Abstract
Camellia nitidissima Chi polysaccharide is a natural polymer compound with multiple biological activities,including significant antioxidant,antibacterial,and hypoglycemic effects,which has attracted considerable attention.However,research on C.nitidissima Chi polysaccharides still faces issues such as a limited range of extraction methods,insufficient studies on separation and purification,and structural characterization focused mainly on primary structure.This paper provides a systematic review of the current research on C.nitidissima Chi polysaccharides,covering extraction processes,separation and purification,structural characterization,and biological properties,while also discussing future research directions.
Publication Date
4-25-2025
First Page
228
Last Page
235
DOI
10.13652/j.spjx.1003.5788.2024.80691
Recommended Citation
Furong, ZHU; Zhong, CHENG; Yingtong, TANG; Haiyan, XU; and Siqi, LIANG
(2025)
"Research progress on the extraction , purification , structure , and bioactivity of polysaccharides from Camellia nitidissima Chi,"
Food and Machinery: Vol. 41:
Iss.
3, Article 31.
DOI: 10.13652/j.spjx.1003.5788.2024.80691
Available at:
https://www.ifoodmm.cn/journal/vol41/iss3/31
References
[1] ZHANG H L,WU Q X,WEI X,et al.Pancreatic lipase and cholesterol esterase inhibitory effect of Camellia nitidissima Chi flower extracts in vitro and in vivo [J].Food Bioscience,2020,37:100682.
[2] ZHANG X M,FENG J,SU S F,et al.Hepatoprotective effects of Camellia nitidissima aqueous ethanol extract against CCl4-induced acute liver injury in SD rats related to Nrf 2 and NF- κB signalling [J].Pharmaceutical Biology,2020,58(1):239-246.
[3] HE D Y,LI X Y,SAI X,et al.Camellia nitidissima C.W.Chi:a review of botany,chemistry,and pharmacology [J].Phytochemistry Reviews,2018,17(2):327-349.
[4] QI J,SHI R F,YU J M,et al.Chemical constituents from leaves of Camellia nitidissima and their potential cytotoxicity on SGC 7901 cells [J].Chinese Herbal Medicines,2016,8(1):80-84.
[5] WANG Y X,ZHANG T,HUANG X J,et al.Heteroglycans from the fruiting bodies of agrocybe cylindracea:fractionation,physicochemical properties and structural characterization [J].Food Hydrocolloids,2021,114:106568.
[6] 牛广俊,朱思,陈清英,等.金花茶不同部位多糖的测定及体外抗氧化活性 [J].中国实验方剂学杂志,2014,20(20):168-172.NIU G J,ZHU S,CHEN Q Y,et al.Determination polysaccharides and antioxidant activity of different parts of camellia chrysantha [J].Chinese Journal of Experimental Traditional Medical Formulae,2014,20(20):168-172.
[7] XU Y Q,JI W B,YU P G,et al.Effect of extraction methods on the chemical components and taste quality of green tea extract[J].Food Chemistry,2018,248:146-154.
[8] LEI P Y,CHEN H J,MA J H,et al.Research progress on extraction technology and biomedical function of natural sugar substitutes [J].Frontiers in Nutrition,2022,9:952147.
[9] DREVELEGKA I,GOULA A M.Recovery of grape pomace phenolic compounds through optimized extraction and adsorption processes [J].Chemical Engineering and Processing-Process Intensification,2020,149:107845.
[10] CHENG Y,XUE F M,YANG Y.Hot water extraction of antioxidants from tea leaves-optimization of brewing conditions for preparing antioxidant-rich tea drinks [J].Molecules,2023,28(7):3 030.
[11] 龚雯,唐婕,韦雅渊,等.金花茶多糖分离纯化、结构表征及其体外抗氧化性 [J].食品与机械,2021,37(6):184-190.GONG W,TANG J,WEI Y Y,et al.Isolation,purification,structural characterization of polysaccharide from Camellia nitidissima Chi and its antioxidant activities in vitro [J].Food & Machinery,2021,37(6):184-190.
