objective] Optimization of Xiaoqu varieties in Guangxi for brewing rice -flavored Baijiu.[Methods] High -throughput sequencing was used to analyze the microbial communities of four Guangxi rice flavored Baijiu Xiaoqu,the physicochemical indexes of Xiaoqu were determined by the conventional methods,then the correlation between physicochemical indexes and the microbial communities was clarified.[Results] Two dominant fungal phyla and nine dominant bacterial phyla were detected in four Xiaoqu samples.The dominant fungal phyla were Ascomycota and Mucoromycota.As the dominant phyla of bacteria,Firmicutes and Proteobacteria are present in all Xiaoqu.Mould was found in all Xiaoqu samples,namely,Rhizopus a was found in Xiaoqu A,B,and C,and Aspergillus and Monascus were found in Xiaoqu D.The common dominant bacterial genera are Pediococcus and Weissella.In terms of physicochemical indexes,the saccharification power,liquefaction power and fermentation power of Xiaoqu A were all the biggest,while the esterification power of Xiaoqu C was greater than that of the other three Xiaoqu.[Conclusion] There is a certain correlation between relevant microorganisms and physicochemical indexes.
Publication Date
4-3-2025
First Page
26
Last Page
34
DOI
10.13652/j.spjx.1003.5788.2024.80403
Recommended Citation
Hui, HOU; Junguang, HAO; Ruijie, WAN; Long, ZHANG; Cen, QI; and Zhiru, DAI
(2025)
"Correlation analysis between microbial community and physicochemical indexes of Xiaoqu in Guangxi,"
Food and Machinery: Vol. 41:
Iss.
1, Article 4.
DOI: 10.13652/j.spjx.1003.5788.2024.80403
Available at:
https://www.ifoodmm.cn/journal/vol41/iss1/4
References
[1] 王毅,罗惠波,王彩虹.小曲酒酿造微生物研究进展 [J].酿酒科技,2014 (4):78-82.WANG Y,LUO H B,WANG C H.Research progress in microbes for the production of Xiaoqu [J].Liquor-Making Science & Technology,2014 (4):78-82.
[2] 边名鸿,万世旅,代梅,等.产酒酵母的筛选鉴定及其在小曲中的应用 [J].中国酿造,2016,35(3):57-60.BIAN M H,WAN S L,DAI M,et al.Screening and identification of Baijiu (liquor )-making yeast and its application in Xiaoqu [J].China Brewing,2016,35(3):57-60.
[3] 黄治国,刘娜,卫春会,等.高温大曲曲房空气中可培养细菌的分离鉴定及产酶特性 [J].食品与机械,2021,37(5):15-21.HUANG Z G,LIU N,WEI C H,et al.Isolation,identification and enzyme production characteristics of culturable bacteria in the air of high-temperature Daqu fermentation room [J].Food & Machinery,2021,37(5):15-21.
[4] 王清龙,朱甜甜,刘延波,等.白酒生产不同酒曲微生物群落结 构、理 化 指 标 及 挥 发 性 风 味 物 质 研 究 [J].中 国 酿 造,2023,42(7):93-102.WANG Q L,ZHU T T,LIU Y B,et al.Microbial community structure,physicochemical indexes and volatile substances of different Jiuqu in Baijiu production [J].China Brewing,2023,42(7):93-102.
[5] WANG Z,SU Z X,YANG Q,et al.Characterizing relationship of microbial community in Xiaoqu and volatiles of light-aroma-type Xiaoqu Baijiu [J].Food Science and Technology Research,2020,26:1-2.
[6] 吴成,程平言,谢丹,等.酱香型白酒高温大曲发酵过程中真菌多样性研究 [J].食品与生物技术学报,2023,42(6):95-103.WU C,CHENG P Y,XIE D,et al.Investigation on fungal diversity during high-temperature Daqu fermentation of sauce-flavor Baijiu [J].Journal of Food Science and Biotechnology,2023,42(6):95-105.
[7] CAPORASO J G,LAUBER C L,WALTERS W A,et al.Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms [J].The ISEM Journal,2012,6(8):1 621-1 624.
[8] GOU M,WANG H Z,YUAN H W,et al.Characterization of the microbial community in three types of fermentation starters used for Chinese liquor production [J].Journal of the Institute of Brewing,2015,121(4):620-627.
