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Corresponding Author(s)

秦礼康(1965—),男,贵州大学教授,博士生导师。E-mail:lkqin@gzu.edu.cn

Abstract

[Objective] To isolate and identify highly effective strains capable of removing chemical oxygen demand (COD ) and ammonia nitrogen from antibacterial wastewater.[Methods] Metagenomic sequencing technology is employed to deeply explore the microbial resources in wastewater isolated by freshly -extracted Zanthoxylum oil.The strains with the ability to efficiently remove COD and ammonia nitrogen are accurately analyzed.The strains with pollution -reducing potential are screened from wastewater enriched through natural outdoor cultivation for one month.[Results]] Proteobacteria and Firmicutes are the predominant phyla in the wastewater isolated by freshly -extracted Zanthoxylum oil,while Enterobacter and Acetobacter are the dominant genera.Three strains with significant COD and ammonia nitrogen removal capabilities are selected and identified as Bacillus stercoris strain X4,Pseudomonas putida X21,and Pseudomonas taiwanensis X22.In actual wastewater treatment,the COD removal rate of these three strains after 8 days is 55.47%,50.76%,and 52.32%,respectively,while the ammonia nitrogen removal rate after 36 hours is 55.93%,54.19%,and 40.79%,respectively.[Conclusion] The strains identified through indigenous enrichment in Zanthoxylum oil wastewater are more effective in reducing pollution than those from other sources.

