Optimization of extraction and purification process of polyphenols from Prunus cerasifera Ehrhart and analysis of their antioxidant activity

Authors

AYIJIA Malike, School of Food Science and Pharmacy , Xinjiang Agricultural University , Urumqi , Xinjiang 830052 , China
SALAMET Adirs, Institute of Agro-products Processing , Xinjiang Academy of Agricultural Sciences , Urumqi , Xinjiang 830091 , China;Xinjiang Key Laboratory of Processing and Preservation of Agricultural Products , Urumqi , Xinjiang 830091 , China
TAJIGULI Abulikemu, School of Life Sciences , Xinjiang Normal University , Urumqi , Xinjiang 830054 , China;Xinjiang Key Laboratory of Species Conservation and Regulatory Biology , Urumqi , Xinjiang 830054 , China
ATAWULA Tiemuer, Institute of Agro-products Processing , Xinjiang Academy of Agricultural Sciences , Urumqi , Xinjiang 830091 , China;Xinjiang Key Laboratory of Processing and Preservation of Agricultural Products , Urumqi , Xinjiang 830091 , China
GULFIRA Ylham, Institute of Agro-products Processing , Xinjiang Academy of Agricultural Sciences , Urumqi , Xinjiang 830091 , China;Xinjiang Key Laboratory of Processing and Preservation of Agricultural Products , Urumqi , Xinjiang 830091 , China
NIGEREYI Yadikaer, School of Food Science and Pharmacy , Xinjiang Agricultural University , Urumqi , Xinjiang 830052 , ChinaFollow

Corresponding Author(s)

尼格尔热依·亚迪卡尔（1987—），女，新疆农业大学副教授，博士。E-mail：nigary-sy@xjau.edu.cn

Abstract

[Objective] Using the fruits of Prunus cerasifera as raw materials, this study aimed to optimize the preparation process of polyphenols and evaluate their antioxidant activity. [Methods] The optimal extraction conditions for polyphenols from P.cerasifera were determined using ultrasound-assisted compound enzyme extraction, optimized through single-factor experiments and a Box-Behnken response surface design. Macroporous adsorption resin was employed for the enrichment and purification of the polyphenols. The DPPH radical scavenging ability, ABTS ⁺ radical scavenging ability, and total antioxidant capacity of the polyphenols before and after purification were evaluated. [Results] The optimal extraction conditions were as follows:pectinase to cellulase mass ratio of 1∶2, enzyme addition of 1.5%, solid-liquid ratio of 1∶29 (g/mL), ultrasonic time of 37 min, and ultrasonic temperature of 60 ℃, resulting in a maximum polyphenol yield of (12.82±0.43) mg/g.HPD-300 was identified as the optimal purification resin through static adsorption-desorption screening, with adsorption equilibrium reached in 2 hours and desorption equilibrium in 0.5 hours. The optimal purification parameters using HPD-300 resin were as follows:sample concentration 100 mg/mL, sample volume 180 mL, flow rate 120 mL/h, ethanol concentration 60%, and eluent volume 210 mL. After purification with HPD-300, the IC50 values of the polyphenols for DPPH and ABTS ⁺ radicals were 43.16 times and 16.00 times higher than those before purification, respectively, and the total antioxidant capacity was significantly enhanced. [Conclusion] The combination of ultrasound-assisted compound enzyme extraction and purification using HPD-300 macroporous adsorption resin enables efficient enrichment and purification of polyphenols from P.cerasifera, and significantly improves their antioxidant activity.

Publication Date

10-28-2025

First Page

169

Last Page

178

DOI

10.13652/j.spjx.1003.5788.2024.81059

Recommended Citation

Malike, AYIJIA; Adirs, SALAMET; Abulikemu, TAJIGULI; Tiemuer, ATAWULA; Ylham, GULFIRA; and Yadikaer, NIGEREYI (2025) "Optimization of extraction and purification process of polyphenols from Prunus cerasifera Ehrhart and analysis of their antioxidant activity," Food and Machinery: Vol. 41: Iss. 9, Article 22.
DOI: 10.13652/j.spjx.1003.5788.2024.81059
Available at: https://www.ifoodmm.cn/journal/vol41/iss9/22

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