[9] WU H C,ZHANG S Y,MA Y Y,et al.Comparison of microbial communities in the fermentation starter used to brew Xiaoqu liquor [J].Journal of the Institute of Brewing,2017,123(1):113-120.
[10] 江威,章刚,张龙,等.添加中药材对绿衣观音土曲品质影响及相关性分析 [J].中国酿造,2024,43(1):169-173.JIANG W,ZHANG G,ZHANG L,et al.Effects of Chinese medicinal materials addition on quality of Green-covering Guanyin Tuqu and correlation analysis [J].China Brewing,2024,43(1):169-173.
[11] 刘小改,王小伟,张坤.清香型白酒大曲酯化力测定方法的优化研究 [J].中国酿造,2021,40(11):184-187.LIU X G,WANG X W,ZHANG K.Optimization of the measurement method for the esterification ability of light-flavor Baijiu Daqu [J].China Brewing,2021,40(11):184-187.
[12] 孙羊羊,尹亚格,吴雨甍,等.基于高通量测序技术分析天津高 温 大 曲 微 生 物 菌 群 多 样 性 [J].中 国 酿 造,2023,42(5):28-34.SUN Y Y,YIN Y G,WU Y M,et al.Analysis of microbial community diversity of Tianjin high-temperature Daqu based on high-throughput sequencing technology [J].China Brewing,2023,42(5):28-34.
[13] WANG J,ZHONG Q P,YANG Y Y,et al.Comparison of bacterial diversity between two traditional starters and the round-koji-maker starter for traditional Cantonese Chi-flavor liquor brewing [J].Frontiers in Microbiology,2018,9:1 053.
[14] 宁亚丽,吴跃,何嫱,等.基于高通量测序技术分析朝鲜族传统 米 酒 及 其 酒 曲 中 微 生 物 群 落 多 样 性 [J].食 品 科 学,2019,40(16):107-114.NING Y L,WU Y,HE Q,et al.Analysis of microbial community diversity in Chinese Korean traditional rice wine and its starter culture using high-throughput sequencing [J].Food Science,2019,40(16):107-114.
[15] ZHAO C,SU W,MU Y,et al.Integrative metagenomics-metabolomics for analyzing the relationship between microorganisms and non-volatile profiles of traditional Xiaoqu[J].Frontiers in Microbiology,2021,11:617030.
[16] 朱治宇.茅台镇酱香型白酒酿造区域霉菌多样性特征研究[D].贵阳:贵州大学,2020:1-68.ZHU Z Y.Study on the characteristics of mold diversity in Maotai town sauce-flavor liquor brewing area [D].Guiyang:Guizhou University,2020:1-68.
[17] 苏凤,卫春会,曾波,等.中高温大曲中红曲霉的筛选及应用[J].食品与机械,2024,40(5):203-209.SU F,WEI C H,ZENG B,et al.Screening and application of Monascus in medium and high temperature Daqu [J].Food & Machinery,2024,40(5):203-209.
[18] 朱丽萍,杨强,江威,等.清香型小曲白酒霉菌菌群解析与酶活特性研究 [J].食品与发酵工业,2022,48(7):70-77.ZHU L P,YANG Q,JIANG W,et al.Mold communities and enzyme activity characteristics in light-flavor Xiaoqu Baijiu[J].Food and Fermentation Industries,2022,48(7):70-77.
[19] 黄科屹,邓杰,卫春会,等.源于中高温大曲的米根霉制备米曲工艺优化及应用 [J].食品与机械,2022,38(9):185-190.HUANG K Y,DE J,WEI C H,et al.Optimization and application of Miqu prepared by Rhizopus oryzae from medium-high temperature Daqu [J].Food & Machinery,2022,38(9):185-190.
[20] ZHAO C,SU W,MU Y C,et al.Effects of Jiuqu inoculating Rhizopus oryzae Q 303 and Saccharomyces cerevisiae on chemical components and microbiota during black glutinous rice wine fermentation [J].International Journal of Food Microbiology,2023,385:110012.
[21] ZHAO C,SU W,MU Y C,et al.Correlations between microbiota with physicochemical properties and volatile flavor components in black glutinous rice wine fermentation [J].Food Research International,2020,138:109800.
