Abstract
［Objective］ To establish a method for determining the total vitamin B12 content in infant formula milk powder based on the indirect detection of α-ribazole,a degradation product and nucleoside component of vitamin B12.［Methods］ After protein denaturation and sugar removal,the sample underwent acid hydrolysis and enzymatic dephosphorylation to release α-ribazole,which was then purified using a boronate affinity column.Quantitative analysis was performed using hydrophilic interaction liquid chromatography with fluorescence detection (HILIC -FLD ).［Results］］ Alpha -ribazole showed good linearity in the range of 1.00~10.0 μg/L,with a correlation coefficient (R²) of 0.999 1.The spiked recovery ranged from 72.5% to 78.3%,with relative standard deviations (RSDs ) between 4.56% and 7.28%.The limit of detection (LOD ) and limit of quantification (LOQ ) were 0.4 μg/100 g and 1.2 μg/100 g,respectively.Bland -Altman analysis showed no significant difference between this method and the national standard method,and the consistency was good.［Conclusion］ This method is highly sensitive and reproducible,and it can accurately determine the total vitamin B12 content in infant formula milk powder without the need for cyanide pretreatment.
Publication Date
7-11-2025
First Page
39
Last Page
45
DOI
10.13652/j.spjx.1003.5788.2025.80464
Recommended Citation
Liang, LI; Wenjuan, DENG; Weiqian, HUANG; Junfa, WU; and Tianfu, QIN
(2025)
"Determination of vitamin B12 in infant formula milk powder by cyanide-free degradation coupled with hydrophilic interaction liquid chromatography with fluorescence detection,"
Food and Machinery: Vol. 41:
Iss.
7, Article 6.
DOI: 10.13652/j.spjx.1003.5788.2025.80464
Available at:
https://www.ifoodmm.cn/journal/vol41/iss7/6
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