Abstract
[Objective] To develop an ultra -high performance liquid chromatography -tandem mass spectrometry (UPLC -MS/MS ) method for determining lutein and natural vitamin K (VK) homologues, including phylloquinone (VK1), menaquinone -4 (MK-4), menaquinone -7 (MK-7), and menaquinone -9 (MK-9), in poultry eggs by optimizing ultrasound assisted enzymatic hydrolysis. [Methods] The target compound is extracted from poultry egg samples by ultrasound assisted enzymatic hydrolysis and then separated on C18 (100 mm ×2.1 mm, 3.0 μm) column with 0.1% formic acid aqueous solution -0.1% formic acid methanol for gradient elution.Meanwhile, the samples are quantified by external standard with APCI source for detection and analysis. [Results] Under the ultrasound assisted enzymatic hydrolysis conditions of enzyme dosage of 1 500 U and hydrolysis time of 1 hour, the linear relationships of lutein and natural VK homologues are good in the range from 1.0 ng/mL to 5 000 ng/mL (R2>0.996).The detection limit is 0.8~1.5 μg/100 g, and the quantification limit is 2.0~5.0 μg/100 g.The average recovery of lutein and natural VK homologues is 80.1%~94.9% when the spiked concentration is 10, 20, and 100 μg/100 g with the RSD of 1.3%~5.5%. [Conclusion] This method has high detection efficiency, sensitivity, and selectivity and can be used as a detection method for lutein and natural VK homologues in poultry eggs.
Publication Date
12-11-2025
First Page
35
Last Page
40
DOI
10.13652/j.spjx.1003.5788.2025.80124
Recommended Citation
Zhenlin, CAI and Tao, LI
(2025)
"Ultrasound assisted enzymatic hydrolysis combined with ultra-high performance liquid chromatography-tandem mass spectrometry for determination of lutein and natural vitamin K homologues in poultry eggs,"
Food and Machinery: Vol. 41:
Iss.
11, Article 5.
DOI: 10.13652/j.spjx.1003.5788.2025.80124
Available at:
https://www.ifoodmm.cn/journal/vol41/iss11/5
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