[12] CHEN X H,CHEN G J,WANG Z R,et al.A comparison of a polysaccharide extracted from ginger (Zingiber officinale ) stems and leaves using different methods:preparation,structure characteristics,and biological activities [J].International Journal of Biological Macromolecules,2020,151:635-649.
[13] QURESHI F,YUSUF M,KHAN M A,et al.A state-of-the-art review on the latest trends in hydrogen production,storage,and transportation techniques [J].Fuel,2023,340:127574.
[14] YAO J M,LIU H F,MA C Y,et al.A review on the extraction,bioactivity,and application of tea polysaccharides [J].Molecules,2022,27(15):4 679.
[15] GHARIBZAHEDI S M T,MARTI-QUIJAL F J,BARBA F J,et al.Current emerging trends in antitumor activities of polysaccharides extracted by microwave-and ultrasound-assisted methods [J].International Journal of Biological Macromolecules,2022,202:494-507.
[16] MANDAL C,JAISWAL M,BOSCO S J D,et al.Process optimization for ultrasound and microwave-assisted phytonutrients extractions from Averrhoa carambola fruit [J].Biomass Conversion and Biorefinery,2024,14(15):18 253-18 265.
[17] 刘茜,许子竞,胡旭飞.响应面微波辅助提取金花茶花多糖工艺研究 [J].湖南师范大学自然科学学报,2016,39(3):40-45.LlU Q,XU Z J,HU X F.Study on the microwave-assisted extraction of polysaccharides from Camellia chrysantha (Hu) |Tuyama flowers by response surface analysis [J].Journal of Natural Science of Hunan Normal University,2016,39(3):40-45.
[18] OYEYINKA S A,AKINTAYO O A,ADEBO O A,et al.A review on the physicochemical properties of starches modified by microwave alone and in combination with other methods[J].International Journal of Biological Macromolecules,2021,176:87-95.
[19] CHEN X X,YANG J,SHEN M Y,et al.Structure,function and advance application of microwave-treated polysaccharide:a review [J].Trends in Food Science & Technology,2022,123:198-209.
[20] CHEN Y Y,GU X H,HUANG S Q,et al.Optimization of ultrasonic/microwave assisted extraction (UMAE ) of polysaccharides from Inonotus obliquus and evaluation of its anti-tumor activities [J].International Journal of Biological Macromolecules,2010,46(4):429-435.
[21] 韦璐,秦小明,黄日秋,等.超声波提取金花茶多糖的工艺研究[J].食品科技,2007,32(11):100-102.WEI L,QIN X M,HUANG R Q,et al.Study on tea polysaccharide from the leave of Camellia chrysantha (Hu) Tuyama by the ultrasonic extraction process [J].Food Science and Technology,2007,32 (11):100-102.
[22] 许子竞,廖敏富,陈海燕,等.金花茶叶多糖超声波辅助提取工艺优化和含量测定 [J].食品科学,2010,31(4):53-58.XU Z J,LIAO M F,CHEN H Y,et al.Camellia chrysantha (Hu) Tuyama leaves:optimization of ultrasonic-assisted extraction and methodological evaluation of phenol-sulfuric acid determination of polysaccharide [J].Food Science,2010,31(4):53-58.
[23] WU J,CHEN R Z,TAN L,et al.Ultrasonic disruption effects on the extraction efficiency,characterization,and bioactivities of polysaccharides from Panax notoginseng flower [J].Carbohydrate Polymers,2022,291:119535.
[24] WANG H S,CHEN J R,REN P F,et al.Ultrasound irradiation alters the spatial structure and improves the antioxidant activity of the yellow tea polysaccharide [J].Ultrasonics Sonochemistry,2021,70:105355.
