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Corresponding Author(s)

黄鹭强（1974—），男，福建师范大学教授，博士。E-mail：biohlq@fjnu.edu.cn

Abstract

[Objective] To study the anti-aging effects of kelp (Laminaria japonica) polysaccharides. [Methods] Polysaccharide is obtained from kelp by acid-assisted extraction methods. Based on the aging model of Caenorhabditis elegans, the animal's lipofuscin, reactive oxygen species (ROS), antioxidant capacity, and aging-related gene expression are analyzed to evaluate the effects of kelp polysaccharides on their life span, motility, and stress resistance. [Results] Compared with the control group, acid-extracted kelp polysaccharides (AKP) with different mass concentrations improve the life span, mobility, and stress resistance of C.elegans. Specifically, AKP significantly reduces the lipofuscin content and ROS levels, while increasing the activities of catalase and superoxide dismutase. In addition, kelp polysaccharides extend the life span of C.elegans by activating the expression of skn-1, daf- 2, age- 1, akt- 1, and daf-16 mRNA, while up-regulating the expression of downstream factor mRNAs. [Conclusion] AKP can extend life span by increasing antioxidant capacity and regulating the insulin/insulin-like growth factor signaling pathway (IIS) of C.elegans.

Publication Date

9-25-2025

First Page

135

Last Page

142

DOI

10.13652/j.spjx.1003.5788.2024.81184

References

[1] AGHAJANPOUR M,NAZER M R,OBEIDAVI Z,et al.Functional foods and their role in cancer prevention and health promotion:a comprehensive review [J].American Journal of Cancer Research,2017,7(4):740-769.
[2] RAHMAN A,REHMANI R,PIRVU D G,et al.Unlocking the therapeutic potential of marine collagen:a scientific exploration for delaying skin aging [J].Marine Drugs,2024,22(4):159.
[3] ZHAO X,LI B F,XUE C H,et al.Effect of molecular weight on the antioxidant property of low molecular weight alginate from Laminaria japonica [J].Journal of Applied Phycology,2012,24(2):295-300.
[4] LAUKAITYTE S,RIERA R.The status of research and utilisation on the subtidal kelp along the Chilean coast:a literature review [J].Acta Oceanologica Sinica,2022,41(12):7-17.
[5] LI H Y,YI Y L,GUO S,et al.Isolation,structural characterization and bioactivities of polysaccharides from Laminaria japonica:a review [J].Food Chem,2022,370:131010.
[6] CHEN H,WU Y,CHEN Y,et al.P-268 Seaweed Laminaria japonica peptides possess strong anti-liver cancer effects [J].Annals of Oncology,2021,32:S189.
[7] OZAWA T,YAMAMOTO J,YAMAGISHI T,et al.Two fucoidans in the holdfast of cultivated Laminaria japonica [J].Journal of Natural Medicines,2006,60(3):236-239.
[8] GUAN X,WANG F Y,ZHOU B Q,et al.The nutritional function of active polysaccharides from marine animals:a review [J].Food Bioscience,2024,58:103693.
[9] 葛智超,李燕,施文正,等.裸藻多糖提取工艺优化及抗氧化活性研究 [J].食品与机械,2020,36(1):186-191.GE Z C,LI Y,SHI W Z,et al.Optimization of the extraction process of polysaccharides from euglena and its antioxidant activity [J].Food & Machinery,2020,36(1):186-191.
[10] HOU Y,WANG J,JIN W H,et al.Degradation of Laminaria japonica fucoidan by hydrogen peroxide and antioxidant activities of the degradation products of different molecular weights [J].Carbohydrate Polymers,2012,87(1):153-159.
[11] 王猛,关思宇,于杰,等.褐藻多糖的体外抗氧化活性及其延长秀丽隐杆线虫的寿命 [J].现代食品科技,2022,38(4):1-9.WANG M,GUAN S Y,YU J,et al.In vitro antioxidant activity of fucoidan-containing sulfated polysaccharide and its prolongation of the lifespan of Caenorhabditis elegans [J].Modern Food Science and Technology,2022,38(4):1-9.
[12] POUPET C,CHASSARD C,NIVOLIEZ A,et al.Caenorhabditis elegans,a host to investigate the probiotic properties of beneficial microorganisms [J].Frontiers in Nutrition,2020,7:135.
[13] 陈旺,何海伦,郑艳君,等.秀丽隐杆线虫在食品营养功能评价中的研究进展 [J].食品与机械,2024,40(1):197-203.CHEN W,HE H L,ZHENG Y J,et al.Review of Caenorhabditis elegans in food nutritional function evaluation[J].Food & Machinery,2024,40(1):197-203.
[14] LIN C,ZHANG X,XIAO J,et al.Effects on longevity extension and mechanism of action of carnosic acid in Caenorhabditis elegans [J].Food & Funct,2019,10(3):1 398-1 410.
[15] MCINTYRE G,WRIGHT J,WONG H T,et al.Effects of FUdR on gene expression in the C.elegans bacterial diet OP 50[J].BMC Research Notes,2021,14(1):207.
[16] WANG H,ZHAO J,YANG S N,et al.Anti-aging effects and underling mechanism of D-chiro-inosiol on glucose-induced oxidative damage in Caenorhabditis elegans [J].Science and Technology of Food Industry,2019,40(2):282-286.
[17] SONG B B,XIA W,LI T,et al.Mitochondria are involved in the combination of blueberry and apple peel extracts synergistically ameliorating the lifespan and oxidative stress in Caenorhabditis elegans [J].Food & Function,2022,13(15):8 204-8 213.
