Research progress in detection techniques of common pathogenic bacteria in aquatic products

Authors

XU Meijin, College of Food Science and Technology , Guangdong Ocean University , Zhanjiang , Guangdong 524088 , China
LIN Haisheng, College of Food Science and Technology , Guangdong Ocean University , Zhanjiang , Guangdong 524088 , China;Guangdong Key Laboratory of Aquatic Products Processing and Safety , Zhanjiang , Guangdong 524088 , China;Key Laboratory of Aquatic Products Deep Processing of Guangdong Universities , Zhanjiang , Guangdong 524088 , China;National Research and Development Center for Shellfish Processing Technology (Zhanjiang ), Zhanjiang , Guangdong 524088 , China;South China Sea Biological Resources Development and Utilization Collaborative Innovation Center , Zhanjiang , Guangdong 524088 , ChinaFollow
HUANG He, College of Food Science and Technology , Guangdong Ocean University , Zhanjiang , Guangdong 524088 , China;Guangdong Key Laboratory of Aquatic Products Processing and Safety , Zhanjiang , Guangdong 524088 , China;Key Laboratory of Aquatic Products Deep Processing of Guangdong Universities , Zhanjiang , Guangdong 524088 , China;National Research and Development Center for Shellfish Processing Technology (Zhanjiang ), Zhanjiang , Guangdong 524088 , China;South China Sea Biological Resources Development and Utilization Collaborative Innovation Center , Zhanjiang , Guangdong 524088 , China

Corresponding Author(s)

林海生（1985—），男，广东海洋大学副教授，博士。E-mail：haishenglin@163.com

Abstract

Aquatic products are an essential source of nutrition for humans, and their safety has attracted much attention due to the contaminations with pathogenic bacteria. Pathogenic bacteria such as Vibrio parahaemolyticus, Salmonella spp., Listeria monocytogenes, Escherichia coli, Staphylococcus aureus, and Shigella spp. could easily invade aquatic products through water contamination or cross-contamination during processing, causing foodborne diseases and economic losses. This article reviews the contamination routes and hazards of the major pathogenic bacteria in aquatic products, systematically summarizes the properties and application status of both conventional detection techniques (including selective medium method and plate counting method) and novel detection techniques, such as loop-mediated isothermal amplification and recombinase polymerase amplification.

Publication Date

9-25-2025

First Page

197

Last Page

205

DOI

10.13652/j.spjx.1003.5788.2025.80136

Recommended Citation

Meijin, XU; Haisheng, LIN; and He, HUANG (2025) "Research progress in detection techniques of common pathogenic bacteria in aquatic products," Food and Machinery: Vol. 41: Iss. 8, Article 26.
DOI: 10.13652/j.spjx.1003.5788.2025.80136
Available at: https://www.ifoodmm.cn/journal/vol41/iss8/26