[25] BAIK J H,SHIN K S,PARK Y,et al.Biotransformation of catechin and extraction of active polysaccharide from green tea leaves via simultaneous treatment with tannase and pectinase[J].Journal of the Science of Food and Agriculture,2015,95(11):2 337-2 344.
[26] 王元凤,魏新林,王登良,等.金花茶叶酶法提取工艺条件的研究 [J].无锡轻工大学学报,2001,20(6):582-587.WANG Y F,WEI X L,WANG D L,et al.Studying on enzymatic extraction process condition of yellow flower camellia [J].Journal of Food Science and Biotechnology,2001,20(6):582-587.
[27] 王若男,厉荣玉,郑鹏,等.黄精多糖微生物发酵提取、表征及其抗氧化活性分析 [J].中国食品添加剂,2022,33(9):54-62.WANG R N,LI R Y,ZHENG P,et al.Microbial fermentation extraction,characterization and antioxidant activity of Polygonatum polysaccharide [J].China Food Additives,2022,33(9):54-62.
[28] 田晓春,秦小明,林华娟,等.金花茶多糖理化性质的研究[J].中国食品学报,2011,11(8):47-52.TIAN X C,QIN X M,LIN H J,et al.Studies on physicochemical characteristic of polysaccharides from leaves of Camellia chrysantha (Hu) Tuyama [J].Journal of Chinese Institute of Food Science and Technology,2011,11(8):47-52.
[29] ZENG X T,LI P Y,CHEN X,et al.Effects of deproteinization methods on primary structure and antioxidant activity of Ganoderma lucidum polysaccharides [J].International Journal of Biological Macromolecules,2019,126:867-876.
[30] 方苏,闫兴富,周立彪.脱脂和脱蛋白处理对虫草多糖测定的影响 [J].江苏农业科学,2017,45(9):144-146.FANG S,YAN X F,ZHOU L B.Effect of degreasing and deproteinized treatments on Cordyceps polysaccharide assays [J].Jiangsu Agricultural Science,2017,45(9):144-146.
[31] CHEN Y,XIE M Y,LI W J,et al.An effective method for deproteinization of bioactive polysaccharides extracted from lingzhi (Ganoderma atrum ) [J].Food Science and Biotechnology,2012,21(1):191-198.
[32] HUANG G L,SHU S Q,CAI T T,et al.Preparation and deproteinization of garlic polysaccharide [J].International Journal of Food Sciences and Nutrition,2012,63(6):739-741.
[33] FIC E,KEDRACKA-KROK S,JANKOWSKA U,et al.Comparison of protein precipitation methods for various rat brain structures prior to proteomic analysis [J].Electrophoresis,2010,31(21):3 573-3 579.
[34] QIN C G,HUANG K X,XU H B.Isolation and characterization of a novel polysaccharide from the mucus of the loach,Misgurnus anguillicaudatus [J].Carbohydrate Polymers,2002,49(3):367-371.
[35] LI X,ZHAO R,ZHOU H L,et al.Deproteinization of polysaccharide from the stigma maydis by sevag method [J].Advanced Materials Research,2011,340:416-420.
[36] 蔡静.飞天蜈蚣七多糖提取纯化工艺、结构及抗氧化性研究[D].西安:西北大学,2011:23-32.CAI J.Study on extraction and purification process,structure and antioxidant activity of polysaccharides from Pteris icropo[J].Xi'an:Northwestern University,2011:23-32.
[37] 李知敏,王伯初,周菁,等.植物多糖提取液的几种脱蛋白方法的比较分析 [J].重庆大学学报 (自然科学版 ),2004,27(8):57-59.LI Z M,WANG B C,ZHOU J,et al.Comparison of three methods of removing protein from polysaccharide extract in the plant [J].Journal of Chongqing University (Natural Science Edition ),2004,27(8):57-59.
[38] ZHANG S J,HU T T,CHEN Y Y,et al.Analysis of the polysaccharide fractions isolated from pea (Pisum sativum L.) at different levels of purification [J].Journal of Food Biochemistry,2020,44(8):e13248.
