Abstract
[Objective] To study the effect of the enzymatic hydrolysis process on the flavor compounds of Lentinula edodes. [Methods] The optimized enzymatic hydrolysis process is adopted, with free amino acid, 5'-nucleotide, equivalent umami concentration (EUC ), volatile flavor compounds, and sensory evaluation as indicators, to explore the effect of enzymatic hydrolysis process on flavor compounds of Lentinula edodes from multiple perspectives. [Results] After enzymatic hydrolysis, the free amino acid content in Lentinula edodes is 19. 85 mg/g, 1. 45 times higher than that before enzymatic hydrolysis, with glutamic acid and aspartic acid increasing by 83. 80% and 27. 13%, respectively. The 5'-nucleotide is 0.44 mg/g, 1. 76 times higher than that before enzymatic hydrolysis, with 5'-GMP increasing by 166. 67% and 5-AMP by 125. 00%. The EUC is 63. 35 g/100 g, 2. 03 times higher than that before enzymatic hydrolysis. There are also significant differences in the types and quantities of volatile components before and after enzymatic hydrolysis. The sensory score is significantly improved after enzymatic hydrolysis. [Conclusion] The enzymatic hydrolysis process significantly enhances the non-volatile flavor compounds of Lentinula edodes and noticeably changes their volatile components.
Publication Date
11-19-2025
First Page
186
Last Page
193
DOI
10.13652/j.spjx.1003.5788.2024.81170
Recommended Citation
Guomei, CUI; Shunfeng, LI; Shuaiping, GAO; Lina, LIU; Yating, HU; Fangfang, XU; and Anjian, WANG
(2025)
"Effect of enzymatic hydrolysis process on flavor compounds of Lentinula edodes,"
Food and Machinery: Vol. 41:
Iss.
10, Article 24.
DOI: 10.13652/j.spjx.1003.5788.2024.81170
Available at:
https://www.ifoodmm.cn/journal/vol41/iss10/24
References
[1] 谷 镇,杨 焱.食 用 菌 呈 香 呈 味 物 质 研 究 进 展 [J].食 品 工 业 科技,2013,34(5):363-367.GU Z,YANG Y.Research progress in flavor components of edible fungus [J].Science and Technology of Food Industry,2013,34(5):363-367.
[2] KOMAT A.The taste and constituents of foods [J].Nippon Sho-kuhin Kogyo Gakkaishi,1969,3:26.
[3] 张婷婷,丁婼微,张宾乐.香菇酶法制备调味品基料的工艺优化[J].食品工业科技,2021,42(8):199-205.ZHANG T T,DING N W,ZHANG B L.Process optimization of enzymatic preparation of condiment base material by Lentinus edodes [J].Science and Technology of Food Industry,2021,42(8):199-205.
[4] DU J X,XI J P,CHEN X,et al.Effects of different extraction methods on the release of non-volatile flavor components in shiitake mushroom (Lentinus edodes ) [J].Journal of Food Composition and Analysis,2024,128:106001.
[5] 李顺峰,刘丽娜,王安建,等.香菇煮菇水木瓜蛋白酶酶解增鲜技术研究 [J].食品与机械,2020,36(6):188-192.LI S F,LIU L N,WANG A J,et al.Enzymatic hydrolysis to enhance the umami taste of Lentinus edodes boiling water by papain [J].Food & Machinery,2020,36(6):188-192.
[6] 刘丽娜,李顺峰,魏书信,等.不同蛋白酶对香菇酶解液性质的影响 [J].中国酿造,2022,41(3):152-157.LIU L N,LI S F,WEI S X,et al.Effects of different protease on the property of Lentinus edodes enzymatic hydrolysate [J].China Brewing,2022,41(3):152-157.
[7] 李顺峰,刘丽娜,王安建,等.香菇煮菇水 5'-磷酸二酯酶酶解增鲜技术 [J].河南农业科学,2021,50(2):167-172.LI S F,LIU L N,WANG A J,et al.Enzymatic hydrolysis to enhance umami taste of Lentinus edodes boiling water by 5'-phosphodiesterase [J].Journal of Henan Agricultural Sciences,2021,50(2):167-172.
