Effect of key NRPS genes on synthesis of the active peptide DLAP in Ganoderma sinense

Authors

XIAO Zhenyan, Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry and Technology, Changsha, Hunan 410004, China; International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology of Hunan Province, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
WU Qiang, Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry and Technology, Changsha, Hunan 410004, China; International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology of Hunan Province, Central South University of Forestry and Technology, Changsha, Hunan 410004, China; Hunan Key Laboratory of Ecological Winemaking Technology and Application, Shaoyang University, Shaoyang, Hunan 422000, ChinaFollow
WANG Xiaoling, Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry and Technology, Changsha, Hunan 410004, China; International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology of Hunan Province, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
HUANG Jia, Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry and Technology, Changsha, Hunan 410004, China; International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology of Hunan Province, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
WANG Haojin, Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry and Technology, Changsha, Hunan 410004, China; International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology of Hunan Province, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
LIU Gaoqiang, Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry and Technology, Changsha, Hunan 410004, China; International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology of Hunan Province, Central South University of Forestry and Technology, Changsha, Hunan 410004, China

Corresponding Author(s)

伍强（1989—），女，邵阳学院副教授，博士。E-mail：qiangwusmile@163.com

Abstract

[Objective] To explore the key non-ribosomal peptide synthetase (NRPS) genes of the active peptide Asp-Leu-Ala-Pro (DLAP) with angiotensin-converting enzyme (ACE) inhibitory activity identified from the mycelia of Ganoderma sinense. [Methods] The effects of key NRPS genes on the synthesis of DLAP are analyzed by PEG-mediated genetic transformation experiment, real-time fluorescence quantitative PCR (qRT-PCR), and high performance liquid chromatography (HPLC). [Results] qRT-PCR results reveal that compared with the wild type, the silenced transformants of GSI_00900 have the reductions of 44.99%, 68.88%, and 66.17% in the expression level of this gene. The three silenced transformants of GSI_ 10299 show the reductions of 74.11%, 78.01%, and 75.76% in the expression level of this gene. The two silenced transformants of GSI_10830 present reductions of 30.55% and 76.99%, and those of GSI_11441 exhibit the expression declines ranging from 19.43% to 84.80%. HPLC results indicate that the DLAP content in each positive transformant was lower than that in the wild type (P<0.05). [Conclusion] These four NRPS genes (GSI_00900, GSI_10299, GSI_10830, and GSI_11441) play a crucial role in the synthesis of DLAP.

Publication Date

1-23-2026

First Page

111

Last Page

119

DOI

10.13652/j.spjx.1003.5788.2025.80133

Recommended Citation

Zhenyan, XIAO; Qiang, WU; Xiaoling, WANG; Jia, HUANG; Haojin, WANG; and Gaoqiang, LIU (2026) "Effect of key NRPS genes on synthesis of the active peptide DLAP in Ganoderma sinense," Food and Machinery: Vol. 42: Iss. 1, Article 15.
DOI: 10.13652/j.spjx.1003.5788.2025.80133
Available at: https://www.ifoodmm.cn/journal/vol42/iss1/15

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