Research progress on methods for evaluating the effect of milk desensitization

Authors

XU Chenyu, College of Biotechnology and Food Science , Tianjin University of Commerce , Tianjin 300134 , China ;Institute of Collaborative Innovation in Great Health , Tianjin University of Commerce , Tianjin 300134 , China
LU Dingqiang, College of Biotechnology and Food Science , Tianjin University of Commerce , Tianjin 300134 , China ;Institute of Collaborative Innovation in Great Health , Tianjin University of Commerce , Tianjin 300134 , ChinaFollow
WANG Qian, College of Biotechnology and Food Science , Tianjin University of Commerce , Tianjin 300134 , China ;Institute of Collaborative Innovation in Great Health , Tianjin University of Commerce , Tianjin 300134 , China
LI Ming, College of Biotechnology and Food Science , Tianjin University of Commerce , Tianjin 300134 , China ;Institute of Collaborative Innovation in Great Health , Tianjin University of Commerce , Tianjin 300134 , China
WU Wenling, College of Biotechnology and Food Science , Tianjin University of Commerce , Tianjin 300134 , China ;Institute of Collaborative Innovation in Great Health , Tianjin University of Commerce , Tianjin 300134 , China
SONG Qingwei, College of Biotechnology and Food Science , Tianjin University of Commerce , Tianjin 300134 , China ;Institute of Collaborative Innovation in Great Health , Tianjin University of Commerce , Tianjin 300134 , China

Corresponding Author(s)

鲁丁强（1984—），男，天津商业大学副教授，硕士生导师，博士。E-mail：dqlu@tjcu.edu.cn

Abstract

Food allergies are becoming an increasingly serious issue,and milk,as one of the major allergens,severely affects the daily lives of individuals with allergies.This article reviews the recent research progress on methods for assessing the desensitization of milk allergenicity from the perspectives of protein structural changes,IgE/IgG binding capacity,animal sensitization models,in vitro digestion models,and cell degranulation assays.It also looks forward to the future directions of research on methods for evaluating the effectiveness of milk desensitization.

Publication Date

6-13-2025

First Page

176

Last Page

183

DOI

10.13652/j.spjx.1003.5788.2024.80926

Recommended Citation

Chenyu, XU; Dingqiang, LU; Qian, WANG; Ming, LI; Wenling, WU; and Qingwei, SONG (2025) "Research progress on methods for evaluating the effect of milk desensitization," Food and Machinery: Vol. 41: Iss. 5, Article 24.
DOI: 10.13652/j.spjx.1003.5788.2024.80926
Available at: https://www.ifoodmm.cn/journal/vol41/iss5/24

