Effects of honey compounded with Colla Corii Asini powder on antioxidant capacity and intestinal flora of mouse model

Authors

LI Cong, Dong 'e Ejiao Health Product Co ., Ltd., Liaocheng, Shandong 252200, China; Liaocheng Key Laboratory of Functional Food Research and Development, Liaocheng, Shandong 252200, China; Liaocheng Engineering Research Center for Nutrition and Health Consumer Products, Liaocheng, Shandong 252200, China
LU Xiaodong, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu 214122, China; State Key Laboratory of Food Science and Resources, Wuxi, Jiangsu 214122, China
MA Xueying, Dong 'e Ejiao Health Product Co ., Ltd., Liaocheng, Shandong 252200, China; Liaocheng Key Laboratory of Functional Food Research and Development, Liaocheng, Shandong 252200, China; Liaocheng Engineering Research Center for Nutrition and Health Consumer Products, Liaocheng, Shandong 252200, China
WANG Teng, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu 214122, China; State Key Laboratory of Food Science and Resources, Wuxi, Jiangsu 214122, China
XIE Xiao, Dong 'e Ejiao Health Product Co ., Ltd., Liaocheng, Shandong 252200, China; Liaocheng Key Laboratory of Functional Food Research and Development, Liaocheng, Shandong 252200, China; Liaocheng Engineering Research Center for Nutrition and Health Consumer Products, Liaocheng, Shandong 252200, China
ZHANG Jianling, Dong 'e Ejiao Health Product Co ., Ltd., Liaocheng, Shandong 252200, China; Liaocheng Key Laboratory of Functional Food Research and Development, Liaocheng, Shandong 252200, China; Liaocheng Engineering Research Center for Nutrition and Health Consumer Products, Liaocheng, Shandong 252200, China
WANG Zhouping, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu 214122, China; State Key Laboratory of Food Science and Resources, Wuxi, Jiangsu 214122, ChinaFollow

Corresponding Author(s)

王周平（1974—），男，江南大学教授，博士。E-mail：wangzp@jiangnan.edu.cn

Abstract

[Objective] To explore the regulatory effects and changing characteristics of the compound of Colla Corii Asini powder and honey on the antioxidant activity and intestinal flora of mice. [Methods] Seventy male BALB/c mice of SPF (Specific Pathogen Free, SPF) |grade are randomly divided into 7 groups (10 mice in each group), namely the blank control group, the model control group, the Colla Corii Asini group (0.5 g/kg BW), the low/high-dose honey groups (1.0/2.0 g/kg BW, respectively), and the low/high-dose compound groups (0.5+1.0/0.5+2.0 g/kg BW, respectively). Except for the blank group, oxidative stress models are established for the remaining groups. After 30 days of continuous intervention, relevant indicators are detected. [Results] Compared with the Colla Corii Asini group and the honey group, the high-dose compound group significantly reduces the levels of lipid peroxidation products (MDA) and protein carbonylation (PCO), while simultaneously increasing the levels of superoxide dismutase (SOD) and reduced glutathione (GSH) (P<0.05), confirming that the compound has a synergistic antioxidant effect, and the high-dose group exhibits the best effect. The compound alleviates the body's inflammatory response by inhibiting the levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). The results of genomic sequencing of the microbial flora in the intestinal contents show that the effects of sole intake of Colla Corii Asini or honey on the flora were not significant, while the intake of the compound of Colla Corii Asini and honey could improve microecological disorders and imbalances, with the high-dose compound demonstrating a better effect. In the intestinal flora of mice that ingested the low-dose and high-dose compounds, there is an increase in the content of Bacteroidetes and a decrease in the content of Firmicutes. [Conclusion] The compound of Colla Corii Asini and honey effectively enhances the body's free radical scavenging ability through its synergistic antioxidant activity. Meanwhile, it regulates the balance of the flora, significantly reduces oxidative stress damage, and contributes to maintaining the homeostasis of the intestinal microenvironment.

