Abstract
[Objective] To develop an efficient detection method based on the Waters TQ-XS triple quadrupole liquid chromatography-mass spectrometry (LC-MS/MS) system for identifying cases of duck meat being substituted for beef or lamb. [Methods] Using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), specific peptide ion pairs of beef, lamb, and duck were screened and validated. A qualitative and quantitative analysis method was established under multiple reaction monitoring (MRM) mode, with optimization of sample pretreatment and mass spectrometry conditions. [Results] The method showed good linearity (R2>0.99), high sensitivity, and a minimum limit of quantification of 0.5%. Detection of samples with different adulteration ratios demonstrated that the method could accurately and rapidly identify and quantify duck meat substituted for beef or lamb. Results from actual sample testing were consistent with those obtained by PCR. [Conclusion] This method demonstrates excellent performance in sample processing and sensitivity, and is suitable for the rapid identification and quantitative analysis of duck meat adulterated into beef and lamb products. It can provide effective technical support for meat quality control, market supervision, and prevention of meat adulteration.
Publication Date
1-23-2026
First Page
58
Last Page
63
DOI
10.13652/j.spjx.1003.5788.2024.80989
Recommended Citation
Feng, ZHANG; Ying, ZOU; and Xueying, SHEN
(2026)
"Identification of duck meat adulteration in beef and lamb using Waters TQ-XS Triple quadrupole liquid chromatography-mass spectrometry,"
Food and Machinery: Vol. 42:
Iss.
1, Article 8.
DOI: 10.13652/j.spjx.1003.5788.2024.80989
Available at:
https://www.ifoodmm.cn/journal/vol42/iss1/8
References
[1] SPINK J,MOYER D C.Defining the public health threat offood fraud [J].Journal of Food Science,2011,76(9):R157-R163.
[2] EVERSTINE K,SPINK J,KENNEDY S.Economically motivated adulteration (EMA ) of food:common characteristics of EMA incidents [J].Journal of Food Protection,2013,76(4):723-735.
[3] 贝君,王珂雯,程雅晴,等.我国肉制品安全风险及监管建议[J].食品安全质量检测学报,2020,11(16):5 540-5 546.BEI J,WANG K W,CHENG Y Q,et al.Safety risks and supervision suggestions of meat products in China [J].Journal of Food Safety & Quality,2020,11(16):5 540-5 546.
[4] RUBERT J,ZACHARIASOVA M,HAJSLOVA J.Advances in high-resolution mass spectrometry based on metabolomics studies for food –a review [J].Food Addit Contam Part A Chem Anal Control Expo Risk Assess,2015,32(10):1 685-1 708.
[5] 付尚辰,李玲,郑卫民,等.掺假羊乳及其制品中牛乳的检测技术研究进展 [J].食品安全质量检测学报,2021,12(8):3 000-3 007.FU S C,LI L,ZHENG W M,et al.Research progress on adulteration detection technology of cow milk in goat milk and its products [J].Journal of Food Safety & Quality,2021,12(8):3 000-3 007.
[6] 余卉茹,纪艺,彭城,等.肉类掺假检测技术研究进展 [J].生物技术进展,2022,12(2):213-221.YU H R,JI Y,PENG C,et al.Research progress on detection technology of meat adulteration [J].Current Biotechnology,2022,12(2):213-221.
[7] 路宇轩,翟睿,吴帆,等.肉类掺假检测方法及研究进展 [J].计量学报,2023,44(6):1 000-1 008.LU Y X,ZHAI R,WU F,et al.Research progress on detection technology of meat adulteration [J].Acta Metrologica Sinica,2023,44(6):1 000-1 008.
[8] CARRERA M,ABRIL A G,PAZOS M,et al.Proteins and peptides:proteomics approaches for food authentication and allergen profiling [J].Current Opinion in Food Science,2024,57:101172.