References

[1] SHARMA S,KUMAR P,BETZEL C,et al.Structure and function of proteins involved in milk allergies [J].Journal of Chromatography B:Biomedical Sciences and Applications,2001,756(1/2):183-187.
[2] NOCERINO R,BEDOGNI G,CARUCCI L,et al.The impact of formula choice for the management of pediatric cow's milk allergy on the occurrence of other allergic manifestations:the atopic march cohort study [J].The Journal of Pediatrics,2021,232:183-191.
[3] YANG M,TAN M Z,WU J L,et al.Prevalence,characteristics,and outcome of cow's milk protein allergy in Chinese infants:a population-based survey [J].Journal of Parenteral and Enteral Nutrition,2019,43(6):803-808.
[4] REGULA P,AGRESS A,ROSENSTREICH D,et al.Adult-onset IgE-mediated cow's milk allergy-a rare phenotype [J].The Journal of Allergy and Clinical Immunology Global,2023,2(4):100142.
[5] FLOM J D,SICHERER S H.Epidemiology of cow's milk allergy [J].Nutrients,2019,11(5):1 051.
[6] BALLEGAARD A R,BØGH K L.Intestinal protein uptake and IgE-mediated food allergy [J].Food Research International,2023,163:112150.
[7] LOZANO-OJALVO D,LEZMI G,CORTES-PEREZ N,et al.Non-IgE mediated food allergy [J].Drug Discovery Today:Disease Models,2015,17:45-53.
[8] PAPE S B.“May contain ” labelling:adequate consumer warning or unnecessarily defensive manufacturer behaviour ?[J].Journal of Consumer Policy,2009,32(2):165-188.
[9] CABANILLAS B,NOVAK N.Effects of daily food processing on allergenicity [J].Critical Reviews in Food Science and Nutrition,2019,59(1):31-42.
[10] PAN M F,YANG J Y,LIU K X,et al.Irradiation technology:an effective and promising strategy for eliminating food allergens [J].Food Research International,2021,148:110578.
[11] LIU C Q,SATHE S K.Food allergen epitope mapping [J].Journal of Agricultural and Food Chemistry,2018,66(28):7 238-7 248.
[12] YU X X,LIU M Q,LI X Y,et al.Qualitative and quantitative prediction of food allergen epitopes based on machine learning combined with in vitro experimental validation [J].Food Chemistry,2023,405:134796.
[13] LEPSKI S,BROCKMEYER J.Impact of dietary factors and food processing on food allergy [J].Molecular Nutrition & Food Research,2013,57(1):145-152.
[14] ZHOU F L,HE S D,SUN H J,et al.Advances in epitope mapping technologies for food protein allergens:a review [J].Trends in Food Science & Technology,2021,107:226-239.
[15] LAEMMLI U K.Cleavage of structural proteins during the assembly of the head of bacteriophage T 4[J].Nature,1970,227(5 259):680-685.
[16] HAGIWARA M.Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting analyses via colored stacking gels [J].Analytical Biochemistry,2022,652:114751.
[17] HAIDER S R,SHARP B L,REID H J.On-line coupling of gel electrophoresis and inductively coupled plasma-mass spectrometry [J].TrAC Trends in Analytical Chemistry,2011,30(11):1 793-1 808.
[18] PAGANO M.Application of electrophoresis and related methods,such as western blotting and zymography to the study of some proteins and enzymes [J].Analytica Chimica Acta,1999,383(1/2):119-125.
[19] DU X,YIN S L,WANG T,et al.Identification of proteolytic bacteria from Yunnan fermented foods and their use to reduce the allergenicity of β-lactoglobulin [J].Journal of Dairy Science,2024,107(11):8 990-9 004.
[20] GEETHU S,VIMINA E R.Protein secondary structure prediction using cascaded feature learning model [J].Applied Soft Computing,2023,140:110242.
[21] BONDUELLE C.Secondary structures of synthetic polypeptide polymers [J].Polymer Chemistry,2018,9(13):1 517-1 529.
[22] WANG K Q,SUN D W,PU H B,et al.Principles and applications of spectroscopic techniques for evaluating food protein conformational changes:a review [J].Trends in Food Science & Technology,2017, 67:207-219.
[23] FEVZIOGLU M,OZTURK O K,HAMAKER B R,et al.Quantitative approach to study secondary structure of proteins by FT-IR spectroscopy,using a model wheat gluten system [J].International Journal of Biological Macromolecules,2020,164:2 753-2 760.
[24] CHANG K F,LIU J B,JIANG W,et al.Ferulic acid-ovalbumin protein nanoparticles:structure and foaming behavior [J].Food Research International,2020,136:109311.
[25] SILIGARDI G,HUSSAIN R,PATCHING S G,et al.Ligand- and drug-binding studies of membrane proteins revealed through circular dichroism spectroscopy [J].Biochimica et Biophysica Acta (BBA )-Biomembranes,2014,1 838(1):34-42.
[26] STASZAK K,WIESZCZYCKA K,MARTURANO V,et al.Lanthanides complexes-chiral sensing of biomolecules [J].Coordination Chemistry Reviews,2019,397:76-90.
[27] ROGERS D M,JASIM S B,DYER N T,et al.Electronic circular dichroism spectroscopy of proteins [J].Chem,2019,5(11):2 751-2 774.
