Screening and fragmentation pattern analysis of oxyphenisatine and its derivatives in dietetic foods
Abstract
[Objective] To establish a screening method for illegally added oxyphenisatine and its derivatives in dietetic foods using ultra-performance liquid chromatography (UPLC) and UPLC quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). [Methods] A UV spectral library was constructed using reference standards, and a high-resolution mass spectral database containing retention time, precursor ions, and characteristic fragment ions was established. Tandem mass spectrometry (MS/MS) was employed to elucidate characteristic fragmentation pathways of oxyphenisatine compounds and to determine their diagnostic fragment ions. The UPLC-Q-TOF-MS method was further evaluated for data processing, specificity, limit of detection (LOD), and robustness. [Results] Oxyphenisatine compounds exhibited similar spectral features, with diagnostic fragment ions at m/z 224.070 6 and m/z 196.075 7. Chlorinated derivatives produced characteristic ions at m/z 258.032 1 and m/z 230.037 2, while fluorinated derivatives yielded ions at m/z 242.061 1 and m/z 214.066 2. The LODs ranged from 0.5 to 5.0 mg/kg. In actual sample analysis, one unknown oxyphenisatine compound was detected and confirmed to be a fluorinated diacetophenbutate. [Conclusion] This method demonstrates strong anti-interference capacity, good stability, and is suitable for the rapid screening and confirmatory analysis of oxyphenisatine compounds in dietetic foods.
Publication Date
1-23-2026
First Page
43
Last Page
49
DOI
10.13652/j.spjx.1003.5788.2025.60042
Recommended Citation
Xiaoqin, LI; Wei, WANG; Ling, ZHANG; Yezhong, CAO; and Hongliu, DING
(2026)
"Screening and fragmentation pattern analysis of oxyphenisatine and its derivatives in dietetic foods,"
Food and Machinery: Vol. 42:
Iss.
1, Article 6.
DOI: 10.13652/j.spjx.1003.5788.2025.60042
Available at:
https://www.ifoodmm.cn/journal/vol42/iss1/6
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