Abstract
［Objective］ To prepare Cordyceps militaris polypeptides using fermentation and analyze their antioxidant activity.［Methods］ The most suitable strain was screened from four candidates.Based on single -factor experiments and combined with orthogonal experiments,the fermentation process conditions of the selected strain were optimized.The polypeptide solution was separated and purified by ultrafiltration,and the antioxidant activity of C.militaris polypeptides was analyzed.［Results］］ The optimal conditions for preparing polypeptides from C.militaris protein by fermentation with Bacillus subtilis were as follows:3% addition of C.militaris protein,fermentation temperature of 36 ℃,inoculation amount of 4%,and fermentation time of 24 hours.Under these conditions,the polypeptide yield was 67.95%.After ultrafiltration,the fraction with a relative molecular mass <3 000 in the polypeptide solution exhibited the highest antioxidant activity.［Conclusion］ The process of preparing antioxidant peptides from C.militaris by fermentation with B.subtilis is feasible.
Publication Date
6-2-2025
First Page
148
Last Page
154
DOI
10.13652/j.spjx.1003.5788.2025.80019
Recommended Citation
Ting, XIN; Zhao, WANG; Changbin, LI; and Jian, ZOU
(2025)
"Optimization of the preparation process of Cordyceps militaris polypeptides through fermentation technology and evaluation of antioxidant activity,"
Food and Machinery: Vol. 41:
Iss.
4, Article 18.
DOI: 10.13652/j.spjx.1003.5788.2025.80019
Available at:
https://www.ifoodmm.cn/journal/vol41/iss4/18
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