References

[1] WHO.Media centre 2017:foodsafety [EB/OL ].(2017 -10-26)
[2024 -12-25].http://who.int/mediacentre/factsheets/fs 399/en/.
[2] 范鹏辉,李红秋,褚遵华,等.2023年中国大陆食源性疾病暴发监测结果分析 [J].中国食品卫生杂志,2024,36(10):1 199-1 208.FAN P H,LI H Q,ZHU Z H,et al.Analysis of foodborne diseases outbreak surveillance in China's Mainland,2023 [J].Chinese Journal of Food Hygiene,2024,36(10):1 199-1 208.
[3] ZHOU Q J,LU J F,SU X R,et al.Simultaneous detection of multiple bacterial and viral aquatic pathogens using a fluorogenic loop-mediated isothermal amplification-based dual-sample microfluidic chip [J].Journal of Fish Diseases,2021,44(4):401-413.
[4] 李红秋,郭云昌,刘志涛,等.2022年中国大陆食源性疾病暴发监测资料分析 [J].中国食品卫生杂志,2024,36(8):962-967.LI H Q,GUO Y C,LIU Z T,et al.Analysis of foodborne disease outbreaks in China's mainland in 2022 [J].Chinese Journal of Food Hygiene,2024,36(8):962-967.
[5] 孙健,张强,陈凤萍,等.一起副溶血性弧菌食物中毒事件溯源调查 [J].中国预防医学杂志,2021,22(3):238-240.SUN J,ZHANG Q,CHEN F P,et al.Traceability investigation of a Vibrio parahaemolyticus food poisoning incident [J].Chinese Preventive Medicine,2021,22(3):238-240.
[6] 赵燕妮,武若冰,余瑞,等.代谢组学在食源性致病弧菌研究中的应用进展 [J].食品与发酵工业,2023,49(22):311-317.ZHAO Y N,WU R B,YU R,et al.Application of metabolomics in the study of foodborne pathogenic Vibrio spp.[J].Food and Fermentation Industries,2023,49(22):311-317.
[7] IBRAHIM M M,JUSOH M B,FARID Z C R,et al.Salmonella serovars trend in poultry Malaysia from 2011 to 2020 [J].Veterinary Research Communications,2024,48(3):791-802.
[8] 卢奕,刘海泉,赵勇,等.食源性致病菌生长异质性对风险评估的影响 [J].食品与机械,2018,34(5):164-168.LU Y,LIU H Q,ZHAO Y,et al.The influence of foodborne pathogens growth heterogeneity on risk assessment [J].Food & Machinery,2018,34(5):164-168.
[9] LANGMEAD B,SALZBERG S L.Fast gapped-read alignment with bowtie 2[J].Nature Methods,2012,9(4):357-359.
[10] 陈平亚,何翠华,张亮亮,等.单核细胞增生李斯特菌 LAMP检测方法的建立及应用 [J].安徽农业科学,2023,51(10):74-78.CHEN P Y,HE C H,ZHANG L L,et al.Development of a |loop-mediated isothermal amplification method for rapid detection of listeria monocytogenes [J].Journal of Anhui Agricultural Sciences,2023,51(10):74-78.
[11] 陈健舜,江玲丽,吕永辉,等.水产品中单增李斯特菌的分子流行病学特征与致病力研究 [J].中国食品学报,2013,13(9):182-189.CHEN J S,JIANG L L,LV Y H,et al.Molecular epidemiology and pathogenic potential of Listeria monocytogenes in aquatic products [J].Chinese Journal of Food Science,2013,13(9):182-189.
[12] RAJAPAKSHA P,ELBOURNE A,GANGADOO S,et al.A review of methods for the detection of pathogenic microorganisms [J].The Analyst,2019,144(2):396-411.
[13] 黄欣悦,陈娟,马欣玥.食品中金黄色葡萄球菌定量风险评估的研究进展 [J].食品工业科技,2021,42(22):390-397.HUANG X Y,CHEN J,MA X Y.Research progress of quantitative risk assessment of Staphylococcus aureus in food[J].Science and Technology of Food Industry,2021,42(22):390-397.
[14] ALPHONSE N,ODENDALL C.Animal models of shigellosis:a historical overview [J].Current Opinion in Immunology,2023,85:102399.
[15] 张欣悦,杨钰娟,孔祥翔,等.重组酶介导的等温扩增结合CRISPR/Cas 12a检测志贺氏菌和克罗诺杆菌 [J].食品安全质量检测学报,2024,15(11):35-44.ZHANG X Y,YANG Y J,KONG X X,et al.Detection of shigella and cronobacter using recombinase-aided amplification combined with CRISPR/Cas 12a[J].Journal of Food Safety & Quality,2024,15(11):35-44.
[16] 许志瑛,厉君悄,夏苗苗,等.水产品金黄色葡萄球菌平板计数法结果影响因素分析 [J].乡村科技,2024,15(9):155-157.XU Z Y,LI J Q,XIA M M,et al.Analysis of factors affecting the results of Staphylococcus aureus plate counting method in aquatic products [J].Rural Science and Technology,2024,15(9):155-157.