[28] VERDINO P,KELLER W.Circular dichroism analysis of allergens [J].Methods,2004,32(3):241-248.
[29] CHEN Y,TU Z C,WANG H,et al.Glycation of β-lactoglobulin under dynamic high pressure microfluidization treatment:effects on IgE-binding capacity and conformation[J].Food Research International,2016,89:882-888.
[30] SHAO Y H,ZHANG Y,ZHU M F,et al.Glycation of β-lactoglobulin combined by sonication pretreatment reduce its allergenic potential [J].International Journal of Biological Macromolecules,2020,164:1 527-1 535.
[31] EFTINK M R.Fluorescence techniques for studying protein structure [J].Methods of Biochemical Analysis,1991,35:127-205.
[32] ATOLE D M,RAJPUT H H.Ultraviolet spectroscopy and its pharmaceutical applications:a brief review [J].Asian Journal of Pharmaceutical and Clinical Research,2018,11(2):59.
[33] 杨庆,尚洁丽,曾浩龙,等.迷迭香酸共价偶联对 β-乳球蛋白结构及性质的影响 [J].食品科学,2024,45(11):61-67.YANG Q,SHANG J L,ZENG H L,et al.Effect of covalent coupling with rosmarinic acid on the structure and properties of β-lactoglobulin [J].Food Science,2024,45(11):61-67.
[34] RIDGLEY D M,CLAUNCH E C,LEE P W,et al.The role of protein hydrophobicity in conformation change and self-assembly into large amyloid fibers [J].Biomacromolecules,2014,15(4):1 240-1 247.
[35] KHODARAHMI R,SOORI H,AMANI M.Study of cosolvent-induced α-chymotrypsin fibrillogenesis:does protein surface hydrophobicity trigger early stages of aggregation reaction ?[J].The Protein Journal,2009,28(7):349-361.
[36] DASURI K,EBENEZER P,ZHANG L,et al.Increased protein hydrophobicity in response to aging and Alzheimer disease [J].Free Radical Biology and Medicine,2010,48(10):1 330-1 337.
[37] WITHANA-GAMAGE T S,HEGEDUS D D,MCINTOSH T C,et al.Subunit composition affects formation and stabilization of o/w emulsions by 11S seed storage protein cruciferin [J].Food Research International,2020,137:109387.
[38] LIU G X,TU Z C,YANG W H,et al.Investigation into allergenicity reduction and glycation sites of glycated β-lactoglobulin with ultrasound pretreatment by high-resolution mass spectrometry [J].Food Chemistry,2018,252:99-107.
[39] 杨子滢,葛新宇,闫欣,等.活性氧对牛乳 β-乳球蛋白结构、功 能 特 性 和 致 敏 性 的 影 响 [J].食 品 研 究 与 开 发,2023,44(21):18-25.YANG Z Y,GE X Y,YAN X,et al.Effects of reactive oxygen species on structure,functional properties and sensitization of bovine milk β-lactoglobulin [J].Food Research and Development,2023,44(21):18-25.
[40] OKAYAMA T,MATSUNO Y,YASUDA N,et al.Establishment of a quantitative ELISA for the measurement of allergen-specific IgE in dogs using anti-IgE antibody cross-reactive to mouse and dog IgE [J].Veterinary Immunology and Immunopathology,2011,139(2/3/4):99-106.[41] SINGH M M,SATIJA J.Enzyme-assisted metal nanoparticles etching based plasmonic ELISA:progress and insights [J].Analytical Biochemistry,2022,654:114820.
[42] TABATABAEI M S,ISLAM R,AHMED M.Applications of gold nanoparticles in ELISA,PCR,and immuno-PCR assays:a review [J].Analytica Chimica Acta,2021,1 143:250-266.
[43] XIONG Z Y,CHENG J F,HU Y X,et al.A composite enzyme derived from papain and chymotrypsin reduces the allergenicity of cow's milk allergen casein by targeting T and B cell epitopes [J].Food Chemistry,2024,459:140315.
[44] RODRÍGUEZ-CAMEJO C,DELFIN-RIELA T,ROSSOTTI M A,et al.A highly sensitive nanobody-based ELISA for bovine β-lactoglobulin to classified donated human milk destined to susceptible newborns [J].Food Control,2023,153:109910.
[45] HU Y Z,WANG Y,NIE L Q,et al.Exploration of specific nanobodies as immunological reagents to detect milk allergen of β-lactoglobulin without interference of hydrolytic peptides[J].Journal of Agricultural and Food Chemistry,2022,70(48):15 271-15 282.
[46] VANLANDSCHOOT P,STORTELERS C,BEIRNAERT E,et al.Nanobodies®:new ammunition to battle viruses [J].Antiviral Research,2011,92(3):389-407.
[47] MACIEJEWSKI E C,BASSO M A,MILLER C T,et al.The ethics of animal research and testing:a US perspective [J].Drug Discovery Today,2023,28(6):103545.
[48] MUKHERJEE P,ROY S,GHOSH D,et al.Role of animal models in biomedical research:a review [J].Laboratory Animal Research,2022,38(1):18.
[49] LIU C L,CHEN C,YAN X Y,et al.Assessment of immune responses and intestinal flora in BALB/c mice model of wheat food allergy via different sensitization methods [J].Food Science and Human Wellness,2023,12(3):871-881.
[50] HUANG J J,LIU C J,WANG Y B,et al.Application of in vitro and in vivo models in the study of food allergy [J].Food Science and Human Wellness,2018,7(4):235-243.