[39] 覃梦琳.毛细管电泳测定金花茶,六堡茶中多糖组成和食品添加剂的研究 [D].南宁:广西大学,2016:16-25.QIN M L.Detemination of polysaccharide composition of Camellia nitidissima Chi,Liupao tea and food additives by capillary electrophresis [D].Nanning:Guangxi University,2016:16-25.
[40] HU Z Y,ZHOU H L,LI Y P,et al.Optimized purification process of polysaccharides from Carex meyeriana Kunth by macroporous resin,its characterization and immunomodulatory activity [J].International Journal of Biological Macromolecules,2019,132:76-86.
[41] HU Z Y,YU R Y,SUN J F,et al.Static decolorization of polysaccharides from the leaves of Rhododendron dauricum:process optimization,characterization and antioxidant activities[J].Process Biochemistry,2022,121:113-125.
[42] WANG P Y,ZHAO B L,YIN Z T,et al.Structure elucidation and anticancer activity of a heteropolysaccharide from white tea[J].Carbohydrate Polymers,2024,333:121976.
[43] SHAO L J,SUN Y,LIANG J,et al.Decolorization affects the structural characteristics and antioxidant activity of polysaccharides from Thesium chinense Turcz:comparison of activated carbon and hydrogen peroxide decolorization [J].International Journal of Biological Macromolecules,2020,155:1 084-1 091.
[44] BELWAL T,LI L,XU Y Q,et al.Ultrasonic-assisted modifications of macroporous resin to improve anthocyanin purification from a Pyrus communis var.Starkrimson extract[J].Ultrasonics Sonochemistry,2020,62:104853.
[45] ZHOU R,WANG Y T,LI C,et al.A preliminary study on preparation,characterization,and prebiotic activity of a polysaccharide from the edible mushroom Ramaria flava [J].Journal of Food Biochemistry,2022,46(9):e14371.
[46] SHI S,WANG G C,LIU J,et al.Gentiana straminea Maxim.polysaccharide decolored via high-throughput graphene-based column and its anti-inflammatory activity [J].International Journal of Biological Macromolecules,2021,193:1 727-1 733.
[47] SHI S,ZHANG W T,LIU X N,et al.A sustainable and nondestructive method to high-throughput decolor Lycium barbarum L.polysaccharides by graphene-based nano-decoloration [J].Food Chemistry,2021,338:127749.
[48] 路垚,林华娟,杨基住.凝集素亲和层析技术分离金花茶多糖的研究 [J].农业研究与应用,2014 (4):1-8,16.LU Y,LIN H J,YANG J Z.Study on the lectin affinity chromatography of polysaccharides in Camellia chrysantha (Hu) Tuyama [J].Agricultural Research and Application,2014(4):1-8,16.
[49] 林华娟,田晓春,秦小明,等.金花茶多糖单一成分的化学结构特征解析 [J].食品科学,2013,34(3):141-146.LIN H J,TIAN X C,QIN X M,et al.Structural characteristics of purified polysaccharide from Camellia chrysantha (Hu) Tuyams [J].Food Science,2013,34(3):141-146.
[50] FERREIRA S S,PASSOS C P,MADUREIRA P,et al.Structure-function relationships of immunostimulatory polysaccharides:a review [J].Carbohydrate Polymers,2015,132:378-396.
[51] NIE S P,XIE M Y.A review on the isolation and structure of tea polysaccharides and their bioactivities [J].Food Hydrocolloids,2011,25(2):144-149.
[52] 刘永玲,赵建国,赵治兵,等.PMP柱前衍生 HPLC法测定八月瓜果皮多糖中单糖组成 [J].食品研究与开发,2023,44(21):131-137.LIU Y L,ZHAO J G,ZHAO Z B,et al.Determination of monosaccharide composition of polysaccharides from akebia trifoliate peel by PMP-HPLC with pre-column derivatization[J].Food Research and Development,2023,44(21):131-137.