Publication Date

1-23-2026

First Page

120

Last Page

129

DOI

10.13652/j.spjx.1003.5788.2025.80746

Recommended Citation

Cong, LI; Xiaodong, LU; Xueying, MA; Teng, WANG; Xiao, XIE; Jianling, ZHANG; and Zhouping, WANG (2026) "Effects of honey compounded with Colla Corii Asini powder on antioxidant capacity and intestinal flora of mouse model," Food and Machinery: Vol. 42: Iss. 1, Article 16.
DOI: 10.13652/j.spjx.1003.5788.2025.80746
Available at: https://www.ifoodmm.cn/journal/vol42/iss1/16

References

[1] WANG Z X,WANG Q Q,TANG F Y,et al.Composite dietary antioxidant index and obesity among U.S.adults in NHANES 2007 —2018 [J].Scientific Reports,2024,14:28102.
[2] PARK J,TANG H,ZHANG P.Deciphering the antioxidant capacity of common vitamins against specific reactive oxygen species by nuclear magnetic resonance [J].Food Chemistry,2025,475:143184.
[3] ZHANG Q Q,SUN Z,SUN W B,et al.Engineering a synergistic antioxidant inhibition nanoplatform to enhance oxidative damage in tumor treatment [J].Acta Biomaterialia,2023,158:625-636.
[4] MZID M,BADRAOUI R,BEN KHEDIR S,et al.Protective effect of ethanolic extract of Urtica urens L.against the toxicity of imidacloprid on bone remodeling in rats and antioxidant activities [J].Biomedicine & Pharmacotherapy,2017,91:1 022-1 041.
[5] ZHAO L L,SUN J L,LIANG J,et al.Enhancing lipid metabolism and inducing antioxidant and immune responses to adapt to acute hypoxic stress in Schizothorax prenanti [J].Aquaculture,2020,519:734933.
[6] CHEN G P,WANG Y Y,LIU X B,et al.Enhancing the effects of curcumin on oxidative stress injury in brain vascular endothelial cells using lactoferrin peptide nano-micelles:antioxidant activity and mechanism [J].Journal of the Science of Food and Agriculture,2025,105(1):372-381.
[7] POYATOS P,GRATACOS M,SAMUEL K,et al.Oxidative stress and antioxidant therapy in pulmonary hypertension [J].Antioxidants,2023,12(5):1 006.
[8] LU S Y,LIU M L,LIU H,et al.Gestational exposure to bisphenol AF causes endocrine disorder of corpus luteum by altering ovarian SIRT- 1/Nrf2/NF-kB expressions and macrophage proangiogenic function in mice [J].Biochemical Pharmacology,2024,220:115954.
[9] 凌荣瑞.堇叶碎米荠富硒多肽对乙醇诱导肝细胞损伤的保护作用 [D].无锡:江南大学,2023:8-12.LING R R.Protective effect of selenium-enriched peptide from Cardamine violifolia on ethanol-induced hepatocyte injury [D].Wuxi:Jiangnan University,2023:8-12.
[10] 杨晓,王畋,刘畅,等.低聚肽和 VC复合固体饮料的抗氧化性研究 [J].食品研究与开发,2018,39(4):179-183.YANG X,WANG T,LIU C,et al.The antioxidant properties of oligopeptide and VC compound solid drink [J].Food Research and Development,2018,39(4):179-183.
[11] 陈娜.高F值寡肽与药食同源食品组分联合解酒护肝机制初步研究及产品开发 [D].烟台:鲁东大学,2024:10-13.CHEN N.Study on the mechanism and product development of high fischer ratio oligopeptides combined with medicinal and edible homologous food components to relieve alcohol and protect liver [D].Yantai:Ludong University,2024:10-13.
[12] SEMA D Ç,GÜLAY Ö,ASLI N A,et al.Biomarkers of oxidative stress and antioxidant defense [J].Journal of Pharmaceutical and Biomedical Analysis,2022,209:114477.
[13] 李聪,季萌萌,孟苓凤,等.阿胶和阿胶糕不同极性溶剂提取物抗皮肤衰老功效探究 [J].食品与发酵工业,2025,51(17):82-92.LI C,JI M M,MENG L F,et al.Study on anti-skin aging effect of different polar solvent extracts of Colla corii asini and Ejiao cake [J].Food and Fermentation Industries,2025,51(17):82-92.