[17] 李辉.进出口水产品中副溶血性弧菌平板计数定量检测方法研究 [Z].辽宁省,东港海关综合技术服务中心,2023 -12-14.LI H.Research on the quantitative detection method of Vibrio parahaemolyticus plate counting in import and export aquatic products [Z].Liaoning Province,Donggang Customs Comprehensive Technical Service Center,2023 -12-14.
[18] NORDMANN P,SADEK M,DE LA ROSA J M D,et al.Selective culture medium for screening of fosfomycin resistance in Enterobacterales [J].Journal of Clinical Microbiology,2022,60(1):e02063 -21.
[19] 魏琼,陈萍,刘道文,等.沙门菌、志贺菌和金葡菌复合增菌培养基研制 [J].中国公共卫生,2011,27(6):807-809.WEI Q,CHEN P,LIU D W,et al.Enrichment broth for simultaneous culture of Salmonella,Shigellae,and Staphylococcus aureu [J].Chinese Journal of Public Health,2011,27(6):807-809.
[20] 易青,宁喜斌.沙门菌、金黄色葡萄球菌、副溶血性弧菌的选择性共增菌培养基的研制 [J].中国食品卫生杂志,2023,35(4):510-516.YI Q,NING X B.Development of selective co-enrichment medium for Salmonella,Staphylococcus aureus and Vibrio parahaemolyticus [J].Chinese Journal of Food Hygiene,2023,35(4):510-516.
[21] OSLAN S N H,YUSOF N Y,LIM S J,et al.Rapid and sensitive detection of Salmonella in agro-food and environmental samples:a review of advances in rapid tests and biosensors [J].Journal of Microbiological Methods,2024,219:106897.
[22] WANG S,HOU R,BAO Z M,et al.Transcriptome sequencing of Zhikong scallop （Chlamys farreri ）and comparative transcriptomic analysis with Yesso scallop (Patinopecten yessoensis )[J].PLoS One,2013,8(5):e63927.
[23] JIN J S,LIU X D,SHIROGUCHI K.Long journey of 16S rRNA-amplicon sequencing toward cell-based functional bacterial microbiota characterization [J].iMetaOmics,2024,1(1):e9.
[24] 黎炯,叶星,卢迈新,等.双重 PCR快速鉴别无乳链球菌和海豚链球菌 [J].湖南农业大学学报 (自然科学版 ),2010,36(4):449-452.LI J,YE X,LU M X,et al.Rapid identification of Streptococus agalactiae and Streptococus iniae with duplex PCR assay [J].Journal of Hunan Agricultural University (Natural Sciences ),2010,36(4):449-452.
[25] 杨文鸽,孙翠玲,潘云娣,等.水产品中致病微生物的快速检测方法 [J].中国食品学报,2006,6(1):402-406.YANG W G,SUN C L,PAN Y D,et al.Rapid detection methods of pathogenic microorganism from aquatic foods [J].Journal of Chinese Institute of Food Science and Technology,2006,6(1):402-406.
[26] VOLOKHOV D,RASOOLY A,CHUMAKOV K,et al.Identification of listeria species by microarray-based assay [J].Journal of Clinical Microbiology,2002,40(12):4 720-4 728.
[27] 张国林,苏远科,沈海英,等.16S rRNA/ITS 基因序列分析与微生物分类鉴定及其在药品微生物质量控制中的应用 [J].中国现代应用药学,2017,34(10):1 489-1 495.ZHANG G L,SU Y K,SHEN H Y,et al.Application of 16S rRNA/ITS sequencing and analysis in the identification,classification of microbial and drug quality control [J].Chinese Modern Applied Pharmacy,2017,34(10):1 489-1 495.
[28] 叶乃芳,王中新.16S rRNA及相关技术用于临床细菌鉴定的研究进展 [J].国际检验医学杂志,2015,36(4):520-522.YE N F,WANG Z X.Research progress of 16S rRNA and related technologies in inical bacterial identification [J].International Journal of Laboratory Medicine,2015,36(4):520-522.
[29] 李荣荣.水产品中常见重要致病菌基因芯片技术的研究 [D].天津:南开大学,2011:20-22.LI R R.Research on Genomic chip technology for common important pathogenic bacteria in aquatic products [D].Tianjin:Nankai University,2011:20-22.
[30] 刘淑文,丁家旺,秦伟.海水中致病菌检测研究进展 [J].海洋科学,2017,41(2):145-156.LIU S,DING J W,QIN W.Research progress in detecting pathogenic bacteria in seawater [J].Marine Sciences,2017,41(2):145-156.
[31] NEOGI S B,CHOWDHURY N,ASAKURA M,et al.A highly sensitive and specific multiplex PCR assay for simultaneous detection of Vibrio cholerae,Vibrio parahaemolyticus and Vibrio vulnificus [J].Letters in Applied Microbiology,2010,51(3):293-300.
