Abstract
Veterinary drugs play a crucial role in ensuring the healthy development of the livestock industry.However,improper or excessive use of veterinary drugs may pose threats to the environment and human health.To achieve effective monitoring of veterinary drug residues,various detection technologies have emerged.Among them,molecularly imprinted electrochemical sensors (MIECSs ) combines the high recognition specificity of molecularly imprinted polymers (MIPs ) with the rapid and sensitive advantages of electrochemical analysis,becoming a powerful tools in the detection of veterinary drug residues and demonstrating great application potential.However,its transition to large -scale application still faces many challenges.This article systematically reviews the progress in the application of MIECS in the detection of different types of veterinary drug residues (antibiotics,sulfonamides,hormones,sedatives,antiparasitics and nitrofurans ) and elaborates on its future research directions and application prospects.
Publication Date
7-11-2025
First Page
207
Last Page
218
DOI
10.13652/j.spjx.1003.5788.2025.80583
Recommended Citation
Yanrong, HAN; Yanrong, CHEN; Chenyu, WANG; Yishan, KUANG; and Bo, ZHANG
(2025)
"Progress in the application of molecularly imprinted electrochemical sensors in the detection of veterinary drug residues,"
Food and Machinery: Vol. 41:
Iss.
7, Article 27.
DOI: 10.13652/j.spjx.1003.5788.2025.80583
Available at:
https://www.ifoodmm.cn/journal/vol41/iss7/27
References
[1] 周迎春,刘少博,华向美.我国动物源性食品中兽药残留的危害及现状 [J].粮食与油脂,2021,34(6):18-20.ZHOU Y C,LIU S B,HUA X M.The hazard and status quo of veterinary drug residues in animal derived food in China [J].Cereals & Oils,2021,34(6):18-20.
[2] YANG Y F,ZHANG H,ZHOU G Y,et al.Risk assessment of veterinary drug residues in pork on the market in the people's republic of China [J].Journal of Food Protection,2022,85(5):815-827.
[3] SIN J E V,SHEN P,TEO G S,et al.Surveillance of veterinary drug residues in food commonly consumed in Singapore and assessment of dietary exposure [J].Heliyon,2023,9(11):e21160.
[4] CHARUAUD L,JARDÉ E,JAFFRÉZIC A,et al.Veterinary pharmaceutical residues in water resources and tap water in an intensive husbandry area in France [J].Science of the Total Environment,2019,664:605-615.
[5] CHARUAUD L,JARDÉ E,JAFFRÉZIC A,et al.Veterinary pharmaceutical residues from natural water to tap water:sales,occurrence and fate [J].Journal of Hazardous Materials,2019,361:169-186.
[6] KIM Y R,PARK D,SIM J H,et al.National long-term monitoring and risk assessment of various veterinary drug residues in livestock products in the Republic of Korea [J].Food Control,2025,167:110818.
[7] 李宏,向俊,李丹,等.QuEChERS-UPLC-MS/MS 同时测定鸡肉中 80种兽药残留 [J].食品与机械,2023,39(6):48-54,80.LI H,XIANG J,LI D,et al.Simultaneous determination of 80 veterinary drug residues in chicken by QuEChERS-UPLC-MS/MS[J].Food & Machinery,2023,39(6):48-54,80.
[8] SOARES V M,PEREIRA J G,BARRETO F,et al.Residues of veterinary drugs in animal products commercialized in the border region of Brazil,Argentina,and Uruguay [J].Journal of Food Protection,2022,85(6):980-986.
[9] OZBAY G,BABU B K,PEATMAN E,et al.Prescreening veterinary drug residues,heavy metal concentration,and genetic authentication in retail catfish fillets in the Northeast United States [J].Food Control,2022,135:108792.
[10] BRANDI J,SIRAGUSA G,ROBOTTI E,et al.Analysis of veterinary drugs and pesticides in food using liquid chromatography-mass spectrometry [J].TrAC Trends in Analytical Chemistry,2024,179:117888.
[11] JADHAV M R,PUDALE A,RAUT P,et al.A unified approach for high-throughput quantitative analysis of the residues of multi-class veterinary drugs and pesticides in bovine milk using LC-MS/MS and GC-MS/MS [J].Food Chemistry,2019,272:292-305.
