Abstract
[Objective] To establish a new Sin-QuEChERS nano-triple quadrupole liquid chromatography-mass spectrometry (UPLC-MS/MS) method for detecting emamectin benzoate residues in rice. [Methods] After extraction with 0.1% formic acid-acetonitrile, the sample is purified with N-primary secondary amine (PSA) and multi-walled carbon nanotube (MWCNTs) fillers. The mobile phase is a gradient elution of 0.1% formic acid water and acetonitrile. Separation is performed using a C18 chromatographic column, and the detection is carried out using a triple quadrupole liquid chromatography-tandem mass spectrometry system. [Results] The quantification limit of emamectin benzoate is 0.001 5 mg/kg, and the detection limit of the method is 0.000 5 mg/kg. A good linear relationship is observed for emamectin benzoate in the concentration range of 1~100 ng/mL, with a correlation coefficient greater than 0.999 5. At 2, 10, and 50 µg/kg spiking levels, the average recovery rate of emamectin benzoate is 86.9%~110.2%, with a relative standard deviation of 0.98%~7.21%. [Conclusion] This method is efficient, simple, stable, and highly sensitive, making it suitable for detecting emamectin benzoate in rice.
Publication Date
9-25-2025
First Page
61
Last Page
65
DOI
10.13652/j.spjx.1003.5788.2024.80939
Recommended Citation
Kuanneng, LUO; Zijie, HUANG; Pengmian, HUANG; Yong, LIU; Shuping, YANG; and Honggang, ZHANG
(2025)
"Determination of residual emamectin benzoate in rice by Sin-QuEChERS Nano-UPLC-MS/MS,"
Food and Machinery: Vol. 41:
Iss.
8, Article 8.
DOI: 10.13652/j.spjx.1003.5788.2024.80939
Available at:
https://www.ifoodmm.cn/journal/vol41/iss8/8
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