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Corresponding Author(s)

李宁(1981—),女,河南农业大学教授,博士。E-mail:lining8028@126.com

Abstract

Quinolones (QNs ) are highly favored for their broad -spectrum antibacterial effects.However,the overuse of QNs in animal feed can lead to their residues entering the food chain,thus posing potential hazards to the environment and human health.This paper summarizes the main immunoassay techniques for quinolone residues,analyzes their advantages and application prospects,and discusses the challenges and future development directions of immunoassay techniques.

Publication Date

6-13-2025

First Page

216

Last Page

224

DOI

10.13652/j.spjx.1003.5788.2024.80944

References

[1] 曹刚.喹诺酮类抗菌药物杀菌的两条不同途径及其作用机制研究 [D].厦门:厦门大学,2019.CAO G.Two pathways and mechanisms underlying bactericidal action of quinolone antimicrobials [D].Xiamen:Xiamen University,2019.
[2] 包懿,刘斌,刘洋,等.食品中喹诺酮类药物残留检测方法的研究进展 [J].分析化学,2022,50(10):1 444-1 455.BAO Y,LIU B,LIU Y,et al.Research advances of detection methods for quinolones residues in foods [J].Chinese Journal of Analytical Chemistry,2022,50(10):1 444-1 455.
[3] 唐艳红,罗双群,杨梦冉,等.动物源性食品中抗生素残留、耐药细菌的分布及耐药基因的水平传播 [J].食品安全质量检测学报,2023,14(20):227-235.TANG Y H,LUO S Q,YANG M R,et al.Antibiotic residue,distribution of antibiotic resistant isolates and horizontal transfer of resistance genes in foods of animal origin [J].Journal of Food Safety & Quality,2023,14(20):227-235.
[4] 付海燕,卢欢欢,龙婉君,等.动物源食品中抗生素残留检测方法与研究进展 [J].轻工学报,2023,38(6):37-45.FU H Y,LU H H,LONG W J,et al.Progress in the detection methods of antibiotic residues in animal derived food and research [J].Journal of Light Industry,2023,38(6):37-45.
[5] 王翠月,陈大伟,马丽娜,等.动物源性食品中氟喹诺酮类药物残留现状和检测方法研究进展 [J].中国家禽,2022,44(12):92-97.WANG C Y,CHEN D W,MA L N,et al.Research progress of current status and detection methods of fluoroquinolones in animal derived food [J].China Poultry,2022,44(12):92-97.
[6] QI J,FAN X X,DENG D M,et al.Progress in rapid detection techniques using paper-based platforms for food safety [J].Chinese Journal of Analytical Chemistry,2020,48(12):1 616-1 624.
[7] CHENG Y,WANG H L,ZHUO Y X,et al.Reusable smartphone-facilitated mobile fluorescence biosensor for rapid and sensitive on-site quantitative detection of trace pollutants [J].Biosensors and Bioelectronics,2022,199:113863.
[8] 李倩,王甲,张玉洁,等.动物性食品中喹诺酮类药物残留检测 方 法 研 究 进 展 [J].食 品 安 全 质 量 检 测 学 报,2021,12(8):3 016-3 022.LI Q,WANG J,ZHANG Y J,et al.Research progress on determination methods of quinolone residues in animal food [J].Journal of Food Safety & Quality,2021,12(8):3 016-3 022.
[9] 刘志会,陈文静.食品中喹诺酮类药物残留检测方法的研究进展 [J].现代食品,2023,29(8):160-162.LIU Z H,CHEN W J.Research progress on detection methods for quinolone residues in food [J].Modern Food,2023,29(8):160-162.
[10] 王宇宸.畜禽肉中氟喹诺酮残留免疫层析快速检测方法的建立及应用 [D].南昌:南昌大学,2023.WANG Y C.Establishment and application of rapid immunochromatographic methods for the detection of |fluoroquinolone residues in livestock and poultry meat [D].Nanchang:Nanchang University,2023.
[11] 桑 晓 霞,马 江 媛,黄 登 宇.赭 曲 霉 毒 素 A检 测 方 法 的 研 究 进展[J].食品安全质量检测学报,2019,10(21):7 271-7 277.SANG X X,MA J Y,HUANG D Y.Research progress on detection methods of ochratoxin A [J].Journal of Food Safety & Quality,2019,10(21):7 271-7 277.
