Immobilization of bromelain on Fe₃O₄-SA/CS microspheres and its enzymatic properties

Authors

WANG Dan, Muhammad Zain Ul Aabideen1,2 Mbinga Isequias Elamba Tertulliano1,2 ZHOU Wei3（College of Food and Health , Jinzhou Medical University , Jinzhou , Liaoning 121000 , China ;Liaoning Province Meat Processing and Quality Safety Control Professional Technology Innovation Center , Jinzhou , Liaoning 121000 , China
GENG Lijing, Muhammad Zain Ul Aabideen1,2 Mbinga Isequias Elamba Tertulliano1,2 ZHOU Wei3（College of Food and Health , Jinzhou Medical University , Jinzhou , Liaoning 121000 , China ;Liaoning Province Meat Processing and Quality Safety Control Professional Technology Innovation Center , Jinzhou , Liaoning 121000 , ChinaFollow
HUANG Wei, Muhammad Zain Ul Aabideen1,2 Mbinga Isequias Elamba Tertulliano1,2 ZHOU Wei3（College of Food and Health , Jinzhou Medical University , Jinzhou , Liaoning 121000 , China ;Liaoning Province Meat Processing and Quality Safety Control Professional Technology Innovation Center , Jinzhou , Liaoning 121000 , China
FU Hang, Muhammad Zain Ul Aabideen1,2 Mbinga Isequias Elamba Tertulliano1,2 ZHOU Wei3（College of Food and Health , Jinzhou Medical University , Jinzhou , Liaoning 121000 , China ;Liaoning Province Meat Processing and Quality Safety Control Professional Technology Innovation Center , Jinzhou , Liaoning 121000 , China
LIU Xiaoyu, Muhammad Zain Ul Aabideen1,2 Mbinga Isequias Elamba Tertulliano1,2 ZHOU Wei3（College of Food and Health , Jinzhou Medical University , Jinzhou , Liaoning 121000 , China ;Liaoning Province Meat Processing and Quality Safety Control Professional Technology Innovation Center , Jinzhou , Liaoning 121000 , China

Corresponding Author(s)

耿丽晶（1981—），女，锦州医科大学教授，博士。E-mail：gengli777@126.com

Abstract

［Objective］ To improve the immobilization technology of bromelain which has poor stability to enhance its utilization,stability,and storage performance.［Methods］ The preparation process of magnetic chitosan/sodium alginate immobilized bromelain (Fe3O4-SA/CS -Br) is optimized by the response surface methodology.The Fe3O4-SA/CS -Br is characterized and its Km value,operational stability,thermal stability,storage stability,and application effectiveness are determined.［Results］ The optimal preparation conditions for Fe3O4-SA/CS -Br are as follows:mchitosan∶msodium alginate ratio of 1∶4,Fe3O4 mass concentration of 10 mg/mL,CaCl2 mass concentration of 32 mg/mL,immobilization time of 106 min,pH value of 7.0,and enzyme mass concentration of 1.0 mg/mL.According to the results from scanning electron microscopy,infrared spectrum,and X -ray diffraction spectrum,the main functional groups of bromelain are successfully immobilized on Fe3O4-chitosan/sodium alginate (Fe3O4-SA/CS ) microspheres.The affinity of bromelain for the substrate is enhanced after immobilization.引用格式：王丹，耿丽晶，黄薇，等.Fe3O4-SA/CS 微球固定菠萝蛋白酶及其酶学性质研究 ［J］.食品与机械，2025，41（5）：27-35.C itation:WANG Dan,GENG Lijing,HUANG Wei,et al.Immobilization of bromelain on Fe3O4-SA/CS microspheres and its enzymatic properties [J].Food & Machinery,2025,41(5):27-35.［Conclusion］ Fe3O4-SA/CS -Br can effectively enhance the stability of bromelain.

Publication Date

6-13-2025

First Page

27

Last Page

35

DOI

10.13652/j.spjx.1003.5788.2024.80912

Recommended Citation

Dan, WANG; Lijing, GENG; Wei, HUANG; Hang, FU; and Xiaoyu, LIU (2025) "Immobilization of bromelain on Fe₃O₄-SA/CS microspheres and its enzymatic properties," Food and Machinery: Vol. 41: Iss. 5, Article 4.
DOI: 10.13652/j.spjx.1003.5788.2024.80912
Available at: https://www.ifoodmm.cn/journal/vol41/iss5/4

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