Abstract
［Objective］ To develop a label -free and visual colorimetric assay for the rapid detection of bisphenol A (BPA ) using a BPA aptamer as the recognition molecule and metal -organic framework (Fe-MIL -101) nanozyme mimics as the signal probe.［Methods］ The BPA aptamer was added to the Fe -MIL -101 solution,where it was adsorbed onto the Fe -MIL -101 surface through electrostatic interactions,thereby inhibiting the activity of nanozyme mimics.In the presence of BPA in the test sample,the aptamer specifically binds to BPA and detaches from the Fe -MIL -101 surface,restoring the catalytic activity of Fe -MIL -101.This enables Fe -MIL -101 to catalyze a colorless peroxidase substrate,generating a colorimetric output signal.The qualitative and quantitative analysis of BPA was achieved by monitoring the changes in the colorimetric signal.［Results］］ Under optimized conditions,the linear detection range for BPA in a buffered solution was 1~80 ng/mL,with a naked -eye visual detection limit of 0.5 ng/mL.When applied to BPA detection in milk and milk powder,the method achieved spike recoveries ranging from 83.6% to 95.2%,with relative standard deviations (RSDs ) between 2.96% and 7.45%.［Conclusion］ This method is simple,rapid,and highly accurate,demonstrating strong potential for practical applications.
Publication Date
7-3-2025
First Page
88
Last Page
95
DOI
10.13652/j.spjx.1003.5788.2024.80973
Recommended Citation
Li, JIANG; Chunxia, LU; Xiangqun, LIN; and Xia, CHEN
(2025)
"Colorimetric detection of bisphenol A in milk and milk powder based on label-free aptamer and metal-organic framework enzyme mimics,"
Food and Machinery: Vol. 41:
Iss.
6, Article 13.
DOI: 10.13652/j.spjx.1003.5788.2024.80973
Available at:
https://www.ifoodmm.cn/journal/vol41/iss6/13
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