Abstract
[Objective] To enhance the biosynthesis efficiency of gamma-aminobutyric acid (GABA) via submerged fermentation by Lactiplantibacillus plantarum. [Methods] With the activity of whole-cell glutamate decarboxylase (GAD) as an evaluation indicator, the GAD production conditions are optimized through systematic investigation of both fermentation conditions and culture media, and the fermentation process for GABA production is subsequently established based on these optimized conditions. [Results] The optimal fermentation conditions for GAD production by Lpb.plantarum Y-2 are static culture at 30℃ for 48 h in a modified MRS (MMRS) medium containing 10 g/L peptone, 10 g/L beef extract, 5 g/L yeast extract, 10 g/L sucrose, 1.0 g/L Tween 80, 4.8 g/L CH3COONa, 0.03 g/L MnSO4, and 8 g/L L-monosodium glutamate at pH 6.5. Under these conditions, the activity of whole-cell GAD in per liter of fermentation broth is (83.42±4.37) U, which increases by (88.48±9.87)% compared with that in the MRS medium. The yield of GABA synthesized via fermentation in the MMRS medium reaches (2.27±0.12) g/L, representing a (118.27±11.09)% increase compared with that of fermentation in the MRS medium. [Conclusion] Optimizing the production conditions of GAD to enhance the GABA fermentation process serves as an effective strategy to boost the GABA yield.
Publication Date
9-25-2025
First Page
164
Last Page
171
DOI
10.13652/j.spjx.1003.5788.2025.80220
Recommended Citation
Shengyuan, YANG; Genjun, FAN; and Yiying, LIU
(2025)
"Gamma-aminobutyric acid biosynthesis via fermentation using Lactiplantibacillus plantarum with optimized glutamate decarboxylase expression conditions,"
Food and Machinery: Vol. 41:
Iss.
8, Article 22.
DOI: 10.13652/j.spjx.1003.5788.2025.80220
Available at:
https://www.ifoodmm.cn/journal/vol41/iss8/22
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