[32] 白瑶,李斌,李凤琴,等.双重实时荧光 PCR检测副溶血性弧菌毒力基因方法的建立和应用 [J].中国食品卫生杂志,2022,34(1):54-59.BAI Y,LI B,LI F Q,et al.Establishment and application of dual real-time PCR for detection of virulence genes of Vibrio parahaemolyticus [J].Chinese Journal of Food Hygiene,2022,34(1):54-59.
[33] 柳嘉欣.基于杂交链式反应的病原菌检测技术研究与应用[D].海口:海南大学,2023:43-46.LIU J X.Research and application of pathogen detection technology based on hybrid chain reaction [D].Haikou:Hainan University,2023:43-46.
[34] 白亚龙,索玉娟,周昌艳.食源性致病菌 PCR检测前处理方法研究进展 [J].食品与机械,2017,33(12):191-196.BAI Y L,SUO Y J,ZHOU Y C.A review of the development in pretreatment methods for PCR detection of food-borne pathogens [J].Food & Machinery,2017,33(12):191-196.
[35] 叶云,容元平.PCR技术检测食品有害微生物的应用 [J].食品与机械,2007,23(2):98-101,117.YE Y,RONG Y P.Applications of PCR in the field of foodborne pathogens [J].Food & Machinery,2007,23(2):98-101,117.
[36] 彭琳媛,林洪,王静雪.PMA-qPCR 定量检测 VBNC副溶血弧菌方法的建立与优化 [J].食品与发酵工业,2022,48(1):247-252.PENG L Y,LIN H,WANG J X.Development and optimization of PMA-qPCR for the quantitative detection of viable but non-culturable Vibrio parahaemolyticus [J].Food and Fermentation Industries,2022,48(1):247-252.
[37] 高林晨萌,叶华,黄圣博,等.核酸适配体在食品危害物多靶标检测中的应用进展 [J].食品与机械,2021,37(4):217-225.GAO L M C,YE H,HUANG S B,et al.Recent advances in simultaneous detection of food hazards based on aptasensor [J].Food & Machinery,2021,37(4):217-225.[38] 廖小艳,陈丽丽,白亚龙.食品中诺如病毒检测技术研究进展[J].食品与机械,2021,37(4):200-206.LIAO X Y,CHEN L L,BAI Y L.The progress of detection methods for norovirus in foods [J].Food & Machinery,2021,37(4):200-206.
[39] 李国,闫茂仓,常维山,等.文蛤副溶血弧菌间接 ELISA检测技术的研究 [J].海洋通报,2008,27(5):85-90.LI G,YAN M C,CHANG W S,et al.Study on indirect ELISA method for detecting Vibrio parahaemolyticus in hard clam meretrix meretrix linnaeus [J].Marine Science Bulletin,2008,27(5):85-90.
[40] DU Z H,LIN S H,LI J L,et al.Nano-gold-enhanced LAMP method for qualitative visual detection of Salmonella in milk[J].Mikrochimica Acta,2022,189(9):365.
[41] PROSSNER K M,SMALL H J,CARNEGIE R B,et al.Immunofluorescence visualization of polycyclic aromatic hydrocarbon mixtures in the eastern oyster crassostrea virginica[J].Environmental Toxicology and Chemistry,2023,42(2):475-480.
[42] 姚斐,寇运同,陈刚,等.间接免疫荧光抗体技术检测活的非可培养状态的副溶血弧菌 [J].海洋科学,2000,24(9):10-12.YAO F,KOU Y T,CHEN G,et al.Detection of viable but non-culturable Vibrio parahaemolyticus by indirect immunofluorescent antibody technique [J].Marine Sciences,2000,24(9):10-12.
[43] CHANG C R,VYKUNTA V S,LEE J H J,et al.SEED-Selection enables high-efficiency enrichment of primary T cells edited at multiple loci [J].Nature Biotechnology,2025,43(2):212-222.
[44] 张凡非,杉山宽治,西尾智裕,等.利用免疫磁珠法分离环境及食品中产生 TDH副溶血性弧菌的研究 [J].中国卫生监督杂志,2004,11(1):7-9.ZHANG F F,SHAN S K Z,XI W Z Y,et al.Study of isolating WTBXVibro parahaemolyticusWTBZ producing TDH from environmental materialsand food using immunomagnetic separation method [J].Chinese Journal of Health Inspection,2004,11(1):7-9.
[45] 朱芳茜,何扩,张秀媛,等.水产品中志贺氏菌双抗夹心ELISA检测方法建立与应用 [J].食品研究与开发,2021,42(4):156-160.ZHU F Q,HE K,ZHANG X Y,et al.Establishment and application of double antibody sandwich ELISA to detect shigella in aquatic [J].Food Research and Development,2021,42(4):156-160.
[46] WANG Z H,BU T,SHAO B,et al.Integration of a new generation of immunochromatographic assays with nanomaterials for point-of-care testing [J].Trends in Analytical Chemistry,2024,174:107220.