[14] 陈瑛琪,任世利,刘广志,等.阿胶多肽的组成及功能研究进展[J].山东农业大学学报 (自然科学版 ),2024,55(2):288-294.CHEN Y Q,REN S L,LIU G Z,et al.Research progress on composition and function of colla corii asini polypeptide [J].Journal of Shandong Agricultural University (Natural Science Edition ),2024,55(2):288-294.
[15] 段小波,杜芬,王延涛,等.阿胶和阿胶肽对血管内皮细胞氧化损伤的作用研究 [J].安徽农业科学,2025,53(8):162-167,180.DUAN X B,DU F,WANG Y T,et al.Study on effects of ejiao and ejiao peptide on oxidative injury of vascular endothelial cells [J].Journal of Anhui Agricultural Sciences,2025,53(8):162-167,180.
[16] 梁荣,徐乐,樊琛,等.酶解法制备阿胶蛋白肽工艺条件优化及抗氧化活性分析 [J].食品工业科技,2024,45(10):217-224.LIANG R,XU L,FAN C,et al.Optimization of enzymatic hydrolysis process for preparing peptides from Asini corii colla and its antioxidant activity [J].Science and Technology of Food Industry,2024,45(10):217-224.
[17] 樊雨梅,汝文文,史传超,等.阿胶低聚肽的成分分析及其抗氧化活性 [J].食品工业科技,2020,41(18):314-318,323.FAN Y M,RU W W,SHI C C,et al.Composition and its antioxidant activity of Colla corii asini oligopeptides [J].Science and Technology of Food Industry,2020,41(18):314-318,323.
[18] XU X B,GUO S W,HAO X H,et al.Improving antioxidant and antiproliferative activities of Colla corii asini hydrolysates using ginkgo biloba extracts [J].Food Science & Nutrition,2018,6(4):765-772.
[19] 杜博玮,徐晓冰,郭尚伟,等.高抗氧化性小分子阿胶的研究[J].北京化工大学学报 (自然科学版 ),2019,46(6):15-20.DU B W,XU X B,GUO S W,et al.Preparation of a high-antioxidant hydrolysate from Colla corii asini [J].Journal of Beijing University of Chemical Technology (Natural Science Edition ),2019,46(6):15-20.
[20] 王会迪.不同成熟阶段椴树蜜的成分分析及其体外抗氧化和抑菌作用的研究 [D].延吉:延边大学,2022:7-9.WANG H D.Component analysis of linden honey at different maturity stages and its antioxidant and antibacterial effects in vitro [D].Yanji:Yanbian University,2022:7-9.
[21] 尚方园,潘丽,谷克仁,等.橄榄叶多酚提取工艺优化及对山茶籽油氧化稳定性的影响 [J].食品与机械,2022,38(9):146-152.SHANG F Y,PAN L,GU K R,et al.Optimization of extraction process of polyphenols from olive leaves and its |effect on oxidative stability of camellia seed oil [J].Food & Machinery,2022,38(9):146-152.
[22] 肖星凝,袁娅,廖霞,等.食用菌抗氧化活性成分及其抗氧化作用机制研究进展 [J].食品与机械,2016,32(11):207-211.XIAO X N,YUAN Y,LIAO X,et al.Research advance on antioxidant active composition and antioxidant mechanisms of edible fungi [J].Food & Machinery,2016,32(11):207-211.
[23] LIU R J,ZHANG X H,LIU H H,et al.Revealing the key antioxidant compounds and potential action mechanisms of Bauhinina championii honey based on non-targeted metabolomics,mineralogical analysis and physicochemical characterization [J].Food Chemistry,2025,477:143456.
[24] FAUZI A N,NORAZMI M N,YAACOB N S.Tualang honey induces apoptosis and disrupts the mitochondrial membrane potential of human breast and cervical cancer cell lines [J].Food Chem Toxicol,2011,49(4):871-878.
[25] 张金萍.山乌桕蜜改善小鼠酒精肝损伤及肠道菌群的作用和机制 [D].南昌:南昌大学,2022:4-5.ZHANG J P.The mechanism of Triadica cochinchinensis honey ameliorating alcohol liver damage of mice by adjusting the gut microbiota [D].Nanchang:Nanchang University,2022:4-5.
[26] 周孟焦,何鑫柱,李朝俊,等.竹叶花椒黄酮抗氧化性及协同效应研究 [J].食品与机械,2023,39(5):139-143.ZHOU M J,HE X Z,LI C J,et al.Study on antioxidant activity and synergistic effect of flavonoids from Zanthoxylum armatum DC [J].Food & Machinery,2023,39(5):139-143.
[27] TLAK GAJGER I,VLAINIĆ J.Antioxidant activity of honey bee products [J].Antioxidants,2025,14(1):64.