[9] 张慧艳,刘诗文,齐诗哲,等.食品组学技术在食品真伪鉴别和 溯 源 方 面 应 用 进 展 [J].食 品 安 全 质 量 检 测 学 报,2022,13(3):948-955.ZHANG H Y,LIU S W,QI S Z,et al.Application progress of food omics technology in food authenticity identification and traceability [J].Journal of Food Safety & Quality,2022,13(3):948-955.
[10] NICOLICH R S,WERNECK-BARROSO E,MARQUES M A S.Food safety evaluation:detection and confirmation of chloramphenicol in milk by high performance liquid chromatography-tandem mass spectrometry [J].Analytica Chimica Acta,2006,565(1):97-102.
[11] CUBERO-LEON E,PEÑALVER R,MAQUET A.Review on metabolomics for food authentication [J].Food Research International,2014,60:95-107.
[12] 王永瑞,柏霜,罗瑞明,等.基于电子鼻、GC-MS 结合化学计量 学 方 法 鉴 别 烤 羊 肉 掺 假 [J].食 品 科 学,2022,43(4):291-298.WANG Y R,BAI S,LUO R M,et al.Identification of adulteration of roast mutton using electronic nose and gas chromatography-mass spectrometry combined with chemometric methods [J].Food Science,2022,43(4):291-298.
[13] 沈央红,方金玉,朱军莉,等.代谢组学在食品安全与质量领域应用研究进展 [J].食品科学,2023,44(5):282-289.SHEN Y H,FANG J Y,ZHU J L,et al.Advance in the application of metabonomics in the field of food safety and quality [J].Food Science,2023,44(5):282-289.
[14] STACHNIUK A,SUMARA A,MONTOWSKA M,et al.Liquid chromatography-mass spectrometry bottom-up proteomic methods in animal species analysis of processed meat for food authentication and the detection of adulterations[J].Mass Spectrometry Reviews,2021,40(1):3-30.
[15] WINTON L M,STONE J K,WATRUD L S,et al.Simultaneous one-tube quantification of host and pathogen DNA with real-time polymerase chain reaction [J].Phytopathology,2002,92(1):112-116.
[16] PINHEIRO L B,COLEMAN V A,HINDSON C M,et al.Evaluation of a droplet digital polymerase chain reaction format for DNA copy number quantification [J].Analytical Chemistry,2012,84(2):1 003-1 011.
[17] 黎星,吴婉琴,陈冉,等.超高效液相色谱—高分辨质谱组学技 术 鉴 别 咖 啡 掺 假 [J].食 品 安 全 质 量 检 测 学 报,2023,14(24):169-176.LI X,WU W Q,CHEN R,et al.Identification of coffee adulteration by ultra performance liquid chromatography-high resolution mass spectrometry combined with omics technology[J].Journal of Food Safety & Quality,2023,14(24):169-176.
[18] 刘楠,许利丽,张晓梅,等.基于高分辨质谱的食品致敏蛋白检测研究进展 [J].分析测试学报,2023,42(12):1 666-1 672.LIU N,XU L L,ZHANG X M,et al.Research progress on detection of food allergic protein based on high-resolution mass spectrometry [J].Journal of Instrumental Analysis,2023,42(12):1 666-1 672.
[19] 魏莹,杨艳歌,赵健淞,等.食源性甲型肝炎病毒快速检测技术研究进展 [J].食品安全质量检测学报,2024,15(6):74-82.WEI Y,YANG Y G,ZHAO J S,et al.Research progress of rapid detection technology for foodborne hepatitis A virus [J].Journal of Food Safety & Quality,2024,15(6):74-82.
[20] 孟佳,钮冰,古淑青,等.基于液相色谱—质谱的蛋白质组学技术在食品鉴伪中的应用 [J].食品安全质量检测学报,2019,10(4):998-1 003.MENG J,NIU B,GU S Q,et al.Application of proteomics technology based on liquid chromatography-mass spectrometry in food identification [J].Journal of Food Safety & Quality,2019,10(4):998-1 003.
