Metabolic engineering of Saccharomyces cerevisiae for de novo biosynthesis of naringin from D-xylose

Authors

ZHAO Yifei, Longping Branch Graduate School , Hunan University , Changsha , Hunan 410125 , China;Dong Ting Laboratory , Hunan Institute of Agricultural Product Processing and Quality Safety , Changsha , Hunan 410125 , China
LI Gaoyang, Longping Branch Graduate School , Hunan University , Changsha , Hunan 410125 , China;Dong Ting Laboratory , Hunan Institute of Agricultural Product Processing and Quality Safety , Changsha , Hunan 410125 , China;Hunan Academy of Agricultural Sciences , Changsha , Hunan 410125 , ChinaFollow
HUANG Luhong, Dong Ting Laboratory , Hunan Institute of Agricultural Product Processing and Quality Safety , Changsha , Hunan 410125 , China;Hunan Academy of Agricultural Sciences , Changsha , Hunan 410125 , China
LIU Juan, Dong Ting Laboratory , Hunan Institute of Agricultural Product Processing and Quality Safety , Changsha , Hunan 410125 , China;Hunan Academy of Agricultural Sciences , Changsha , Hunan 410125 , China
XIAO Zhiqiang, Longping Branch Graduate School , Hunan University , Changsha , Hunan 410125 , China;Dong Ting Laboratory , Hunan Institute of Agricultural Product Processing and Quality Safety , Changsha , Hunan 410125 , China

Corresponding Author(s)

李高阳（1971—），男，湖南大学研究员，博士生导师，博士。E-mail：lgy7102@163.com

Abstract

［Objective］ To explore the biosynthesis method of naringin,a bioactive compound in citrus.［Methods］ Engineered Saccharomyces cerevisiae strains for naringenin biosynthesis are constructed through enzyme screening,multi -copy integration,and precursor supply.Subsequently,de novo biosynthesis of naringin from D-xylose is achieved by introducing the naringin biosynthetic pathway and optimizing UDP -glucose supply.［Results］］ The optimal flavonoid synthase is identified as PhCHS by screening the key enzymes of the naringenin pathway.The naringenin biosynthesis pathway is further integrated at multi -copy sites.The constructed strain Z39M2 can synthesize 36.53 mg/L naringenin.The overexpression of CAT 2,xPK-PTA pathway,and ACC 1m optimizes malonyl -CoA supply and constructs strain Z 44,with a naringenin production increasing to 72.96 mg/L.Finally,the naringin biosynthesis pathway introduction enables the successful biosynthesis of 35.60 mg/L naringin.Further overexpression of PGM 1 and UGP 1 elevates naringin production to 76.67 mg/L.［Conclusion］ An engineered S.cerevisiae strain capable of de novo biosynthesis of naringin from D-xylose is successfully constructed by metabolic engineering.

Publication Date

7-3-2025

First Page

1

Last Page

8

DOI

10.13652/j.spjx.1003.5788.2025.80274

Recommended Citation

Yifei, ZHAO; Gaoyang, LI; Luhong, HUANG; Juan, LIU; and Zhiqiang, XIAO (2025) "Metabolic engineering of Saccharomyces cerevisiae for de novo biosynthesis of naringin from D-xylose," Food and Machinery: Vol. 41: Iss. 6, Article 1.
DOI: 10.13652/j.spjx.1003.5788.2025.80274
Available at: https://www.ifoodmm.cn/journal/vol41/iss6/1

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