[51] LIU T G,NAVARRO S,LOPATA A L.Current advances of murine models for food allergy [J].Molecular Immunology,2016,70:104-117.
[52] MONMAI C,YOU S G,PARK W J.Immune-enhancing effects of anionic macromolecules extracted from Codium fragile on cyclophosphamide-treated mice [J].PLoS One,2019,14(2):e211570.
[53] REN H,ZHU X F,ZHAI S Y,et al.Seabuckthorn juice alleviates allergic symptoms in shrimp-induced food allergy mice [J].Food Science and Human Wellness,2023,12(3):783-788.
[54] HUANG M J,SHAO H M,ZHANG X,et al.Comparison of cow's milk allergy models highlighted higher humoral and Th 2 |immune responses in BALB/c than C 3H/HeNCrl mice [J].Food and Chemical Toxicology,2024,184:114315.
[55] HSIEH K Y,HSU C I,LIN J Y,et al.Oral administration of an edible-mushroom-derived protein inhibits the development of food-allergic reactions in mice [J].Clinical & Experimental Allergy,2003,33(11):1 595-1 602.
[56] STOJADINOVIC M,PIETERS R,SMIT J,et al.Cross-linking of β-lactoglobulin enhances allergic sensitization through changes in cellular uptake and processing [J].Toxicological Sciences,2014,140(1):224-235.
[57] LIANG X N,WANG Z Z,YANG H,et al.Evaluation of allergenicity of cow milk treated with enzymatic hydrolysis through a mouse model of allergy [J].Journal of Dairy Science,2022,105(2):1 039-1 050.
[58] BOISEN S,EGGUM B O.Critical evaluation of in vitro methods for estimating digestibility in simple-stomach animals[J].Nutrition Research Reviews,1991,4(1):141-162.
[59] MULET-CABERO A I,EGGER L,PORTMANN R,et al.A standardised semi-dynamic in vitro digestion method suitable for food-an international consensus [J].Food & Function,2020,11(2):1 702-1 720.
[60] COLES L T,MOUGHAN P J,DARRAGH A J.In vitro digestion and fermentation methods,including gas production techniques,as applied to nutritive evaluation of foods in the hindgut of humans and other simple-stomached animals [J].Animal Feed Science and Technology,2005,123:421-444.
[61] HUR S J,LIM B O,DECKER E A,et al.In vitro human digestion models for food applications [J].Food Chemistry,2011,125(1):1-12.
[62] HUR S J,LEE S J,KIM D H,et al.Onion extract structural changes during in vitro digestion and its potential antioxidant effect on brain lipids obtained from low- and high-fat-fed mice[J].Free Radical Research,2013,47(12):1 009-1 015.
[63] VERHOECKX K,BØGH K L,DUPONT D,et al.The relevance of a digestibility evaluation in the allergenicity risk assessment of novel proteins.Opinion of a joint initiative of COST action ImpARAS and COST action INFOGEST [J].Food and Chemical Toxicology,2019,129:405-423.
[64] EKMAY R D,COON C N,LADICS G S,et al.Allergenic potential of novel proteins-What can we learn from animal production ? [J].Regulatory Toxicology and Pharmacology,2017,89:240-243.
[65] LI Y W,XU R,XIU H N,et al.Effect of cinnamon on starch hydrolysis of rice pudding:comparing static and dynamic in vitro digestion models [J].Food Research International,2022,161:111813.
[66] DUPONT D,ALRIC M,BLANQUET-DIOT S,et al.Can dynamic in vitro digestion systems mimic the physiological reality ? [J].Critical Reviews in Food Science and Nutrition,2019,59(10):1 546-1 562.
[67] ZHAO Q R,WANG Y W,ZHU Z M,et al.Efficient reduction of β-lactoglobulin allergenicity in milk using Clostridium tyrobutyricum Z816[J].Food Science and Human Wellness,2023,12(3):809-816.
[68] ZHOU H L,TAN Y B,MCCLEMENTS D J.Applications of the INFOGEST in vitro digestion model to foods:a review [J].Annual Review of Food Science and Technology,2023,14:135-156.
[69] TORDESILLAS L,BERIN M C,SAMPSON H A.Immunology of food allergy [J].Immunity,2017,47(1):32-50.
[70] GE X Y,QU X,XIE C X,et al.The influence on the structure and allergenicity of milk β-lactoglobulin by methylglyoxal during thermal processing [J].Food Research International,2024,196:115043.
[71] PASSANTE E,FRANKISH N.The RBL- 2H3 cell line:its provenance and suitability as a model for the mast cell [J].Inflammation Research,2009,58(11):737-745.
[72] OKAHASHI N,NAKATA M,HIROSE Y,et al.Streptococcal H2O2 inhibits IgE-triggered degranulation of RBL- 2H3 mast cell/basophil cell line by inducing cell death [J].PLoS One,2020,15(4):e231101.
[73] YAMASHITA S,YOKOYAMA Y,HASHIMOTO T,et al.A novel in vitro co-culture model comprised of Caco- 2/RBL- 2H3 cells to evaluate anti-allergic effects of food factors through the intestine [J].Journal of Immunological Methods,2016,435:1-6.
[74] DING J,JU H P,ZHONG L M,et al.Reducing the allergenicity of pea protein based on the enzyme action of alcalase [J].Food & Function,2021,12(13):5 940-5 948.