Publication Date

6-2-2025

First Page

19

Last Page

26

DOI

10.13652/j.spjx.1003.5788.2025.80114

References

[1] DROZDOV I Y,ALEKSAKHIN A V,ALEKSAKHINA Y V,et al.Mathematical models of water pollution evaluation [J].IOP Conference Series:Earth and Environmental Science,2021,684(1):012026.
[2] 祁彦青,裔照晖.污水 COD超标的原因与控制研究 [J].工业微生物,2023,53(1):76-78.QI Y Q,YI Z H.Study on the causes and control of excessive COD in Sewage [J].Industrial Microbiology,2023,53(1):76-78.
[3] 邓永飞,刘涛,吴海铨,等.食品工业废水处理技术研究进展[J].工业水处理,2021,41(10):1-7,13.DENG Y F,LIU T,WU H Q,et al.Research progress of wastewater treatment technology in food industry [J].Industrial Water Treatment,2021,41(10):1-7,13.
[4] WU X L,ZHOU H,LI L Z,et al.Whole genome sequencing and comparative genomic analyses of Lysinibacillus pakistanensis LZH- 9,a halotolerant strain with excellent COD removal capability [J].Microorganisms,2020,8(5):716.
[5] 袁野,周佳,屈建航,等.高效反硝化聚磷菌的筛选及其脱氮除磷条件和性能研究 [J].生物技术通报,2023,39(7):266-276.YUAN Y,ZHOU J,QU J H,et al.Screening of an efficient denitrifying phosphorus-accumulating bacterium and its denitrification and phosphorus removal [J].Biotechnology Bulletin,2023 (7):266-276.
[6] 胡晴文,彭郁,李茉,等.花椒油和花椒籽油提取技术研究进展[J].中国油脂,2024,49(1):16-21.HU Q W,PENG Y,LI M,et al.Research progress on extraction process of Zanthoxylum bungeanum oil and Zanthoxylum bungeanum seed oil [J].China Oils and Fats,2024,49(1):16-21.
[7] 席少阳,郭延秀,马晓辉,等.花椒化学成分及药理作用的研究进展 [J].华西药学杂志,2021,36(6):717-722.XI S Y,GUO Y X,MA X H,et al.Research progress on chemical constituents and pharmacological effects of Zanthoxylum [J].West China Journal of Pharmaceutical Sciences,2021,36(6):717-722.
[8] 屈岩峰,郭晓婵,唐嘉瞳,等.花椒中天然成分提取工艺及在食品中的应用研究进展 [J].食品与机械,2024,40(5):234-240.QU Y F,GUO X C,TANG J T,et al.Research progress on the extraction process of natural components from Zanthoxylum bungeanumand its application in food [J].Food & Machinery,2024,40(5):234-240.
[9] LI D H,LIU C M,LUO R B,et al.MEGAHIT:an ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph [J].Bioinformatics,2015,31(10):1 674-1 676.
[10] LI D H,LUO R B,LIU C M,et al.MEGAHIT v 1.0:a fast and scalable metagenome assembler driven by advanced methodologies and community practices [J].Methods,2016,102:3-11.
[11] HYATT D,CHEN G L,LOCASCIO P F,et al.Prodigal:prokaryotic gene recognition and translation initiation site identification [J].BMC Bioinformatics,2010,11:119.
[12] BUCHFINK B,XIE C,HUSON D H.Fast and sensitive protein alignment using DIAMOND [J].Nature Methods,2015,12(1):59-60.
[13] 谭雨心,渡部由香,廖明系,等.柑橘皮油胞层提取物的抗氧化活性与抑菌活性 [J].食品与机械,2024,40(9):145-152.TAN Y X,WATANABE Y K,Liao M X,et al.Study on antioxidant and antibacterial activity of extracts from flavedo of citrus peel [J].Food & Machinery,2024,40(9):145-152.
[14] 梁红春,夏陈,邓俊琳,等.5种处理方法对赶黄草功效成分、体外抗氧化性和抑菌活性的影响 [J].食品与机械,2025,41(3):134-141.LIANG H C,XIA C,DENG J L,et al.Effects of five different processing methods on functional components,in vitro antioxidant ability,and antibacterial activities of Penthorum chinense Pursh [J].Food & Machinery,2025,41(3):134-141.
[15] WANG X h,XU X,BAO Z,et al.Analysis of fermentation microbial diversity in biogas slurry by using high-throughput sequencing in Xinjiang,China [J].Transactions of the Chinese Society of Agricultural Engineering,2019,35(5):219-225.
[16] HU X J.Microalgae-based meat processing wastewater treatment for nutrients recovery and water reconditioning [D].Lincoln:The University of Nebraska-Lincoln,2020.
[17] 赵婷婷,乔凯,王蕾,等.淀粉废水处理系统中活性污泥的微生物群落结构及多样性分析 [J].环境科学,2020,41(1):321-329.ZHAO T T,QIAO K,WANG L,et al.Measurements of bacterial community and biodiversity from activated sludge for a wastewater treatment containing starch [J].Environmental Science,2020,41(1):321-329.
[18] LI W,ZHU X Y,HOU Y H,et al.The treatment of high-concentration garlic processing wastewater by UASB-SBR [J].Environmental Technology,2023,44(7):921-935.
