Effect of UPLC-MS/MS pre-column derivatization on ergosterol content in edible fungi

Authors

ZHANG Xinyue, College of Life Sciences , China Jiliang University , Hangzhou , Zhejiang 310018 , China;Zhejiang Key Laboratory of Intelligent Food Logistics and Processing , Hangzhou , Zhejiang 310021 , China;Key Laboratory of Post-harvest Handling of Fruits , Ministry of Agriculture and Rural Affairs , Hangzhou , Zhejiang 310021 , China;Food Science Institute , Zhejiang Academy of Agricultural Sciences , Hangzhou , Zhejiang 310021 , China
CHEN Hangjun, Zhejiang Key Laboratory of Intelligent Food Logistics and Processing , Hangzhou , Zhejiang 310021 , China;Key Laboratory of Post-harvest Handling of Fruits , Ministry of Agriculture and Rural Affairs , Hangzhou , Zhejiang 310021 , China;Food Science Institute , Zhejiang Academy of Agricultural Sciences , Hangzhou , Zhejiang 310021 , China
NIU Ben, Zhejiang Key Laboratory of Intelligent Food Logistics and Processing , Hangzhou , Zhejiang 310021 , China;Key Laboratory of Post-harvest Handling of Fruits , Ministry of Agriculture and Rural Affairs , Hangzhou , Zhejiang 310021 , China;Food Science Institute , Zhejiang Academy of Agricultural Sciences , Hangzhou , Zhejiang 310021 , China
SHENTU Xuping, College of Life Sciences , China Jiliang University , Hangzhou , Zhejiang 310018 , China
YANG Hailong, School of Life and Environmental Sciences , Wenzhou University , Wenzhou , Zhejiang 325035 , China
YIN Ming, Zhejiang Key Laboratory of Intelligent Food Logistics and Processing , Hangzhou , Zhejiang 310021 , China;Key Laboratory of Post-harvest Handling of Fruits , Ministry of Agriculture and Rural Affairs , Hangzhou , Zhejiang 310021 , China;Food Science Institute , Zhejiang Academy of Agricultural Sciences , Hangzhou , Zhejiang 310021 , China
GAO Haiyan, Zhejiang Key Laboratory of Intelligent Food Logistics and Processing , Hangzhou , Zhejiang 310021 , China;Key Laboratory of Post-harvest Handling of Fruits , Ministry of Agriculture and Rural Affairs , Hangzhou , Zhejiang 310021 , China;Food Science Institute , Zhejiang Academy of Agricultural Sciences , Hangzhou , Zhejiang 310021 , China
MU Honglei, Zhejiang Key Laboratory of Intelligent Food Logistics and Processing , Hangzhou , Zhejiang 310021 , China;Key Laboratory of Post-harvest Handling of Fruits , Ministry of Agriculture and Rural Affairs , Hangzhou , Zhejiang 310021 , China;Food Science Institute , Zhejiang Academy of Agricultural Sciences , Hangzhou , Zhejiang 310021 , ChinaFollow

Corresponding Author(s)

穆宏磊（1980—），男，浙江省农业科学院研究员，博士。E-mail：mhljoe@l63.com

Abstract

［Objective］ To improve the responsiveness of ergosterol in electrospray ionization mode and to investigate the ergosterol derivatization through dansylation.［Methods］ With dansyl chloride as the derivatizing agent and 4-dimethylaminopyridine (DMAP ) as the catalyst,this paper carries out a response surface test to optimize derivatization conditions.The samples are separated on a C18 column utilizing isocratic elution with a 0.15% formic acid -methanol solution as the mobile phase.Multiple reaction monitoring is performed using an ESI source.The ergosterol content in 10 edible fungi is assessed.［Results］］ The optimal derivatization conditions are the reaction temperature of 36 ℃,the reaction time of 25 min,and the DMAP mass concentration of 8 µg/mL.The derivatized ergosterol exhibits a broad 郜海燕（1958—），女，浙江省农业科学院研究员，博士。E-mail：spsghy@l 63.com引用格式：张新月，陈杭君，牛犇，等.UPLC -MS/MS柱前衍生对食用菌中麦角甾醇含量的影响 ［J］.食品与机械，2025，41（6）：36-43.C itation:ZHANG Xinyue,CHEN Hangjun,NIU Ben,et al.Effect of UPLC-MS/MS pre-column derivatization on ergosterol content in edible fungi [J].Food & Machinery,2025,41(6):36-43.|linearity range of 25~2 000 ng/mL.The detection limit is 13.4 ng/mL,while the quantification limit is 149 ng/mL,with the sensitivity 51.94 times higher than the pre -derivatization state.Among the 10 edible fungi,shiitake mushrooms exhibit the highest ergosterol content,which is (4.21±0.083) mg/g.［Conclusion］ This method is characterized by its ease of operation,high sensitivity,and accuracy.

Publication Date

7-3-2025

First Page

36

Last Page

43

DOI

10.13652/j.spjx.1003.5788.2025.80170

Recommended Citation

Xinyue, ZHANG; Hangjun, CHEN; Ben, NIU; Xuping, SHENTU; Hailong, YANG; Ming, YIN; Haiyan, GAO; and Honglei, MU (2025) "Effect of UPLC-MS/MS pre-column derivatization on ergosterol content in edible fungi," Food and Machinery: Vol. 41: Iss. 6, Article 5.
DOI: 10.13652/j.spjx.1003.5788.2025.80170
Available at: https://www.ifoodmm.cn/journal/vol41/iss6/5

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