Abstract
[Objective] To analyze the effects of Procambarus clarkii shell bioactive peptides (PCSBP) on the growth performance, muscle antioxidant capacity, and circadian rhythm related gene expression of Pelodiscus sinensis. [Methods] In this experiment, different concentrations (0.05%, 0.10%, 0.20%, and 0.50%) of PCSBP are added to the diets of P.sinensis for 70 days. The growth performance, muscle antioxidant capacity, and expression levels of circadian rhythm related genes are measured to preliminarily explore the regulatory effect of PCSBP on the muscle antioxidant capacity and circadian rhythm of P.sinensis. [Results] With the increase of PCSBP concentration in diets, the final body weight, weight gain rate, and specific growth rate of P.sinensis show a significant upward trend, and 引用格式：杨赛杰，李虹辉，黄超，等. 虾壳活性肽对中华鳖肌肉抗氧化能力和昼夜节律的调控作用 [J]. 食品与机械，2025，41(8):126-134. Citation: YANG Saijie, LI Honghui, HUANG Chao, et al. Regulatory effect of Procambarus clarkii shell bioactive peptides on muscle antioxidant capacity and circadian rhythm of Pelodiscus sinensis [J]. Food & Machinery, 2025, 41(8):126-134. the feed conversion rate decreases. The activities of total superoxide dismutase (T-SOD), catalase (CAT), and glutathione peroxidase (GSH-PX) in the muscle of P.sinensis show a significant increase trend with the increase of PCSBP concentration. Meanwhile, PCSBP significantly up-regulates the expression of nrf2 and keap 1, key signaling molecules for antioxidant capacity and activates the expression of antioxidant enzyme genes sod1, sod2, cat, gpx1, gpx2, gpx4, gstk1, and gstz1. In addition, PCSBP significantly regulates the expression of circadian rhythm core genes bmal 1, clock, nr1d2, per1, per2, cry1, cry2, and npas 2. There is a significant correlation between the antioxidant signaling pathway of nrf2-keap 1 mediated by PCSBP and the expression of core genes of circadian rhythm. It is significantly positively correlated with the expression of bmal 1, clock, per2, cry1, cry2, and aanat, while significantly negatively correlated with the expression of npas 2, rora, and rorc. [Conclusion] PCSBP has a regulatory effect on the antioxidant capacity and circadian rhythm of P.sinensis, effectively enhancing the body's oxidative stress resistance.
Publication Date
9-25-2025
First Page
126
Last Page
134
DOI
10.13652/j.spjx.1003.5788.2025.80303
Recommended Citation
Saijie, YANG; Honghui, LI; Chao, HUANG; Hucheng, YANG; Yanli, LEI; and Jie, ZHOU
(2025)
"Regulatory effect of Procambarus clarkii shell bioactive peptides on muscle antioxidant capacity and circadian rhythm of Pelodiscus sinensis,"
Food and Machinery: Vol. 41:
Iss.
8, Article 17.
DOI: 10.13652/j.spjx.1003.5788.2025.80303
Available at:
https://www.ifoodmm.cn/journal/vol41/iss8/17
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