[18] CHANDLER-BROWN D,CHOI H,PARK S,et al.Sorbitol treatment extends lifespan and induces the osmotic stress response in Caenorhabditis elegans [J].Frontiers in Genetics,2015,6:316.
[19] BRESOLÍ -OBACH R,BUSTO-MONER L,MULLER C,et al.NanoDCFH-DA:a silica-based nanostructured fluorogenic probe for the detection of reactive oxygen species [J].Photochemistry and Photobiology,2018,94(6):1 143-1 150.
[20] LI Z H,CUI B,LIU X W,et al.Virucidal activity and the antiviral mechanism of acidic polysaccharides against Enterovirus 71 infection in vitro [J].Microbiology and Immunology,2020,64(3):189-201.
[21] BILAN M I,KLOCHKOVA N G,SHASHKOV A S,et al.Polysaccharides of Algae 71*.Polysaccharides of the Pacific brown alga Alaria marginata [J].Russian Chemical Bulletin,2018,67(1):137-143.
[22] JAYAPALA N,TORAGALL V,GNANESH KUMAR B S,et al.Preparation,characterization,radical scavenging property and antidiabetic potential of laminaria oligosaccharides derived from laminarin [J].Algal Research,2022,63:102642.
[23] LESANPEZESHKI L,QADOTA H,DARABAD M N,et al.Investigating the correlation of muscle function tests and sarcomere organization in C.elegans [J].Skeletal Muscle,2021,11(1):20.
[24] JIN S Y,LI D Q,LU S,et al.Ethanol extracts of Panax notoginseng increase lifespan and protect against oxidative stress in Caenorhabditis elegans via the insulin/IGF- 1 signaling pathway [J].Journal of Functional Foods,2019,58:218-226.
[25] PAPAEVGENIOU N,HOEHN A,TUR J A,et al.Sugar-derived AGEs accelerate pharyngeal pumping rate and increase the lifespan of Caenorhabditis elegans [J].Free Radical Research,2019,53(sup1):1 056-1 067.
[26] 施滢,王平,代君怡,等.基于秀丽隐杆线虫模型探讨白芥子水提物抗氧化作用及机制 [J].食品安全质量检测学报,2022,13(23):7 773-7 778.SHI Y,WANG P,DAI J Y,et al.Study on antioxidative effect and mechanism of water extracts of white mustard seed based on Caenorhabditis elegans model [J].Journal of Food Safety & Quality,2022,13(23):7 773-7 778.
[27] LI R,TAO M F,XU T T,et al.Small berries as health-promoting ingredients:a review on anti-aging effects and mechanisms in Caenorhabditis elegans [J].Food & Function,2022,13(2):478-500.
[28] KUČEROVÁ L,KUBRAK O I,BENGTSSON J M,et al.Slowed aging during reproductive dormancy is reflected in genome-wide transcriptome changes in Drosophila melanogaster [J].BMC Genomics,2016,17:50.
[29] LIU H M,LIU B J,ZHANG S Q,et al.Lentinan protects Caenorhabditis elegans against fluopyram-induced toxicity through DAF- 16 and SKN- 1 pathways [J].Ecotoxicology and Environmental Safety,2023,265:115510.
[30] MA J,WANG R R,CHEN T,et al.Protective effects of baicalin in a Caenorhabditis elegans model of Parkinson's disease [J].Toxicology Research,2021,10(3):409-417.
[31] SCHIEBER M,CHANDEL N S.ROS function in redox signaling and oxidative stress [J].Curr Biol,2014,24(10):R453-R462.
[32] DEHGHAN E,ZHANG Y Q,SAREMI B,et al.Hydralazine induces stress resistance and extends C.elegans lifespan by activating the NRF 2/SKN- 1 signalling pathway [J].Nature Communications,2017,8:2 223.
[33] HAVERMANN S,HUMPF H U,WÄTJEN W.Baicalein modulates stress-resistance and life span in C.elegans via SKN- 1 but not DAF- 16[J].Fitoterapia,2016,113:123-127.
[34] SRIVASTAVA S,SAMMI S R,LAXMAN T S,et al.Silymarin promotes longevity and alleviates Parkinson's associated pathologies in Caenorhabditis elegans [J].Journal of Functional Foods,2017,31:32-43.
[35] HOSHIKAWA H,UNO M,HONJOH S,et al.Octopamine enhances oxidative stress resistance through the fasting-responsive transcription factor DAF- 16/FOXO in C.elegans[J].Genes to Cells,2017,22(2):210-219.
[36] KIMURA K D,TISSENBAUM H A,LIU Y,et al.Daf- 2,an insulin receptor-like gene that regulates longevity and diapause in Caenorhabditis elegans [J].Science,1997,277(5 328):942-946.
[37] MARTINS R,LITHGOW G J,LINK W.Long live FOXO:unraveling the role of FOXO proteins in aging and longevity[J].Aging Cell,2016,15(2):196-207.
[38] LUO J R,ZHANG X Y,LI W,et al.Pentagalloyl glucose enhanced the stress resistance to delay aging process in Caenorhabditis elegans [J].Process Biochemistry,2024,146:462-472.
[39] LITHGOW G J,WHITE T M,MELOV S,et al.Thermotolerance and extended life-span conferred by single-gene mutations and induced by thermal stress [J].Proc Natl Acad Sci,1995,92(16):7 540-7 544.
[40] LEWIS K N,WASON E,EDREY Y H,et al.Regulation of Nrf-2 signaling and longevity in naturally long-lived rodents[J].Proceedings of the National Academy of Sciences of the United States of America,2015,112(12):3 722- 3 727.
[41] LEES H,WALTERS H,COX L S.Animal and human models to understand ageing [J].Maturitas,2016,93:18-27.

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