Abstract
[Objective] To investigate the effects of grinding size and extraction temperature on the chemical composition, colloidal properties, and antioxidant activity of coffee colloidal particles, and to elucidate their formation and characteristics. [Methods] Coffee infusions were prepared from whole beans, medium-ground (0.9 mm), and fine-ground (0.3 mm) coffee beans using hot brewing (100 ℃, 3 min) and cold brewing (4 ℃, 24 h). The content of total solids, total sugars, proteins, total polyphenols, and lipids were determined. The colloidal properties were characterized using a nanoparticle size and zeta potential analyzer. The infusions were separated into a filtrate (true solution phase) and a retentate (colloidal phase) using an ultrafiltration membrane with a nominal molecular weight cut-off of 1×105. The chemical composition and antioxidant activity of each phase were analyzed separately. Extracellular antioxidant activity was evaluated using ORAC, ABTS, and DPPH assays, while intracellular antioxidant activity was assessed using the CAA assay. [Results] Fine grinding (0.3 mm) combined with hot brewing significantly increased the dissolution rates of total solids, polyphenols, and lipids (P<0.05) and promoted the formation of colloidal particles (light scattering intensity of hot brew: 115 501.8 kcps, far exceeding 1 250.2 kcps for cold brew). Ultrafiltration showed that lipids and other components were enriched in the colloidal particles. The true solution phase (filtrate) primarily contributed to extracellular antioxidant activity, while the colloidal phase (retentate) exhibited significant biological activity in the CAA assay. [Conclusion] High-temperature extraction and fine grinding significantly enhance the formation of colloidal particles by promoting the dissolution and self-assembly of components. These colloidal particles constitute the key material basis for the intracellular antioxidant activity of coffee.
Publication Date
10-28-2025
First Page
1
Last Page
7
DOI
10.13652/j.spjx.1003.5788.2025.80654
Recommended Citation
Guanzhen, GAO; Pingfan, RAO; Yao, XU; Jianwu, ZHOU; and Yunhui, SHAN
(2025)
"Effects of coffee preparation processes on colloidal particle formation and antioxidant properties,"
Food and Machinery: Vol. 41:
Iss.
9, Article 1.
DOI: 10.13652/j.spjx.1003.5788.2025.80654
Available at:
https://www.ifoodmm.cn/journal/vol41/iss9/1
References
[1] BARREA L,PUGLIESE G,FRIAS-TORAL E,et al.Coffee consumption,health benefits and side effects:a narrative review and update for dietitians and nutritionists [J].Critical Reviews in Food Science and Nutrition,2023,63(9):1 238-1 261.
[2] GAO G Z,WANG H Q,ZHOU J W,et al.Isolation and characterization of bioactive proteoglycan-lipid nanoparticles from freshwater clam (Corbicula fluminea muller ) soup [J].Journal of Agricultural and Food Chemistry,2021,69(5):1 610-1 618.
[3] HAN H,KE L J,XU W,et al.Incidental nanoparticles in black tea alleviate DSS-induced ulcerative colitis in BALB/c mice [J].Food & Function,2023,14(18):8 420-8 430.
[4] GAO G Z,ZHOU J W,JIN Y Y,et al.Nanoparticles derived from porcine bone soup attenuate oxidative stress-induced intestinal barrier injury in Caco- 2 cell monolayer model [J].Journal of Functional Foods,2021,83:104573.
[5] HAN H,KE L J,WANG H Q,et al.Incidental nanoparticles in black tea infusion:carriers of bioactives fortifying protection on intestinal mucosal cells against oxidative stresses [J].Food Biophysics,2022,17:209-220.
[6] WANG H Q,HUANG J,DING Y N,et al.Nanoparticles isolated from porcine bone soup ameliorated dextran sulfate sodium-induced colitis and regulated gut microbiota in mice [J].Frontiers in Nutrition,2022,9:821404.
[7] SHENG S Q,FANG Z N,YANG H J,et al.Simultaneouslysuppressing the coffee ring effect of solutes with different sizes[J].The Journal of Physical Chemistry B,2023,127(49):10 615-10 623.
[8] MACHADO F,COIMBRA M A,DEL CASTILLO M D,et al.Mechanisms of action of coffee bioactive compounds - a key to unveil the coffee paradox [J].Critical Reviews in Food Science and Nutrition,2024,64(28):10 164-10 186.
[9] WANG X J,LIM L T.Effects of grind size,temperature,and brewing ratio on immersion cold brewed and French press hot brewed coffees [J].Applied Food Research,2023,3(2):100334.
[10] YUE F F,ZHANG J R,XU J X,et al.Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method [J].Frontiers in Nutrition,2022,9:963318.