[12] ZHOU Y S,WANG J N,WU B L,et al.Rapid determination of furazolidone residues in animal foods by time-resolved fluorescence immunochromatography [J].Food Chemistry,2024,445:138711.
[13] SHENG W,LI Y Z,XU X,et al.Enzyme-linked immunosorbent assay and colloidal gold-based immunochromatographic assay for several (fluoro ) quinolones in milk [J].Microchimica Acta,2011,173:307-316.
[14] CETINKAYA A,KAYA S I,OZKAN S A.A review of point-of-care (POC ) and lab-on-chip (LOC ) approaches in molecularly imprinted polymer-based electrochemical sensors for biomedical applications [J].Analytica Chimica Acta,2025:344080.
[15] HE J B,WANG L,LIU H L,et al.Recent advances in molecularly imprinted polymers (MIPs ) for visual recognition and inhibition of α-dicarbonyl compound-mediated Maillard reaction products [J].Food Chemistry,2024,446:138 839-138 851.
[16] LI Y X,LUO L X,KONG Y Q,et al.Recent advances in molecularly imprinted polymer-based electrochemical sensors[J].Biosensors & Bioelectronics,2024,249:116018.
[17] WANG L,PAGETT M,ZHANG W.Molecularly imprinted polymer (MIP ) based electrochemical sensors and their recent advances in health applications [J].Sensors and Actuators |Reports,2023,5:100153.
[18] GENG L J,SUN J S,LIU M Y,et al.Molecularly imprinted polymers-aptamer electrochemical sensor based on dual recognition strategy for high sensitivity detection of chloramphenicol [J].Food Chemistry,2024,437:137933.
[19] ZHOU Y Q,ABDUREXIT A,JAMAL R,et al.Highly sensitive electrochemical sensing of norfloxacin by molecularly imprinted composite hollow spheres [J].Biosensors & Bioelectronics,2024,251:116119.
[20] XIONG Y Q,GAO X Y,WANG Y,et al.A combination of polypeptide and magnetic molecular imprinted electrochemical sensor based on BSA-AuNPs/Fe3O4@ ZIF- 8 for the detection of norfloxacin [J].Microchemical Journal,2024,201:110583.
[21] FU Y C,XIE Y,SHI H Z,et al.Molecularly imprinted electrochemical sensor based on metal-covalent organic framework for specifically recognizing norfloxacin from unpretreated milk [J].Food Chemistry,2023,429:136921.
[22] XIONG Y Q,ZHANG D,YE C Z,et al.Ultra-sensitive detection of ciprofloxacin hydrochloride in milk by molecularly imprinted electrochemical sensor based on S-CoFe-MOFs/AuNPs [J].Journal of Food Composition and Analysis,2023,122:105439.
[23] JIN S W,ZHANG Y J,LIU R L,et al.A novel molecularly imprinted electrochemical sensor based on MnO-Fe3O4@ C was designed with DFT theoretical study for the detection of thiamphenicol in food [J].Colloids and Surfaces A:Physicochemical and Engineering Aspects,2025,705:135572.
[24] SUN R N,HAN S,ZONG W,et al.Ultrasensitive detection of chlortetracycline in animal-origin food using molecularly imprinted electrochemical sensor based on SnS2/ZnCo-MOF and AuNPs [J].Food Chemistry,2024,452:139537.
[25] ZHANG M S,ZHANG B J,LI T B,et al.Electrochemical detection of aminoglycoside antibiotics residuals in milk based on magnetic molecularly imprinted particles and metal ions [J].Food Chemistry,2022,389:133120.
[26] CHENG Q,ABDIRYIM T,JAMAL R,et al.A novel molecularly imprinted electrochemical sensor from poly (3,4-ethylenedioxythiophene )/chitosan for selective and sensitive detection of levofloxacin [J].International Journal of Biological Macromolecules,2024,267:131321.
[27] KOÇAK İ.ZnO and Au nanoparticles supported highly sensitive and selective electrochemical sensor based on molecularly imprinted polymer for sulfaguanidine and sulfamerazine detection [J].Journal of Pharmaceutical and Biomedical Analysis,2023,234:115518.