[28] 宋露露,李云飞,郑郭芳,等.阿胶复合风味软糖配方优化及体外模拟消化、抗氧化活性分析 [J/OL ].保鲜与加工.(2025 -04-30) [2025 -12-23].https://link.cnki.net/urlid/ 12.1330.s.20250429.1913.002.SONG L L,LI Y F,ZHENG G F,et al.Formulation optimization,in vitro simulated digestion and antioxidant activity analysis of Colla corii asini compound flavor soft candy [J/OL ].Storage and Process.(2025 -04-30) [2025 -12-23].https://link.cnki.net/urlid/ 12.1330.s.20250429.1913.002.
[29] 周佳,王丽君,禹洁,等.不同品种蜂蜜氨基酸、多酚类及生物 碱 类 化 合 物 的 主 成 分 分 析 [J].食 品 研 究 与 开 发,2024,45(24):176-186.ZHOU J,WANG L J,YU J,et al.Principal component analysis of amino acids,polyphenol compounds,and alkaloid compounds of honey from different varieties [J].Food Research and Development,2024,45(24):176-186.
[30] CHEN Y C,SHI J J,WANG X M,et al.An antioxidant feedforward cycle coordinated by linker histone variant H 1.2 and NRF 2 that drives nonsmall cell lung cancer progression [J].Proceedings of the National Academy of Sciences of the United States of America,2023,120(39):e2306288120.
[31] 韩兴,吕静,王兴妮,等.白雪茶水提物对乙醇诱导的小鼠急性酒精性肝损伤的干预作用 [J].中国食品学报,2024,24(7):172-181.HAN X,LV J,WANG X N,et al.Protective effect and mechanism of aqueous extract of thamnolia vermicularis ach on ethanol-induced acute alcoholic liver injury in mice [J].Journal of Chinese Institute of Food Science and Technology,2024,24(7):172-181.
[32] 沈 钦 海,张 盟 辉,秦 召 敏,等.槲 皮 素 预 处 理 调 节 AMPK/mTOR 信号通路介导的自噬途径改善大鼠肝缺血再灌注损伤[J].中国比较医学杂志,2022,32(3):16-22.SHEN Q,ZHANG M,QIN Z,et al.Quercetin pretreatment modulates the autophagy pathway mediated by the AMPK/m TOR signaling pathway and improves liver ischemia-reperfusion injury in rats [J].Chinese Journal of Comparative Medicine,2022,32(3):16-22.
[33] 陈瑶,向正国,郑扬.前列地尔联合双歧杆菌对急性胰腺炎患 者 的 疗 效 及 对 肠 屏 障 功 能 和 闭 合 蛋 白 水 平 的 影 响 [J].临床急诊杂志,2022,23(1):15-18,24.CHEN Y,XIANG Z,ZHENG Y.Effect of alprostadil combined with bifidobacterium on intestinal barrier function and occludin level in patients with acute pancreatitis [J].Journal of Clinical Emergency,2022,23(1):15-18,24.
[34] 林凤屏,陆佳宏,谢瑶瑶,等.蜂蜜的化学活性成分及其功能研究进展 [J].福建轻纺,2025 (7):17-22.LIN F P,LU J H,XIE Y Y,et al.Research progress on the chemical active components and functions of honey [J].The Light & Textile Industries of Fujian,2025 (7):17-22.
[35] XIAO X,CUI T T,QIN S K,et al.Beneficial effects of Lactobacillus plantarum on growth performance,immune status,antioxidant function and intestinal microbiota in broilers[J].Poultry Science,2024,103(12):104280.
[36] ZHANG F,LI D P,YANG Y W,et al.Combined effects of polystyrene microplastics and copper on antioxidant capacity,immune response and intestinal microbiota of Nile tilapia (Oreochromis niloticus ) [J].Science of the Total Environment,2022,808:152099.
[37] LIU Z Q,YAN C Z,ZHONG S M,et al.Dietary antrodia cinnamomea polysaccharide intervention modulates clinical symptoms by regulating ovarian metabolites and restructuring the intestinal microbiota in rats with letrozole-induced PCOS[J].Journal of Agricultural and Food Chemistry,2024,72(50):27 884-27 901.
[38] YANG W T,HUANG Z H,XIONG H,et al.Rice protein peptides alleviate dextran sulfate sodium-induced colitis via the Keap 1-Nrf2 signaling pathway and regulating gut microbiota [J].Journal of Agricultural and Food Chemistry,2022,70(39):12 469-12 483.