Abstract
［Objective］ To clarify the main components of Lycium barbarum polysaccharides that exert an anti -fatigue effect.［Methods］ Lycium barbarum polysaccharides with different relative molecular weights are prepared by water extraction,ultrafiltration membrane fractionation dialysis,and other methods.The components with good anti -fatigue activity are obtained by mice burden swimming test.They are then eluted and purified by ion exchange column and gel chromatography column to determine the relative molecular weights and monosaccharide components.The infrared spectrum is employed to analyze.［Results］］ The content of polysaccharide,protein,and uronic acid in Lycium barbarum polysaccharides with different molecular weights is 63.62%~75.63%,1.77%~2.63%,and 17.77%~24.77%,respectively.The LBP 2 has a higher component yield.The relative molecular weight of the four components is 115.3×103,47.4×103,8.14×103,and 4.82×103,respectively.The LBP 2 is superior to other components in prolonging swimming time,reducing urea nitrogen and blood lactate content,increasing muscle glycogen and liver glycogen content,and enhancing the activity of superoxide dismutase,peroxidase,and glutathione peroxidase (P<0.05);LBP 2 is purified to obtain acidic polysaccharides LBPE 1,LBPE 2,and LBPE 3.The relative molecular weight of LBPE 1 and LBPE 2 is 3.54×104 and 1.10×104,respectively.The main components are mannose,rhamnose,galacturonic acid,glucose,galactose,and arabinose,with molar ratios of 0.73∶1.61∶0.53∶3.22∶6.33∶8.26 and 1.94∶1.81∶1.36∶6.77∶8.46∶6.57,respectively.LBPE 1 and LBPE 2 have multiple distinct polysaccharide characteristic absorption peaks,both of which are β-pyranoside polysaccharides.［Conclusion］ The polysaccharides LBPE 1 and LBPE 2 from Lycium barbarum polysaccharide are the main structural components that exert an anti -fatigue effect.引用格式：孙鑫，范士君.枸杞多糖的抗疲劳活性研究 ［J］.食品与机械，2025，41（7）：175-182.C itation:SUN Xin,FAN Shijun.Anti-fatigue activity of Lycium barbarum polysaccharides [J].Food & Machinery,2025,41(7):175-182.
Publication Date
7-11-2025
First Page
175
Last Page
182
DOI
10.13652/j.spjx.1003.5788.2024.81082
Recommended Citation
Xin, SUN and Shijun, FAN
(2025)
"Anti-fatigue activity of Lycium barbarum polysaccharides,"
Food and Machinery: Vol. 41:
Iss.
7, Article 23.
DOI: 10.13652/j.spjx.1003.5788.2024.81082
Available at:
https://www.ifoodmm.cn/journal/vol41/iss7/23
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