[28] SUN Y F,HE J B,WATERHOUSE G I N,et al.A selective molecularly imprinted electrochemical sensor with GO@ COF signal amplification for the simultaneous determination of sulfadiazine and acetaminophen [J].Sensors and Actuators B:Chemical,2019,300:126993.
[29] YU X F,YANG Y,SHEN Q R,et al.A novel differential ratiometric molecularly imprinted electrochemical sensor for determination of sulfadiazine in food samples [J].Food Chemistry,2024,434:137461.
[30] NIU Z J,SHI Y K,LIU S J,et al.DFT-assisted design of a electrochemical sensor based on MIP/CNT/MoS2-CoNi for the detection of sulfamethazine in meat [J].Journal of Food Composition and Analysis,2025,140:107261.
[31] HATAMLUYI B,SADEGHZADEH S,BALOUCH ZEHI Z,et al.A rapid and recyclable analysis method for sulfadimethoxine detection based on molecularly imprinted electrochemical sensor reinforced by GQDs/ZIF- 8 nanocomposite [J].Microchemical Journal,2023,185:108266.
[32] YANG H X,HU M N,YAN H X,et al.A novel composite of few-layer Nb2CTx and molecularly imprinted polymer with alkenyl ferrocene as cross-linker for ratiometric electrochemical detection of sulfamethoxazole [J].Microchemical Journal,2025,208:112540.
[33] HAN B,LI W B,SHEN Y,et al.Improving the sensitivity and selectivity of sulfonamides electrochemical detection with double-system imprinted polymers [J].Science of The Total Environment,2023,864:161173.
[34] BAI S M,YANG T T,LIU P Q,et al.Preparation of a V-COF@ SWCNTs-COOH/SPCE supported molecularly imprinted electrochemical sensor for real-time detection of trace sulfadimidine [J].Talanta,2025,282:127046.
[35] MURUGESAN K,KUMAR M D,KANIRAJA G,et al.Theoretical screening and electrochemical sensor for determination of norepinephrine using a molecularly imprinted poly (3-amiophenylboronic acid ) [J].Analytical Biochemistry,2025,696:115676.
[36] WANG J S,SUN L Y,ZUO Y X,et al.A highly sensitive molecularly-imprinted electrochemical sensor based on a conducting PEDOT/SA hydrogel for the detection of cortisol with exceptional antifouling properties [J].Sensors and Actuators B:Chemical,2025,426:137133.
[37] MANI A,ANIRUDHAN T S.Electrochemical sensing of cortisol by gold nanoparticle incorporated carboxylated graphene oxide based molecularly imprinted polymer [J].Chemical Engineering Journal,2024,493:152654.
[38] DONG J M,LI J Y,XU L D,et al.A molecularly imprinted electrochemical sensor based on Au nanoparticles-poly (o-phenylenediamine ) for diethylstilbestrol detection [J].Synthetic Metals,2025,311:117814.
[39] SUN X Y,LIU M M,LIU H,et al.A molecularly imprinted electrochemical aptasensor-based dual recognition elements for selective detection of dexamethasone [J].Talanta,2024,277:126404.
[40] FLOREA A,CRISTEA C,VOCANSON F,et al.Electrochemical sensor for the detection of estradiol based on electropolymerized molecularly imprinted polythioaniline film with signal amplification using gold nanoparticles [J].Electrochemistry Communications,2015,59:36-39.
[41] SANCHEZ-ALMIROLA J,GAGE A,LOPEZ R,et al.Label and bio-active free electrochemical detection of testosterone hormone using MIP-based sensing platform [J].Materials Science and Engineering:B,2023,296:116670.
[42] HUANG Y,YE D X,YANG J,et al.Dual recognition elements for selective determination of progesterone based on molecularly imprinted electrochemical aptasensor [J].Analytica Chimica Acta,2023,1 264:341288.
[43] MOTAHARIAN A,HOSSEINI M R M,NASERI K.Determination of psychotropic drug chlorpromazine using screen printed carbon electrodes modified with novel MIP-MWCNTs nano-composite prepared by suspension polymerization method [J].Sensors and Actuators B:Chemical,2019,288:356-362.