[12] XING K Y,SHAN S,LIU D F,et al.Recent advances of lateral flow immunoassay for mycotoxins detection [J].TrAC Trends in Analytical Chemistry,2020,133:116087.
[13] MUKHAMETOVA L I,EREMIN S A.Fluorescence polarization assays for organic compounds in food safety [J].Frontiers in Bioscience,2024,16(1):4.
[14] ZHOU H L,LI X D,WANG L H,et al.Application of SERS quantitative analysis method in food safety detection [J].Reviews in Analytical Chemistry,2021,40(1):173-186.
[15] XIAO Q,XU C X.Research progress on chemiluminescence immunoassay combined with novel technologies [J].TrAC Trends in Analytical Chemistry,2020,124:115780.
[16] WANG K,LIN X G,ZHANG M X,et al.Review of electrochemical biosensors for food safety detection [J].Biosensors,2022,12(11):959.
[17] PAN Y T,YANG H J,WEN K,et al.Current advances in immunoassays for quinolones in food and environmental samples [J].TrAC Trends in Analytical Chemistry,2022,157:116726.
[18] THURNER F,ALATRAKTCHI F A.Recent trends in biosensors for quinolone detection:a comprehensive review [J].Chemosensors,2023,11(9):493.
[19] ACAROZ U,DIETRICH R,KNAUER M,et al.Development of a generic enzyme-immunoassay for the detection of fluoro(quinolone )-residues in foodstuffs based on a highly sensitive monoclonal antibody [J].Food Analytical Methods,2020,13(3):780-792.
[20] WANG F Y,LI N,ZHANG Y S,et al.Preparation and directed evolution of anti-ciprofloxacin ScFv for immunoassay in animal-derived food [J].Foods,2021,10(8):1 933.
[21] 罗梅霞.基于免疫层析法快速检测肉类中氟喹诺酮的研究[D].南昌:南昌大学,2022.LUO M X.Research on rapid detection of fluoroquinolones in meat based on immunochromatographic assay [D].Nanchang:Nanchang University,2022.
[22] HU S,HUANG Z,WANG C,et al.Using hapten cross-reactivity to screen heterologous competitive antigens for improving the sensitivity of ELISA [J].Food Chemistry,2020,303:125379.
[23] MAHMOUDI T,DE LA GUARDIA M,SHIRDEL B,et al.Recent advancements in structural improvements of lateral flow assays towards point-of-care testing [J].TrAC Trends in Analytical Chemistry,2019,116:13-30.
[24] 杨娅林,陆彦蓉,殷晓阳,等.基于纳米材料的免疫层析试纸在 兽 药 残 留 检 测 中 的 应 用 [J].中 国 兽 药 杂 志,2022,56(10):60-68.YANG Y L,LU Y R,YIN X Y,et al.Application of immunochromatographic assay strip based on nanomaterials in veterinary drug residues detection [J].Chinese Journal of Veterinary Drug,2022,56(10):60-68.
[25] XUE G J,WU M Y,LIU T T,et al.A multiple lateral flow immunoassay based on AuNP for the detection of 5 chemical contaminants in milk [J].Journal of Dairy Science,2023,106(6):3 856-3 867.
[26] LIU C,YANG L Y,ZHANG W,et al.A magnetic nanoparticle-based lateral flow immunochromatography assay for the rapid detection of fluoroquinolones in milk [J].European Food Research and Technology,2021,247(10):2 645-2 656.
[27] BAO H H,YUAN M F,XIAO C G,et al.Development of a signal-enhanced LFIA based on tyramine-induced AuNPs aggregation for sensitive detection of danofloxacin [J].Food Chemistry,2022,375:131875.
[28] XIAO J X,YANG H F,QIN L N,et al.Rapid detection of fluoroquinolone residues in aquatic products based on a gold-labeled microwell immunochromatographic assay [J].Food Quality and Safety,2022,610:1 093.
