Enhancing the acidic activity of D-lyxose isomerase by molecular modification methods

Authors

LI Qiaoling, School of Food Science and Bioengineering , Changsha University of Science & Technology , Changsha , Hunan 410114 , China
HU Yang, School of Food Science and Bioengineering , Changsha University of Science & Technology , Changsha , Hunan 410114 , China
ZHOU Huiling, School of Food Science and Bioengineering , Changsha University of Science & Technology , Changsha , Hunan 410114 , China
YU Xun, School of Food Science and Bioengineering , Changsha University of Science & Technology , Changsha , Hunan 410114 , China
WEN Li, School of Food Science and Bioengineering , Changsha University of Science & Technology , Changsha , Hunan 410114 , China
WU Hao, School of Food Science and Bioengineering , Changsha University of Science & Technology , Changsha , Hunan 410114 , ChinaFollow

Corresponding Author(s)

吴昊（1990—），男，长沙理工大学副教授，博士。E-mail：haowu@csust.edu.cn

Abstract

［Objective］ To improve the activity of D-lyxose isomerase under acidic conditions.［Methods］ Solvent -accessible surface bioinformatics analysis was performed on surface residues of D-lyxose isomerase derived from Caldanaerobius polysaccharolyticus,and six mutation sites,i.e.,K2D,K2E,K8D,K8E,K18D,and K 18E,were designed.Mutants were constructed and subjected to recombinant inducible heterologous expression in Escherichia coli.The recombinant mutant enzymes were isolated and purified using a nickel affinity chromatography column.In vitro comparative experiments were conducted to evaluate the catalytic conversion of D-fructose to D-mannose by the recombinant mutant enzymes at acidic pH 5.5 and near -neutral pH 6.5.［Results］］ The recombinant mutant enzymes K 8D and K 8E showed significantly improved catalytic conversion rates of D-fructose,with the conversion rate reaching 140% under optimal conditions (pH 6.5).At pH 5.5,the conversion rate was 1.26 times higher than that of the wild -type enzyme.［Conclusion］ Molecular modification successfully enhanced the catalytic activity of D-lyxose isomerase mutants under acidic conditions.

Publication Date

6-2-2025

First Page

1

Last Page

7

DOI

10.13652/j.spjx.1003.5788.2025.60024

Recommended Citation

Qiaoling, LI; Yang, HU; Huiling, ZHOU; Xun, YU; Li, WEN; and Hao, WU (2025) "Enhancing the acidic activity of D-lyxose isomerase by molecular modification methods," Food and Machinery: Vol. 41: Iss. 4, Article 1.
DOI: 10.13652/j.spjx.1003.5788.2025.60024
Available at: https://www.ifoodmm.cn/journal/vol41/iss4/1

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