Abstract
［Objective］ To explore the antioxidant activity and molecular mechanism of the typical flavonoid apigenin and its glycoside compounds in Dendrobium officinale.［Methods］ The antioxidant activities of apigenin and its glycoside compounds were assessed using the DPPH free radical scavenging assay,and their effects on the expression of antioxidant proteins in HepG 2 cells were examined by Western blot.［Results］］ Vitexin exhibited significantly stronger antioxidant activity than apigenin and New Zealand vitexin Ⅱ,with an IC50 value for DPPH radical scavenging of 43.48 mmol/L.MTT assays confirmed that apigenin (20 μmol/L ),vitexin,and New Zealand vitexin Ⅱ (80 μmol/L ) were non -toxic to HepG 2 cells.In normal HepG 2 cells,5 μmol/L apigenin significantly upregulated the expression of the antioxidant proteins Nrf 2 and NQO 1 compared with its glycoside compounds.In the t -BHP -induced oxidative stress model,apigenin significantly alleviated oxidative damage by promoting nuclear translocation of Nrf 2,activating the expression of NQO 1,HO -1,and Nrf 2,enhancing SOD activity,and increasing GSH content.［Conclusion］ The flavonoid apigenin in D.officinale exerts significant antioxidant effects by activating the Nrf 2 signaling pathway.
Publication Date
7-11-2025
First Page
130
Last Page
136
DOI
10.13652/j.spjx.1003.5788.2025.80308
Recommended Citation
Yixue, WANG; Si, QIN; Runze, ZHOU; and Chenghao, LYU
(2025)
"Antioxidant activity and molecular mechanisms of typical flavonoids in Dendrobium officinale,"
Food and Machinery: Vol. 41:
Iss.
7, Article 18.
DOI: 10.13652/j.spjx.1003.5788.2025.80308
Available at:
https://www.ifoodmm.cn/journal/vol41/iss7/18
References
[1] MA C J,GU C D,LIAN P P,et al.Sulforaphane alleviates psoriasis by enhancing antioxidant defense through KEAP 1-NRF 2 Pathway activation and attenuating inflammatory signaling [J].Cell Death & Disease,2023,14(11):768.
[2] ZHANG P J,LI T,WU X Y,et al.Oxidative stress and diabetes:antioxidative strategies [J].Frontiers of Medicine,2020,14(5):583-600.
[3] CHEN Q M.Nrf 2 for cardiac protection:pharmacological options against oxidative stress [J].Trends in Pharmacological Sciences,2021,42(9):729-744.
[4] GUAN T Z,LI N,XU X X,et al.Involvement of the Keap 1-Nrf2-ARE pathway in the antioxidant activity of sinomenine [J].Archives of Biochemistry and Biophysics,2024,753:109928.字母不同表示差异显著 （P＜0.05）
[5] SHIRAIWA M,KITAKAZE T,YAMASHITA Y,et al.Pectolinarigenin induces antioxidant enzymes through Nrf 2/ARE pathway in HepG 2 cells [J].Antioxidants,2022,11(4):675.
[6] LUO Z F,LIU L,NIE Q,et al.HPLC-based metabolomics of Dendrobium officinale revealing its antioxidant ability [J].Frontiers in Plant Science,2023,14:1060242.
[7] 孙凤婷,王旭,韩新雨,等.复硝酚钠对铁皮石斛中黄酮含量和抗氧化活性的影响 [J].浙江农业学报,2025,37(4):934-942.SUN F T,WANG X,HAN X Y,et al.Effect of sodium nitrophenate on flavonoid content and antioxidant activity in Dendrobium officinale [J].Acta Agriculturae Zhejiangensis,2025,37(4):934-942.
[8] WU F Q,ZHANG Y,LIU W J,et al.Comparison of torrefied and lyophilized Dendrobii Officinalis Caulis (Tiepishihu ) by Fourier transform infrared spectroscopy and two-dimensional correlation spectroscopy [J].Journal of Molecular Structure,2020,1 204:127554.
[9] 农彦彦,吴子瑜,林诗堃,等.高效液相色谱法测定铁皮石斛花中总黄酮的含量 [J].顺德职业技术学院学报,2020,18(1):8-11.NONG Y Y,WU Z Y,LIN S K,et al.Determination of total flavones in dendrobium officinale flower by HPLC [J].Journal of Shunde Polytechnic,2020,18(1):8-11.
[10] XU X Y,ZHANG C,WANG N,et al.Bioactivities and mechanism of actions of Dendrobium officinale:a comprehensive review [J].Oxidative Medicine and Cellular Longevity,2022,2 022:6293355.