[44] YUAN J X,WANG S L,CHEN L,et al.A novel ratiometric electrochemical sensor based on bifunctional copper-coordinated polydopamine molecular imprinting for the detection of chlorpromazine [J].Sensors and Actuators B:Chemical,2025,440:137910.
[45] LU Z W,LI Y F,LIU T,et al.A dual-template imprinted polymer electrochemical sensor based on AuNPs and nitrogen-doped graphene oxide quantum dots coated on NiS2/biomass carbon for simultaneous determination of dopamine and chlorpromazine [J].Chemical Engineering Journal,2020,389:124417.
[46] LIU Y W,HU X P,XIA Y D,et al.A novel ratiometric electrochemical sensor based on dual-monomer molecularly imprinted polymer and Pt/Co3O4 for sensitive detection of chlorpromazine hydrochloride [J].Analytica Chimica Acta,2022,1 190:339245.
[47] LU Z W,WEI K,MA H,et al.Nanoarchitectonics of on-off ratiometric signal amplified electrochemical sensor for chlorpromazine with molecularly imprinted polymer based on Ni-MOF/Fe-MOF- 5 hybrid Au nanoparticles [J].Separation and Purification Technology,2023,327:124858.
[48] SOLIMAN S S,MAHMOUD A M,ELGHOBASHY M R,et al.Point-of-care electrochemical sensor for selective determination of date rape drug "ketamine" based on core-shell molecularly imprinted polymer [J].Talanta,2023,254:124151.
[49] ALIZADEH T,AKHOUNDIAN M.Promethazine determination in plasma samples by using carbon paste electrode modified with molecularly imprinted polymer (MIP ):Coupling of extraction,preconcentration and electrochemical determination [J].Electrochimica Acta,2010,55(20):5 867-5 873.
[50] ZHENG Q,CAO Y Y,CHEN Y,et al.Portable electrochemical test strip based on Au/graphene for rapid on-site detection of xylazine in raw milk [J].Microchemical Journal,2025,212:113205.
[51] FU K X,ZHANG R L,HE J C,et al.Sensitive detection of ketamine with an electrochemical sensor based on UV-induced polymerized molecularly imprinted membranes at graphene and MOFs modified electrode [J].Biosensors & Bioelectronics,2019,143:111636.
[52] PANAHI Y,MOTAHARIAN A,HOSSEINI M R M,et al.High sensitive and selective nano-molecularly imprinted polymer based electrochemical sensor for midazolam drug detection in pharmaceutical formulation and human urine samples [J].Sensors and Actuators B:Chemical,2018,273:1 579-1 586.
[53] LI S H,WU Y W,MA X H,et al.Monitoring levamisole in food and the environment with high selectivity using an electrochemical chiral sensor comprising an MOF and molecularly imprinted polymer [J].Food Chemistry,2024,430:137105.
[54] RADI A E,EL-NAGGAR A E,NASSEF H M.Molecularly imprinted polymer based electrochemical sensor for the determination of the anthelmintic drug oxfendazole [J].Journal of Electroanalytical Chemistry,2014,729:135-141.
[55] CHENG J,LI Y F,ZHONG J,et al.Molecularly imprinted electrochemical sensor based on biomass carbon decorated with MOF-derived Cr2O3 and silver nanoparticles for selective and sensitive detection of nitrofurazone [J].Chemical Engineering Journal,2020,398:125664.
[56] LU H,LIU M M,CUI H Y,et al.An advanced molecularly imprinted electrochemical sensor based bifunctional monomers for highly sensitive detection of nitrofurazone [J].Electrochimica Acta,2022,427:140858.
[57] LIU T,HE J,LU Z W,et al.A visual electrochemiluminescence molecularly imprinted sensor with Ag+@UiO- 66-NH2 decorated CsPbBr3 perovskite based on smartphone for point-of-care detection of nitrofurazone [J].Chemical Engineering Journal,2022,429:132462.
[58] ZHANG X,LIU R,LIU R J,et al.Target detection and simultaneous degradation of furazolidone by molecularly imprinted CoWO4/g-C3N4[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects,2025,705:135694.