Abstract
[Objective] To develop a colorimetric analysis method for the rapid detection of H2O2 in milk. [Methods] A novel composite nanozyme material, Ag/g-C3N4, is synthesized by loading nano-Ag on the surface of g-C3N4 nanosheets via a reduction method. The structure and morphology of the material are characterized by X-ray diffraction and transmission electron microscopy. The catalytic conditions for the TMB-H2O2 colorimetric reaction are optimized, and catalytic mechanisms and steady-state kinetics are analyzed. [Results] The Ag/g-C3N4 material exhibits good peroxidase-like activity, and the enzyme-catalyzed reaction follows the Michaelis-Menten kinetic model. The optimal detection range for H2O2 in the colorimetric system is 10~50 μmol/L, with a detection limit of 0.05 μmol/L. When applied to detect H2O2 in real milk samples, the recovery rate is between 99. 47% and 102. 83%. [Conclusion] The developed colorimetric analysis method is simple to prepare, cost-effective, highly sensitive, and demonstrates good operability and practicality.
Publication Date
11-19-2025
First Page
27
Last Page
33
DOI
10.13652/j.spjx.1003.5788.2024.81316
Recommended Citation
Yanfeng, ZHU and Songnan, WANG
(2025)
"Detection for hydrogen peroxide in milk by the nanozyme based on Ag/g-C 3N4,"
Food and Machinery: Vol. 41:
Iss.
10, Article 4.
DOI: 10.13652/j.spjx.1003.5788.2024.81316
Available at:
https://www.ifoodmm.cn/journal/vol41/iss10/4
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