[11] KARIMI F,HAMIDIAN Y,BEHROUZIFAR F,et al.An applicable method for extraction of whole seeds protein and its determination through Bradford's method [J].Food and Chemical Toxicology,2022,164:113053.
[12] LAWAG I L,NOLDEN E S,SCHAPER A A M,et al.A modified folin-ciocalteu assay for the determination of total phenolics content in honey [J].Applied Sciences,2023,13(4):2 135.
[13] 杨津淋.食品中脂肪测定国家标准方法的改良应用 [J].食品安全导刊,2022 (35):111-113,128.YANG J L.Improvement and application of national standard method for determination of fat in food [J].China Food Safety Magazine,2022 (35):111-113,128.
[14] SKROZA D,ŠIMAT V,VRDOLJAK L,et al.Investigation of antioxidant synergisms and antagonisms among phenolic acids in the model matrices using FRAP and ORAC methods [J].Antioxidants,2022,11(9):1 784.
[15] RUMPF J,BURGER R,SCHULZE M.Statistical evaluation of DPPH,ABTS,FRAP,and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins [J].International Journal of Biological Macromolecules,2023,233:123470.
[16] WANG H Q,SONG B B,ZHOU J W,et al.Fabrication and characterization of curcumin-loaded nanoparticles using licorice protein isolate from Radix Glycyrrhizae [J].International Journal of Biological Macromolecules,2024,255:128235.
[17] BARREAR LÓPEZ J A,HERNANDEZ CARRIÓN M.Functional properties and sensory profile of coffee prepared by different brewing methods [J].Food Science and Technology International,2025,31(3):248-260.
[18] KHAMITOVA G,ANGELONI S,BORSETTA G,et al.Optimization of espresso coffee extraction through variation of particle sizes,perforated disk height and filter basket aimed at lowering the amount of ground coffee used [J].Food Chemistry,2020,314:126220.
[19] CÓRDOBA N,MORENO F L,OSORIO C,et al.Chemical and sensory evaluation of cold brew coffees using different roasting profiles and brewing methods [J].Food Research International,2021,141:110141.
[20] TAIP F S,ABDUL GHANI N H,OTHMAN N.Empirical modelling,simulation and control of coffee brewing [J].Food Research,2025,9(2):32-39.
[21] CORDOBA N,FERNANDEZ-ALDUENDA M,MORENO F L,et al.Coffee extraction:a review of parameters and their influence on the physicochemical characteristics and flavour of coffee brews [J].Trends in Food Science & Technology,2020,96:45-60.
[22] PHAM A C,CLULOW A J,BOYD B J.Formation of self-assembled mesophases during lipid digestion [J].Frontiers in Cell and Developmental Biology,2021,9:657886.
[23] MIDEKESSA G,GODAKUMARA K,ORD J,et al.Zeta potential of extracellular vesicles:toward understanding the attributes that determine colloidal stability [J].ACS Omega,2020,5(27):16 701-16 710.
[24] WANG X J,LIM L T.Modeling study of coffee extraction at different temperature and grind size conditions to better understand the cold and hot brewing process [J].Journal of Food Process Engineering,2021,44(8):e13748.
[25] LI Y R,HUANG Y F,LU X K,et al.Criteria of distribution transitions in dispersed multiphase systems based on an extended lattice model [J].Langmuir,2023,39(48):17 021-17 030.
[26] SILVA A C R,GARRETT R,REZENDE C M.A workflow for lipid annotation in coffee samples by liquid chromatography[M]// KOOLEN H.Mass Spectrometry for Food Analysis.New York:Springer US,2022:71-87.
[27] FENG J L,BERTON-CARABIN C C,GUYOT S,et al.Coffee melanoidins as emulsion stabilizers [J].Food Hydrocolloids,2023,139:108522.
[28] WOŁOSIAK R,PAKOSZ P,DRUŻYŃSKA B,et al.Antioxidant activity of coffee components influenced by roast degree and preparation method [J].Applied Sciences,2023,13(4):2 057.
[29] WU J Y,XIONG S W,HUANG Z L,et al.Ultrasound modifies the properties of sodium alginate:correlation between biological activities and molecular characteristics [J].Food Biophysics,2025,20(3):112.
[30] WANG X R,WANG W X.Cellular journey of nanomaterials:theories,trafficking,and kinetics [J].Aggregate,2023,4(6):e372.
[31] XU L G,DUAN M C,CAI Z X,et al.Colloidal nanoparticles isolated from duck soup exhibit antioxidant effect on macrophages and enterocytes [J].Foods,2023,12(5):981.
[32] FLEMING E,LUO Y C.Co-delivery of synergistic antioxidants from food sources for the prevention of oxidative stress [J].Journal of Agriculture and Food Research,2021,3:100107.