Abstract
[Objective] To address the limitation of spectral overlap interference in the rapid detection of 3, 4-dihydroxyphenylethanol (DHPET) within the 4-hydroxyphenethyl alcohol (HPET) catalytic system using traditional ultraviolet spectrophotometry. [Methods] To overcome the insufficient specificity of existing methods, a rapid spectrophotometric detection method based on Fe3+ coordination modulation was proposed. Through bidentate chelation between Fe3+ and the catechol group of DHPET, the characteristic absorption spectrum was induced to undergo a bathochromic shift to 760 nm, significantly distinguishing it from the weak coordination response of monophenolic compounds. [Results] After optimization, the method exhibited a linear range of 3~100 mmol/L (R2=0.998), a detection limit of 0.709 mmol/L, and precision with RSD <2%, meeting the requirements for real-time monitoring in industrial catalytic systems. [Conclusion] The molecular mechanism of enhanced selectivity through bidentate coordination is practical in enzymatic catalytic systems, providing a low-cost, rapid analytical tool for the industrial production of DHPET.
Publication Date
10-28-2025
First Page
42
Last Page
51
DOI
10.13652/j.spjx.1003.5788.2025.80290
Recommended Citation
Junhan, ZHOU; Jianxian, ZHENG; and Lu, SUN
(2025)
"Rapid detection of 3,4-dihydroxyphenylethanol by ultraviolet spectrophotometry based on Fe3+ coordination modulation,"
Food and Machinery: Vol. 41:
Iss.
9, Article 6.
DOI: 10.13652/j.spjx.1003.5788.2025.80290
Available at:
https://www.ifoodmm.cn/journal/vol41/iss9/6
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