[11] 罗继娜,许颖,范钦,等.铁皮石斛粗多糖调控 NF-κB信号通路抗肝纤维化作用及其超声评价 [J/OL ].中药药理与临床.(2025 -03-04) [2025 -04-12].https://doi.org/10.13412 /j.cnki.zyyl.20250303.007.LUO J N,XU Y,FAN Q,et al.Effect of crude polysaccharide from Dendrobium officinalis on NF- κB signaling pathway and its ultrasonic evaluation on liver fibrosis [J/OL ].Pharmacology and Clinic of Chinese Medicine.(2025 -03-04) [2025 -04-12].https://doi.org/10.13412 /j.cnki.zyyl.20250303.007.
[12] 陈漪汶,文霞,黄健聪,等.铁皮石斛的抗氧化、保湿功效研究和应用现状 [J].广州化工,2022,50(13):27-31.CHEN Y W,WEN X,HUANG J C,et al.Research progress on antioxidant and moisturizing effect of dendrobium candidum [J].Guangzhou Chemical Industry,2022,50(13):27-31.
[13] 梁芷韵,谢镇山,黄月纯,等.铁皮石斛黄酮苷类成分 HPLC特征图谱优化及不同种源特征性分析 [J].中国实验方剂学杂志,2019,25(1):22-28.LIANG Z Y,XIE Z S,HUANG Y C,et al.HPLC characteristic spectrum optimization of flavonoid glycosides on Dendrobium officinale and characteristics analysis of different provenances [J].Chinese Journal of Experimental Traditional Medical Formulae,2019,25(1):22-28.
[14] 孙凤婷,许振岚,朱作艺,等.铁皮石斛的黄酮类成分测定及其生物可及性研究 [J].浙江农业学报,2023,35(11):2 710-2 719.SUN F T,XU Z L,ZHU Z Y,et al.Determination of flavonoids in Dendrobium officinale kimura et migo and study on its bioavailability [J].Acta Agriculturae Zhejiangensis,2023,35(11):2 710-2 719.
[15] SI C,ZENG D Q,YU Z M,et al.Transcriptomic and metabolomic analyses reveal the main metabolites in Dendrobium officinale leaves during the harvesting period [J].Plant Physiology and Biochemistry,2022,190:24-34.
[16] SONG J Y,WANG H R,SHENG J Y,et al.Vitexin attenuates chronic kidney disease by inhibiting renal tubular epithelial cell ferroptosis via NRF 2 activation [J].Molecular Medicine,2023,29(1):147.
[17] 罗颖懿,李运容,雷胄熙,等.铁皮石斛共性黄酮类成分新西兰牡荆苷 Ⅱ的体外抗氧化与诱导 HepG 2细胞凋亡的作用 [J].中国实验方剂学杂志,2019,25(1):43-50.LUO Y Y,LI Y R,LEI Z X,et al.Antioxidation activities in vitro of vicenin Ⅱ isolated from Dendrobii officinalis caulis and effect on HepG 2 cells [J].Chinese Journal of Experimental Traditional Medical Formulae,2019,25(1):43-50.
[18] ZHANG B X,WANG J,ZHAO G D,et al.Apigenin protects human melanocytes against oxidative damage by activation of the Nrf 2 pathway [J].Cell Stress and Chaperones,2020,25(2):277-285.
[19] KASHYAP P,SHIKHA D,THAKUR M,et al.Functionality of apigenin as a potent antioxidant with emphasis on bioavailability,metabolism,action mechanism and in vitro and in vivo studies:a review [J].Journal of Food Biochemistry,2022,46(4):e13950.
[20] YAO H,HE Q M,HUANG C,et al.Panaxatriol saponin ameliorates myocardial infarction-induced cardiac fibrosis by targeting Keap 1/Nrf2 to regulate oxidative stress and inhibit cardiac-fibroblast activation and proliferation [J].Free Radical Biology and Medicine,2022,190:264-275.
[21] 刘新粮.芹菜素介导 AMPK/Nrf 2/HO- 1信号通路在心肌缺血/再灌注损伤中的保护作用及机制研究 [D].南昌:南昌大学,2022:36-58.LIU X L.The protective effect and mechanism of apigenin in myocardial ischemia-reperfusion injury by regulating AMPK/Nrf2/HO- 1 signaling pathway [D].Nanchang:Nanchang University,2022:36-58.
[22] TIE F F,DING J,HU N,et al.Kaempferol and kaempferide attenuate oleic acid-induced lipid accumulation and oxidative stress in HepG 2 cells [J].International Journal of Molecular Sciences,2021,22(16):8 847.
[23] 周泓妍,郑怡,王海东,等.南、北五味子蛋白抗氧化活性和对HepG 2细胞氧化应激损伤的修复作用 [J].食品与发酵工业,2024,50(7):51-60.ZHOU H Y,ZHENG Y,WANG H D,et al.Antioxidant activity of Schisandra sphenanthera and Schisandra chinensis protein on HepG 2 cells [J].Food and Fermentation Industries,2024,50(7):51-60.