[22] HU Y L,LEI X Y,ZHANG X M,et al.Characteristics of the microbial community in the production of Chinese rice-flavor Baijiu and comparisons with the microflora of other flavors of Baijiu [J].Frontiers in Microbiology,2021,12:673670.
[23] WANG D Q,CHEN L Q,YANG F,et al.Yeasts and their importance to the flavour of traditional Chinese liquor:a review [J].Journal of the Institute of Brewing,2019,125(2):214-221.
[24] 郑自强,卫春会,邓杰,等.一株产纤维素酶枯草芽孢杆菌的麸 曲 制 作 及 其 产 酶 特 性 研 究 [J].食 品 与 机 械,2021,37(10):12-17.ZHENG Z Q,WEI C H,DE J,et al.Study on the production of Fuqu and the characteristics of enzyme about a cellulase producing Bacillus subtilis [J].Food & Machinery,2021,37(10):12-17.
[25] DONG W W,YANG Q,LIAO Y X,et al.Characterisation and comparison of the microflora of traditional and pure culture Xiaoqu during the Baijiu liquor brewing process [J].Journal of the Institute of Brewing,2020,126(2):213-220.
[26] 侯强川,王玉荣,田龙新,等.基于宏基因组测序技术解析市售 强 化 酒 曲 微 生 物 群 落 结 构 和 功 能 特 征 [J].食 品 与 发 酵 工业,2023,49(10):100-107.HOU Q C,WANG Y R,TIAN L X,et al.Microbial community structure and functional characteristics of commercial intensified Jiuqu based on metagenomic sequencing technology [J].Food and Fermentation Industries,2023,49(10):100-107.
[27] WANG Q,WANG C Y,XIANG X Q,et al.Analysis of microbial diversity and succession during Xiaoqu Baijiu fermentation using high-throughput sequencing technology [J].Engineering in Life Sciences,2022,22(7):495-504.
[28] 唐佳代,邱树毅,王春晓,等.贵州地区酿酒小曲细菌多样性比较分析 [J].中国酿造,2019,38(10):55-59.TANG J D,QIU S Y,WANG C X,et al.Comparison and analysis of bacterial diversity of brewing Xiaoqu in Guizhou[J].China Brewing,2019,38(10):55-59.
[29] JIANG L,SU W,MU Y C,et al.Major metabolites and microbial community of fermented black glutinous rice wine with different starters [J].Frontiers in Microbiology,2020,11:593.
[30] 冯方剑,唐维川,杨官荣,等.不同产区酱香型大曲理化指标及风味成分的研究 [J].酿酒,2023,50(5):29-36.FENG F J,TANG W C,YANG G R,et al.Research on flavor component and physical index for high temperature Daqu [J].Liquor Making,2023,50(5):29-36.
[31] 王宇良,李志溥,苏泽佳,等.制曲方式对豉香型白酒酒曲理化 因 子 及 细 菌 群 落 的 影 响 [J].食 品 科 学,2023,44(22):211-217.WANG Y L,LI Z P,SU Z J,et al.Effect of preparation methods on the physicochemical properties and bacterial community of Chixiangxing Baijiu Qu [J].Food Science,2023,44(22):211-217.
[32] 皇甫洁,何猛超,刘雅,等.米香型白酒中不同制曲工艺的微生物群落结构差异分析 [J].酿酒科技,2023 (10):17-21.HUANG F J,HE M C,LIU Y,et al.Difference in microbial community structure of Mixiang Xiaoqu by different production technology [J].Liquor-Making Science & Technology,2023 (10):17-21.
[33] 卜光明,周化斌,周茂洪,等.酿造酒中非酿酒酵母的研究进展[J].食品工业科技,2019,40(14):346-352.BU G M,ZHOU H B,ZHOU M H,et al.Research progress on the non-saccharomyces in the brewing wine fermentation [J].Science and Technology of Food Industry,2019,40(14):346-352.
[34] 吴正坤,刘蒲临,杨团元,等.不同贮存期高温大曲微生物群落 演 替 与 理 化 指 标 相 关 性 分 析 [J].中 国 酿 造,2023,42(7):160-166.WU Z K,LIU P L,YANG T Y,et al.Correlation analysis of microbial community succession and physicochemical properties of Daqu in different storage periods [J].China Brewing,2023,42(7):160-166.