[53] FENG Z H,DENG L M,CHEN X L,et al.Structural characterization and anti-pigmentation of a novel heteropolysaccharide from Gracilaria lemaneiformis via α-MSH/MC 1R pathway [J].Journal of Functional Foods,2023,107:105650.
[54] CAMPO-GRANDE G C,DA LUZ B B,MARIA-FERREIRA D,et al.Water-soluble polysaccharides from Piper regnellii (Pariparoba ) leaves:structural characterization and antinociceptive and anti-inflammatory activities [J].Carbohydrate Polymers,2023,319:121142.
[55] MIAO X L,MA H M,KE Q H,et al.The determination of monosaccharide in different years Qingzhuan dark tea polysaccharide by liquid chromatography-mass spectrometry[J].Phytochemical Analysis,2022,33(4):577-589.
[56] 张玉梅,邢慧珍,刘会平,等.槐花多糖的提取、纯化和抗氧化活性分析 [J].食品工业科技,2023,44(24):207-215.ZHANG Y M,XING H Z,LIU H P,et al.Extraction,purification and antioxidant activity of polysaccharides from Sophora japonica [J].Science and Technology of Food Industry,2023,44(24):207-215.
[57] 蒋文明,周石洋.海棠果多糖提取、结构表征以及生物活性研究 [J].中国食品添加剂,2023,34(7):81-89.JIANG W M,ZHOU S Y.Extraction,structure characterization and biological activity of polysaccharides from Malus prunifolia [J].China Food Additives,2023,34(7):81-89.
[58] 刘青,李月,杨润梅,等.金花茶组植物资源现状与现代研究进展 [J].中国现代中药,2021,23(4):727-733.LIU Q,LI Y,YANG R M.Yellow camellia:resource status and research progress in modern studies [J].Modern Chinese Medicine,2021,23(4):727-733.|[59] 韦璐,秦小明,林华娟,等.金花茶多糖的降血脂功能研究[J].食品科技,2008 (7):247-249.WEI L,QIN X M,LIN H J,et al.Study on the hypolipidemia activity of polysaccharides from the leaves of Camellia chrysantha (Hu) Tuyama [J].Food Science and Technology,2008 (7):247-249.
[60] 陆建平,覃梦琳,布静龙,等.胶束电动毛细管色谱法测定金花茶和六堡茶中多糖的单糖组成 [J].理化检验 (化学分册 ),2017,53(4):442-446.LU J P,QIN M L,BU J L,et al.MECC determination of monosaccharide composition of polysaccharides in camellia and Liupu teas [J].Physical Testing and Chemical Analysis (Part B:Chemical Analysis ),2017,53(4):442-446.
[61] ZHENG Z M,PAN X L,LUO L,et al.Advances in oral absorption of polysaccharides:mechanism,affecting factors,and improvement strategies [J].Carbohydrate Polymers,2022,282:119110.
[62] FORSBERG Z,STEPNOV A A,TESEI G,et al.The effect of linker conformation on performance and stability of a two-domain lytic polysaccharide monooxygenase [J].Journal of Biological Chemistry,2023,299(11):105262.
[63] HUANG P H,CHIU C S,LU W C,et al.Change in chemical composition and enhancement of intestinal microflora of acid hydrolyzed polysaccharides from Zizyphus jujube and Sterculia lychnophora [J].Arabian Journal of Chemistry,2024,17(3):105598.
[64] DEMUTH T,BETSCHART J,NYSTRÖM L.Structural modifications to water-soluble wheat bran arabinoxylan through milling and extrusion [J].Carbohydrate Polymers,2020,240:116328.
[65] WANG Q,SHENG X J,SHI A M,et al.β-Glucans:relationships between modification,conformation and functional activities [J].Molecules,2017,22(2):257.