[19] ALEXYUK P,BOGOYAVLENSKIY A,ALEXYUK M,et al.Isolation and characterization of lytic bacteriophages active against clinical strains of E.coli and development of a phage antimicrobial cocktail [J].Viruses,2022,14(11):2 381.
[20] YANG W,CAI C,DAI X H.Interactions between virus surrogates and sewage sludge vary by viral analyte:recovery,persistence,and sorption [J].Water Research,2022,210:117995.
[21] AZZAM M,FAIESAL A A,MOHAMMED F A,et al.An innovative approach using lytic phage mix for wastewater management and pathogen control [J].Egyptian Journal of Applied Science,2023,38(3):14-44.
[22] ALVAREZ-PEREZ S,BAKER L J,MORRIS M M,et al.Acinetobacter pollinis sp.nov.,Acinetobacter baretiae sp.nov.and Acinetobacter rathckeae sp.nov.,isolated from floral nectar and honey bees [J].International Journal of Systematic and Evolutionary Microbiology,2021,71(5):4 783.
[23] DING Y F,HUANG C X,ZHU W J,et al.Characterization of a novel Jerseyvirus phage T 102 and its inhibition effect on biofilms of multidrug-resistant Salmonella [J].Virus Research,2023,326:199054.
[24] SYAZWANI A,MISNON M I,AHMAD M R,et al.The characteristics of acetobacter xylinum membrane affected by ph of culture medium [J].International Journal of Advanced Technology and Engineering Exploration,2019,9(1):5 873-5 878.
[25] SRIPHOCHANART W,KRUSONG W,SAMUELA N,et al.Enhancing small-scale acetification processes using adsorbed Acetobacter pasteurianus UMCC 2951 on κ-carrageenan-coated luffa sponge [J].PeerJ,2024,12:e17650.
[26] RAJALAKSHMI B S,FATHIMA A A S,JASMINE B S,et al.Pollutant removal from cheese processing effluent using effective indigenous natural scavengers [J].Environmental Monitoring and Assessment,2022,195(1):12.
[27] ZHANG Y X,XU Z X,LI J X,et al.Cooperation between two strains of Enterobacter and Klebsiella in the simultaneous nitrogen removal and phosphate accumulation processes [J].Bioresource Technology,2019,291:121854.
[28] 聂紫玉,吴艳阳,王增光,等.植物源益生乳酸菌的筛选及其特性 [J].食品科学,2022,43(18):143-151.NIE Z Y,WU Y Y,WANG Z G,et al.Screening and Characterization of Plant-derived Probiotic Lactic Acid Bacteria [J].Food Science,2022,43(18):143-151.
[29] 何正云,伍乔,毛雨,等.不同产地山胡椒油的抑菌活性比较研究 [J].山地农业生物学报,2025,44(1):51-58,75.HE Z Y,WU Q,MAO Y,et al.Comparative study on the antibacterial activities of litsea glauca oils from different geographical origins [J].Journal of Mountain Agriculture and Biology,2025,44(1):51-58,75.
[30] NEERACKAL G M,NDEGWA P M,JOO H S,et al.Potential application of Alcaligenes faecalis strain No.4 in mitigating ammonia emissions from dairy wastewater [J].Bioresource Technology,2016,206:36-42.
[31] 赵二劳,徐未芳,刘乐,等.花椒抑菌作用研究进展 [J].中国调味品,2019,44(3):185-188.ZHAO E L,XU W F,LIU L,et al.Research progress of the bacteriostasis of Zanthoxylum bungeanum [J].China Condiment,2019,44(3):185-188.
[32] 脱聪聪,丁旭,周瑫,等.甘肃陇南大红袍花椒芳香油成分分析 及 其 抑 菌 活 性 [J].食 品 工 业 科 技,2020,41(5):227-231,238.TUO C C,DING X,ZHOU T,et al.Composition analysis and antibacterial activity of aromatic oil of Dahongpao pepper from Longnan in Gansu Province [J].Science and Technology of Food Industry,2020,41(5):227-231,238.
[33] 王朝旭,任静.改性生物炭固定异养硝化菌对水中低浓度氨氮的去除 [J].环境污染与防治,2021,43(2):139-144.WANG Z X,REN J.Removal of ammonia nitrogen with low concentration in water by heterotrophic nitrifying bacteria immobilized on modified biochar [J].Environmental Pollution & Control,2021,43(2):139-144.
[34] HE T X,XIE D T,NI J P,et al.Nitrous oxide produced directly from ammonium,nitrate and nitrite during nitrification and denitrification [J].Journal of Hazardous Materials,2020,388:122114.
[35] PREM ANAND M,HARIHARAN V C,SHANKAR H,et al.Genome sequence of Bacillus subtilis subsp.subtilis Strain IITK SM 1,Isolated from kitchen waste compost [J].Microbiology Resource Announcements,2019,8(6):e01330 -18.
[36] 张云泽,张磊,张礼,等.水处理枯草芽孢杆菌的研究 [J].盐科学与化工,2022,51(2):15-17.ZHANG Y Z,ZHANG L,ZHANG L,et al.Study on Bacillus subtilisin water treatment [J].Journal of Salt Science and Chemical Industry,2022,51(2):15-17.

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