Probiotic properties of Pediococcus acidilactici N8 and screening of high-viability protectants

Authors

ZHAO Ying, College of Food Science , South China Agricultural University , Guangzhou , Guangdong 510640 , China ;Guangdong Microecological Formulation Engineering Technology Research Center , Guangzhou , Guangdong 510640 , China
ZOU Yuan, College of Food Science , South China Agricultural University , Guangzhou , Guangdong 510640 , China ;Guangdong Microecological Formulation Engineering Technology Research Center , Guangzhou , Guangdong 510640 , ChinaFollow
YE Zhiwei, College of Food Science , South China Agricultural University , Guangzhou , Guangdong 510640 , China ;Guangdong Microecological Formulation Engineering Technology Research Center , Guangzhou , Guangdong 510640 , China
GUO Liqiong, College of Food Science , South China Agricultural University , Guangzhou , Guangdong 510640 , China ;Guangdong Microecological Formulation Engineering Technology Research Center , Guangzhou , Guangdong 510640 , China
LIN Junfang, College of Food Science , South China Agricultural University , Guangzhou , Guangdong 510640 , China ;Guangdong Microecological Formulation Engineering Technology Research Center , Guangzhou , Guangdong 510640 , China

Corresponding Author(s)

邹苑（1989—），女，华南农业大学食品学院副教授，博士。E-mail：zouyuan@scau.edu.cn

Abstract

[Objective] To investigate the biological characteristics and in vitro probiotic functions of the food-derived Pediococcus acidilactici N8, and to screen effective protectants for the preparation of high-viability (>1012 CFU/g ) spray-dried probiotic preparations.[Methods] A lactic acid bacterium (strain N 8) isolated from cheese was identified through morphological analysis and 16S rDNA gene sequencing.Its probiotic potential was determined through tests on growth characteristics, safety, tolerance, antibacterial activity, adhesion ability, and antioxidant capacity.Spray drying was employed to prepare probiotic formulations, and high-viability spray-drying protectants were screened. [Results] Based on morphological characteristics and 16S rDNA gene sequence analysis, strain N 8 was identified as P.acidilactici.It exhibited a rapid S-shaped growth curve and probiotic traits including non-hemolytic activity and sensitivity to multiple antibiotics.Its supernatant effectively inhibited the growth of various pathogenic bacteria.The strain showed strong stability under heat, varying pH levels, bile salts, and simulated gastrointestinal fluid conditions, and possessed strong adhesion ability.Both the cell-free supernatant and intact cell suspension of P.acidilactici N8 demonstrated strong scavenging abilities against DPPH, ABTS, and hydroxyl radicals.When buffalo milk, Cordyceps militaris polysaccharides, and sodium glutamate were used as protectants, the viable count of the probiotic preparation reached 1012 CFU/g. [Conclusion] P.acidilactici N8 is a food-derived lactic acid bacterium with good probiotic potential.Using buffalo milk, C.militaris polysaccharides, and sodium glutamate as spray-drying protectants can yield high-viability 林俊芳 (1962 —), 男, 华南农业大学食品学院研究员, 博士。E-mail：linjf@scau.edu.cn probiotic preparations.

Publication Date

11-19-2025

First Page

1

Last Page

10

DOI

10.13652/j.spjx.1003.5788.2025.80007

Recommended Citation

Ying, ZHAO; Yuan, ZOU; Zhiwei, YE; Liqiong, GUO; and Junfang, LIN (2025) "Probiotic properties of Pediococcus acidilactici N8 and screening of high-viability protectants," Food and Machinery: Vol. 41: Iss. 10, Article 1.
DOI: 10.13652/j.spjx.1003.5788.2025.80007
Available at: https://www.ifoodmm.cn/journal/vol41/iss10/1

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