[29] HENDRICKSON O D,ZVEREVA E A,SHANIN I A,et al.Development of a multicomponent immunochromatographic test system for the detection of fluoroquinolone and amphenicol antibiotics in dairy products [J].Journal of the Science of Food and Agriculture,2019,99(8):3 834-3 842.
[30] 王雪晴,陈秀金,李兆周,等.氟喹诺酮类药物免疫分析方法的研究进展 [J].食品与发酵工业,2024,50(7):374-382.WANG X Q,CHEN X J,LI Z Z,et al.Research progress on immunoassays for determination of fluoroquinolone residue [J].Food and Fermentation Industries,2024,50(7):374-382.
[31] GUO L C,LIU M X,LI Q,et al.Synthesis and characterization of tracers and development of a fluorescence polarization immunoassay for amantadine with high sensitivity in chicken [J].Journal of Food Science,2021,86(10):4 754-4 767.
[32] LIU Y W,LUO Y B,LI W X,et al.Current analytical strategies for the determination of quinolone residues in milk[J].Food Chemistry,2024,430:137072.
[33] JIN Q S,FAN Y H,HE T,et al.Fluorescence polarization assay based on a new recognition motif QepA for the one-step detection of fluoroquinolones in eggs [J].Journal of Agricultural and Food Chemistry,2023,71(49):19 749-19 759.
[34] EL KOJOK H,EL DARRA N,KHALIL M,et al.Fluorescence polarization assay to detect the presence of traces of ciprofloxacin [J].Scientific Reports,2020,10(1):4 550.
[35] SHEN X,CHEN J H,LV S W,et al.Fluorescence polarization immunoassay for determination of enrofloxacin in pork liver and chicken [J].Molecules,2019,24(24):4 462.
[36] 徐婧,郑红,谢丽芳,等.鸡肉样品中痕量喹诺酮类抗生素的表 面 增 强 拉 曼 光 谱 检 测 研 究 [J].分 析 化 学,2023,51(3):397-418.XU J,ZHENG H,XIE L F,et al.Fast detection of trace enrofloxacin and ciprofloxacin in chicken meat by surface-enhanced Raman spectroscopy [J].Chinese Journal of Analytical Chemistry,2023,51(3):397-418.
[37] SHI Q Q,HUANG J,SUN Y N,et al.A SERS-based multiple immuno-nanoprobe for ultrasensitive detection of neomycin and quinolone antibiotics via a lateral flow assay [J].Mikrochimica Acta,2018,185(2):84.
[38] ZHANG H Z,TANG Y M,WANG W E,et al.A new semiconductor heterojunction SERS substrate for ultra-sensitive detection of antibiotic residues in egg [J].Food Chemistry,2024,431:137163.
[39] LIU J,LIU W,ZHOU S N,et al.Free-standing membrane liquid-state platform for SERS-based determination of norfloxacin in environmental samples [J].Journal of Analysis and Testing,2021,5(3):217-224.
[40] 向国庆,连聪,孙栋,等.化学发光免疫分析法及在动物生产领 域 检 测 中 的 应 用 进 展 [J].中 国 兽 医 科 学,2023,53(10):1 320-1 325.XIANG G Q,LIAN C,SUN D,et al.Progress of chemiluminescence immunoassay and its application in the detection of animal production [J].Chinese Veterinary Science,2023,53(10):1 320-1 325.
[41] PEI Y Q,ZENG L J,WEN C F,et al.Detection of enrofloxacin by flow injection chemiluminescence immunoassay based on cobalt hydroxide nanozyme [J].Mikrochimica Acta,2021,188(6):194.
[42] HAN Z Y,SUN T Q,XU Z H,et al.Detection of 4 quinolone antibiotics by chemiluminescence based on a novel Nor-Biotin bifunctional ligand and SA-ALP technology [J].Bioscience,Biotechnology,and Biochemistry,2021,85(7):1 720-1 728.
[43] VAKH C,POCHIVALOV A,KORONKIEWICZ S,et al.A chemiluminescence method for screening of fluoroquinolones in milk samples based on a multi-pumping flow system [J].Food Chemistry,2019,270:10-16.
