Abstract
[Objective] To study the extraction process and in vitro activity of polysaccharide from Musella lasiocarpa. [Methods] Ultrasonic-assisted ionic liquid extraction is adopted. With the extraction rate of polysaccharide as the evaluation index, single factor and response surface methods are used to optimize the extraction process of polysaccharide. Furthermore, the polysaccharide is characterized in terms of the antioxidant activity, monosaccharide composition, and inhibitory activities on pancreatic lipase and α-amylase. [Results] The 引用格式：段秋虹，贾庆超. 超声辅助离子液体法提取地涌金莲多糖及体外活性、单糖成分研究 [J]. 食品与机械，2025，41(8):188-196. Citation: DUAN Qiuhong, JIA Qingchao. Extraction of polysaccharide from Musella lasiocarpa by ultrasonic-assisted ionic liquid method and study on the in vitro activity and monosaccharide composition of the polysaccharide [J]. Food & Machinery, 2025, 41(8):188-196. |extraction process of polysaccharide is optimized as follows:extraction power of 200 W, ionic liquid volume of 1.80 mL, ultrasonic time of 32 min, solid-liquid ratio of 1:36, and ultrasonic temperature of 37℃, under which the extraction rate of polysaccharide is (32.20±0.68) mg/g. HPLC shows that the monosaccharides of the purified polysaccharide include mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and arabinose, with the ratio of 1.26∶6.37∶4. 29∶0.86∶0.72∶22. 35∶0.21∶2.46∶0.18. The scavenging rates against DPPH·, ·OH, ABTS+·, and O-2·and the inhibition rates on pancreatic lipase and α-amylase of polysaccharide increase with the increase in mass concentration. When the mass concentration of polysaccharide is 5. 0 mg/mL, the scavenging rates against DPPH·, ·OH, ABTS+·, and O-2·are 78.60%, 67.70%, 79.85%, and 69.67%, respectively. The IC50 values show that the scavenging abilities of the polysaccharide against free radicals follow the trend of DPPH·>ABTS+·>·OH>O-2·. The polysaccharide at the mass concentration of 10 mg/mL shows the inhibition rates of 67.2% and 58.3% on pancreatic lipase and α-amylase, respectively. [Conclusion] The ultrasonic-assisted ionic liquid method for extracting polysaccharide from M.lasiocarp has the advantages of high extraction yield and good in vitro activity. This method demonstrates good application prospects and great potential in the extraction of active substances from natural products.
Publication Date
9-25-2025
First Page
188
Last Page
196
DOI
10.13652/j.spjx.1003.5788.2025.80082
Recommended Citation
Qiuhong, DUAN and Qingchao, JIA
(2025)
"Extraction of polysaccharide from Musella lasiocarpa by ultrasonic-assisted ionic liquid method and study on the in vitro activity and monosaccharide composition of the polysaccharide,"
Food and Machinery: Vol. 41:
Iss.
8, Article 25.
DOI: 10.13652/j.spjx.1003.5788.2025.80082
Available at:
https://www.ifoodmm.cn/journal/vol41/iss8/25
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