[47] CHEN Y K,YU G X,KANG H T,et al.Duplex in-one-tube detection of two important shrimp farming pathogens with |color indication using a method combining RPA and PfAgo [J].Aquaculture,2025,597:741949.
[48] 夏诗琪,徐超莲,刘道峰,等.胶体金免疫层析法联检食品中5种典型沙门氏菌模型的建立和优化 [J].食品科学,2014,35(22):154-158.XIA S Q,XU C L,LIU D F,et al.Preparation and optimization of colloidal gold strip for simultaneously detecting five typical salmonella in foods [J].Food Science,2014,35(22):154-158.
[49] 谢士嘉,王静,王振国.利用胶体金免疫层析技术快速定量检测单增李斯特菌方法的建立 [J].中国国境卫生检疫杂志,2010,33(2):126-129.XIE S J,WANG J,WANG Z G.Rapid and quantitative detection of listeria monocytogenes using colloid gold immunochromatographic method [J].Chinese Journal of Frontier Health and Quarantine,2010,33(2):126-129.
[50] ZHU N F,LI X S,LIU Y,et al.Dual amplified ratiometric fluorescence ELISA based on G-quadruplex/hemin DNAzyme using tetrahedral DNA nanostructure as scaffold for ultrasensitive detection of dibutyl phthalate in aquatic system[J].Science of the Total Environment,2021,784:147212.
[51] 李宏,付冠艳,付淑君,等.胶体金免疫层析技术在食源性致病 菌 快 速 检 测 中 的 应 用 [J].食 品 与 机 械,2013,29(3):261-264.LI H,FU G Y,FU S J,et al.Application of colloidal gold immunochromatography technique for rapid detection of food-borne pathogens [J].Food & Machinery,2013,29(3):261-264.
[52] NOTOMI T,OKAYAMA H,MASUBUCHI H,et al.Loop-mediated isothermal amplification of DNA [J].Nucleic Acids Research,2000,28(12):E63.
[53] 徐芊,孙晓红,赵勇,等.利用环介导等温扩增技术快速检测水产品中的副溶血弧菌 [J].农业生物技术学报,2008,16(5):780-786.XU Q,SUN X H,ZHAO Y,et al.Rapid detection of vibrio parahaemolyticus in seafood using loop-mediated isothermal amplification (LAMP ) method [J].Journal of Agricultural Biotechnology,2008,16(5):780-786.
[54] POON L L M,LEUNG C S W,TASHIRO M,et al.Rapid detection of the severe acute respiratory syndrome (SARS ) coronavirus by a loop-mediated isothermal amplification assay[J].Clinical Chemistry,2004,50(6):1 050-1 052.
[55] 高玮.副溶血弧菌常规调查及其 3种基因（tlh、tdh及trh）LAMP检测方法的研究 [D].上海:上海海洋大学,2011:3-6.GAO W.Rroutine investigation of Vibrio parahaemolycus and detection of 3 kinds of gene in Vibrio parahaemolycus by Loop mediated isothermal amplification (LAMP ) [D].Shanghai:Shanghai Ocean University,2011:3-6.
[56] TAN M Y,LIAO C,LIANG L N,et al.Recent advances in recombinase polymerase amplification:principle,advantages,disadvantages and applications [J].Frontiers in Cellular and Infection Microbiology,2022,12:1019071.
[57] 李达容.水产品中副溶血性弧菌和霍乱弧菌 RAA-LFD 快速检测方法的建立与应用研究 [D].上海:上海海洋大学,2022:33-34.LI D.R.Establishment and application of rapid detection methods for Vibrio parahaemolyticus and Vibrio cholerae using RAA-LFD in aquatic products [D].Shanghai:Shanghai Ocean University,2022:33-34.
[58] GENG Y Y,TAN K,LIU L B,et al.Development and evaluation of a rapid and sensitive RPA assay for specific detection of Vibrio parahaemolyticus in seafood [J].BMC Microbiology,2019,19(1):186.
[59] 姜侃,张慧,汪新,等.多重 LAMP-熔解曲线法检测食品中两种食源性致病菌 [J].食品与机械,2015,31(2):87-92.JIANG K,ZHANG H,WANG X,et al.Development of multiplex LAMP combined with melting curves analysis for detection of two kinds of food borne pathogens [J].Food & Machinery,2015,31(2):87-92.
[60] 唐毓祎.水产品弧菌的等温核酸扩增可视化检测方法的建立与应用 [D].上海:上海海洋大学,2017:27-28.TANG Y Y.Development and application ofcolorimetric detection of Vibrio inseafood based on isothermalamplification techniques [D].Shanghai:Shanghai Ocean University,2017:27-28.