[66] RAHAYU R P,PRASETYO R A,PURWANTO D A,et al.The immunomodulatory effect of green tea (Camellia sinensis ) leaves extract on immunocompromised Wistar rats infected by Candida albicans [J].Veterinary World,2018,11(6):765-770.
[67] WANG B,GE L,MO J G,et al.Essential oils and ethanol extract from Camellia nitidissima and evaluation of their biological activity [J].Journal of Food Science and Technology,2018,55(12):5 075-5 081.
[68] YANG R,GUAN Y,WANG W X,et al.Antioxidant capacity of phenolics in Camellia nitidissima Chi flowers and their identification by HPLC Triple TOF MS/MS [J].PLoS One,2018,13(4):e0195508.
[69] SONG L X,WANG X S,ZHENG X Q,et al.Polyphenolic antioxidant profiles of yellow camellia [J].Food Chemistry,2011,129(2):351-357.[70] YANG R,GUAN Y,ZHOU J W,et al.Phytochemicals from Camellia nitidissima Chi flowers reduce the pyocyanin production and motility of Pseudomonas aeruginosa PAO 1[J].Frontiers in Microbiology,2018,8:2 640.
[71] CHEN J H,WU X H,ZHOU Y,et al.Camellia nitidissima Chi leaf as pancreatic lipase inhibitors:inhibition potentials and mechanism [J].Journal of Food Biochemistry,2021,45(9):e13837.
[72] CHEN Y W,ZHANG F,DU Z C,et al.Proteome analysis of Camellia nitidissima Chi revealed its role in colon cancer through the apoptosis and ferroptosis pathway [J].Frontiers in Oncology,2021,11:727130.
[73] HE X,LI H,ZHAN M X,et al.Camellia nitidissima chi extract potentiates the sensitivity of gastric cancer cells to paclitaxel via the induction of autophagy and apoptosis [J].OncoTargets and Therapy,2019,12:10 811-10 825.
[74] 韦霄,黄兴贤,蒋运生,等.3种金花茶组植物提取物的抗氧化活性比较 [J].中国中药杂志,2011,36(5):639-641.WEI X,HUANG X X,JIANG Y S,et al.Comparison of antioxidant activity of extracts from three plant groups of Camellia sinensis [J].China Journal of Chinese Materia Medica,2011,36(5):639-641.
[75] 邹登峰,张可锋,谢爱泽.金花茶多糖抗小鼠免疫性肝损伤作用的研究 [J].华西药学杂志,2014,29(5):525-527.ZOU D F,ZHANG K F,XIE A Z.Protective effect of Camellia nitidissima polysaccharides on immunological liver injury in the mice induced by BCG combined LPS [J].West China Journal of Pharmaceutical Sciences,2014,29(5):525-527.
[76] BAI Y,YE L,YANG K,et al.Genome survey and SSR analysis of Camellia nitidissima Chi (Theaceae ) [J].Genetics Research,2022,2 022(1):5417970.
[77] 黄思茂,曹后康,高雅,等.金花茶多糖对四氯化碳致小鼠急性肝损伤的保护作用及其机制的研究 [J].中药药理与临床,2016,32(6):117-120.HUANG S M,CAO H K,GAO Y,et al.Protective effect and mechanism of polysaccharides of Camellia nitidissima on acute hepatic injury induced by carbon tetrachloride in mice[J].Pharmacology and Clinics of Chinese Materia Medica,2016,32(6):117-120.
[78] 田宁,向文静,周紫贤,等.小胶质细胞在金花茶多糖保护脑出血中的作用 [J].中国药理学通报,2020,36(2):231-237.TIAN N,XIANG W J,ZHOU Z X,et al.Camellia nitidissima polysaccharides alleviate experimental intracerebral hemorrhage injury via regulating microglia M 1/M2 polarization [J].Chinese Pharmacological Bulletin,2020,36(2):231-237.