[44] KAYA H O,CETIN A E,AZIMZADEH M,et al.Pathogen detection with electrochemical biosensors:advantages,challenges and future perspectives [J].Journal of Electroanalytical Chemistry,2021,882:114989.
[45] LAMARCA R S,DE FARIA R A D,ZANONI M V B,et al.Simple,fast and environmentally friendly method to determine ciprofloxacin in wastewater samples based on an impedimetric immunosensor [J].RSC Advances,2020,10(4):1 838-1 847.
[46] CHEN W L,ZHOU R,YAO X,et al.Sensitive detection of enrofloxacin using an electrochemiluminescence immunosensor based on gold-functionalized C60 and Au@BSA nanoflowers [J].New Journal of Chemistry,2018,42(17):14 142-14 148.
[47] LIU B,LI M,ZHAO Y S,et al.A sensitive electrochemical immunosensor based on PAMAM dendrimer-encapsulated Au for detection of norfloxacin in animal-derived foods [J].Sensors,2018,18(6):1 946.
[48] AYMARD C,KANSO H,SERRANO M J,et al.Development of a new dual electrochemical immunosensor for a rapid and sensitive detection of enrofloxacin in meat samples [J].Food Chemistry,2022,370:131016.
[49] 仇彩霞,陈秀金,付祖耀,等.基于石墨烯—壳聚糖复合膜修饰 电 极 的 麻 保 沙 星 电 化 学 免 疫 传 感 器 的 构 建 [J].食 品 与 发酵工业,2022,48(16):50-55.QIU C X,CHEN X J,FU Z Y,et al.Establishment of an electrochemical immunosensor for marbofloxacin analysis based on graphene oxide-chitosan nanocomposite film modified glass carbon electrode [J].Food and Fermentation Industries,2022,48(16):50-55.
[50] JIAO M Q,ZHANG J X,WU K,et al.A novel electrochemiluminescence immunosensing strategy fabricated by Co (OH)2 two-dimensional nanosheets and Ru@SiO2-Au NPs for the highly sensitive detection of enrofloxacin [J].The Analyst,2021,146(17):5 429-5 436.
[51] CHAUDHARY N,YADAV A K,SHARMA J G,et al.Designing and characterization of a highly sensitive and selective biosensing platform for ciprofloxacin detection utilizing lanthanum oxide nanoparticles [J].Journal of Environmental Chemical Engineering,2021,9(6):106771.
[52] BIZINA E V,FARAFONOVA O V,ZOLOTAREVA N I,et al.A piezoelectric immunosensor based on magnetic carbon nanocomposites for the determination of ciprofloxacin [J].Journal of Analytical Chemistry,2022,77(4):458-465.
[53] HAMMAMI I,ALABDALLAH N M,AL JOMAA A,et al.Gold nanoparticles:synthesis properties and applications [J].Journal of King Saud University-Science,2021,33(7):101560.
[54] ARSHAVSKY-GRAHAM S,HEUER C,JIANG X,et al.Aptasensors versus immunosensors:which will prevail ? [J].Engineering in Life Sciences,2022,22(3/4):319-333.
[55] TUERK C,GOLD L.Systematic evolution of ligands by exponential enrichment:RNA ligands to bacteriophage T 4 DNA polymerase [J].Science,1990,249(4 968):505-510.
[56] TAGHDISI HEIDARIAN S M,TAVANAEE SANI A,DANESH N M,et al.A novel electrochemical approach for the ultrasensitive detection of fluoroquinolones based on a double-labelled aptamer to surpass complementary strands of aptamer lying flat [J].Sensors and Actuators B:Chemical,2021,334:129632.
[57] WANG M H,HU M Y,LIU J M,et al.Covalent organic framework-based electrochemical aptasensors for the ultrasensitive detection of antibiotics [J].Biosensors and Bioelectronics,2019,132:8-16.
[58] BAO X L,WANG S Y,HAO Q F,et al.Enrofloxacin rapid detection in aquatic foods:based on DNA aptamer sensor [J].Foods,2024,13(6):941.

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