[8] 陈钧辉,陶力,李俊,等.生物化学实验 [M].3版.北京:科学出版社,2003 .CHEN J H,TAO L,LI J,et al.Biochemistry protocols [M].3rd ed.Beijing:Science Press,2003.
[9] 陈万超,杨焱,冯杰,等.不同产地商业品种香菇的滋味成分分析及评价 [J].食品工业科技,2015,36(8):152-157,166.CHEN W C,YANG Y,FENG J,et al.Analysis and evaluation of taste components in dried Lentinula edodes collected from different areas [J].Science and Technology of Food Industry,2015,36(8):152-157,166.
[10] 刘芹,崔筱,孔维丽,等.不同成熟度香菇鲜味物质的比较分析[J].食品与生物技术学报,2023,42(4):94-102.LIU Q,CUI X,KONG W L,et al.Comparative analysis of taste component in shiitake mushroom (Lentinula edodes ) harvested at different stages of maturity [J].Journal of Food Science and Biotechnology,2023,42(4):94-102.
[11] YAMAGUCHI S,YOSHIKAWA T,IKEDA S,et al.Measurement of the relative taste intensity of some L-α-amino acids and 5'-nucleotides [J].Journal of Food Science,1971,36(6):846-849.
[12] 姜宁,董浩然,李巧珍,等.不同光质培养下香菇挥发性风味物质的比较分析 [J].上海农业学报,2023,39(6):32-40.JIANG N,DONG H R,LI Q Z,et al.Comparative analysis of volatile components of Lentinus edodes cultured with different light illumination [J].Acta Agriculturae Shanghai,2023,39(6):32-40.
[13] 律诗,代晹鑫,刘野,等.食用菌鲜味强度评价及鲜味氨基酸和核苷酸提取工艺优化 [J].食品科学技术学报,2022,40(1):100-108.LÜ S,DAI Y X,LIU Y,et al.Evaluation of umami intensity of edible fungi and optimization of umami amino acid andnucleotide extraction [J].Journal of Food Science and Technology,2022,40(1):100-108.
[14] AISALA H,MANNINEN H,LAAKSONEN T,et al.Linking volatile and non-volatile compounds to sensory profiles and consumer liking of wild edible Nordic mushrooms [J].Food Chemistry,2020,304:125403.
[15] LIU Q,BAU T,JIN R X,et al.Comparison of different drying techniques for shiitake mushroom (Lentinus edodes ):changes in volatile compounds,taste properties,and texture qualities[J].LWT-Food Science Technology,2022,164:113651.
[16] 于慧萍,胡思,黄文,等.干制方式对大球盖菇滋味物质的影响[J].食品工业科技,2021,42(9):251-256.YU H P,HU S,HUANG W,et al.Effects of drying process on the tasty components in Stropharia rugoso-annulata [J].Science and Technology of Food Industry,2021,42(9):251-256.
[17] HOU H,LIU C,LU X S,et al.Characterization of flavor frame in shiitake mushrooms (Lentinula edodes ) detected by HS-GC-IMS coupled with electronic tongue and sensory analysis:influence of drying techniques [J].LWT-Food Science and Technology,2021,146:111402.
[18] YANG J H,LIN H C,MAU J L.Non-volatile taste components of several commercial mushrooms [J].Food Chemistry,2001,72(4):465-471.
[19] 李晓龙,杨焱,李文,等.不同烹饪方式对鹿茸菇营养特性和非 挥 发 性 风 味 物 质 的 影 响 [J].食 品 工 业 科 技,2025,46(3):304-312.LI X L,YANG Y,LI W,et al.Effects of different cooking methods on nutritional properties and non-volatile flavor substances of Lyophyllum decastes [J].Science and Technology of Food Industry,2025,46(3):304-312.
[20] 李延年.香菇复配草菇酶解呈味物质的研究与产品开发 [D].泰安:山东农业大学,2021:40-41.LI Y N.Study on flavor substances of the enzymatic hydrolysate of Lentinus edodes mixed with Volvaria volavacea and product development [D].Taian:Shandong Agricultural University,2021:40-41.
[21] LIU Y,HUANG F,YANG H,et al.Effects of preservation methods on amino acids and 5'-nucleotides of Agaricus bisporus mushrooms [J].Food Chemistry,2014,149:221-225.
[22] WANG L Q,HU Q H,PEI F,et al.Influence of different storage conditions on physical and sensory properties of freeze-dried Agaricus bisporus slices [J].LWT-Food Science and Technology,2018,97:164-171.
[23] RANGEL-VARGAS E,RODRIGUEZ J A,DOMÍNGUEZ R,et al.Edible mushrooms as a natural source of food ingredient/additive replacer [J].Foods,2021,10(11):2 687.
[24] MAU J L.The umami taste of edible and medicinal mushrooms [J].International Journal of Medicinal Mushrooms,2005,7(1/2):119-126.
[25] 杜佳馨,席嘉佩,方东路,等.猪肚菌不同部位的营养评价及风味特征分析 [J].浙江大学学报 (农业与生命科学版 ),2021,47(6):743-756.DU J X,XI J P,FANG D L,et al.Nutritional evaluation and flavor characteristic analysis of different parts of Clitocybe maxima [J].Journal of Zhejiang University (Agriculture and Life Sciences ),2021,47(6):743-756.
[26] 陈俊宇,凌建刚,杜丽慧,等.基于 GC-IMS 技术分析不同干燥 方 式 花 菇 的 挥 发 性 风 味 成 分 差 异 [J].食 品 与 机 械,2024,40(6):43-52.CHEN J Y,LING J G,DU L H,et al.Differents in volatile components between different drying methods of Lentinula edodes analyzed by GC-IMS [J].Food & Machinery,2024,40(6):43-52.
[27] 王海波,钟丹,林芷欣,等.4种番石榴绿熟期和黄熟期的挥发性物质差异比较 [J].食品与机械,2024,40(6):176-184,191.WANG H B,ZHONG D,LIN Z X,et al.Comparison of volatile compounds between the green and yellow ripening fruits of four guava species [J].Food & Machinery,2024,40(6):176-184,191.
[28] SONG Y T,DU B F,DING Z H,et al.Baked red pepper (Capsicum annuum L.) powder flavor analysis and evaluation under different exogenous Maillard reaction treatment [J].LWT-Food Science and Technology,2021,139:110525.
[29] CHEN L P,TENG X Y,LIU Y,et al.The dynamic change of flavor characteristics in Pacific oyster (Crassostrea gigas ) during depuration uncovered by mass spectrometry-based metabolomics combined with gas chromatography-ion mobility spectrometry (GC-IMS ) [J].Food Chemistry,2024,434:137277.
[30] 贾效函,沈才洪,傅其旭,等.气相色谱—离子迁移谱法分析烹 调 白 酒 对 鸡 胗 去 腥 增 香 的 效 果 [J].食 品 与 机 械,2025,41(1):208-218.JJIA X H,SHEN C H,FU Q X,et al.Deodorizing and aroma-enhancing effects of different cooking Baijiu products on chicken gizzards based on gas chromatography-ion mobility spectrometry [J].Food & Machinery,2025,41(1):208-218.
[31] 吴思雨,谢萱,刘雨雯,等.基于电子鼻、HS-SPME-GC-MS 和HS-GC-IMS 评价不同制油工艺对大豆油品质及风味的影响[J].食品科学,2024,45(4):183-196.WU S Y,XIE X,LIU Y W,et al.Effects of different production processes on the quality and flavor of soybean oil as evaluated by electronic nose,HS-SPME-GC-MS and HS-GC-IMS [J].Food Science,2024,45(4):183-196.