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Corresponding Author(s)

汪超(1978—),男,湖北工业大学教授,博士。E-mail:[email protected]

Abstract

[Objective] To investigate the effects of sodium carbonate addition levels on the quality and digestibility of soybean textured protein during extrusion expansion. [Methods] Soybean protein isolate is used as the raw material, and sodium carbonate is added at mass fractions of 0,0.25%, 0.50%, and 1.00% during extrusion expansion. Subsequently, the basic physicochemical properties, color characteristics, micro - and macro -morphology, intermolecular forces, and levels of carbonyl and sulfhydryl groups of the samples from different treatment groups are characterized. Additionally, the differences in the formation of high -temperature processing hazards and in vitro digestibility among the samples are comprehensively compared. [Results] Compared with the group without alkali addition, the texturization degree and water -holding capacity of the samples with 0.5% addition increase by 34.09% and 13.41%, respectively;The bulk density decreases by 10.00%, and the color was significantly improved (P<0.05). The sulfhydryl content increases by 45.74%, the carbonyl content decreases by 21.43%, and the intermolecular interactions are adjusted, while hydrophobic interactions, disulfide bonds, and hydrogen bonds remain the main forces overall. Fourier transform infrared spectroscopy analysis further indicates that the effect of alkali addition on the secondary structure of the textured protein is significantly weaker than that of the extrusion expansion processing. Moreover, compared with the group without alkali addition, the 0.5% alkali addition treatment significantly increases the reducing sugar content in the textured protein by 25.65%, while the content of malondialdehyde, advanced glycation end products, and 5-hydroxymethylfurfural decrease by 33.08%, 9.72%, and 40.68%, respectively. In vitro digestion shows that alkali treatment could reduce the gastric digestion rate, helping to prolong satiety, with no significant effect on intestinal digestion. [Conclusion] Appropriate alkali addition during extrusion expansion can effectively optimize the textural quality and safety of soybean textured protein.

Publication Date

11-3-2026

First Page

123

Last Page

131

DOI

10.13652/j.spjx.1003.5788.2026.80296

References

[1] Zhan Y,Guo Z L,Podgorski J,et al.Changes in meat consumption can improve groundwater quality [J].Nature Food,2025,6(7):703-714.

[2] Sha L,Xiong Y L.Plant protein-based alternatives of reconstructed meat:science,technology,and challenges [J].Trends in Food Science & Technology,2020,102:51-61.

[3] Zhou H,Wang Q,Zhang J C.Extruder die innovations for the texturization of plant proteins applied in plant-based products[J].Comprehensive Reviews in Food Science and Food Safety,2026,25(1):e70367.

[4] Van Den Berg L A,Mes J J,Mensink M,et al.Protein quality of soy and the effect of processing:a quantitative review [J].Frontiers in Nutrition,2022,9:1004754.

[5] Limpisophon K,Ma X F,Sagis L M C,et al.Synergistic effects of alkaline and heat treatments on structural and functional properties of mung bean protein isolate:improving physicochemical stability of plant-based emulsions [J].International Journal of Food Science & Technology,2024,59(12):9 203-9 219.

[6] Sun P,Zhang Q,Zhao Y,et al.Improving gel properties of soy protein isolate through alkaline pH-shifting,mild heat treatment,and TGase cross-linking [J].Food Hydrocolloids,2023,144:108924.

[7] Li X,Manickavasagan A,Lim L T.Reduction of antinutrients and off-flavour in kidney bean flour by acidic and alkaline reactive extrusion [J].Food Research International,2024,192:114832.

[8] Zhang F Y,Yang B,Zhang M,et al.Influence of sodium bicarbonate and moisture content on physicochemical properties of chicken-based extrudate by twin-screw extruder [J].Food Chemistry,2023,402:134313.

[9] Martin D,Harnisch L,Leister N,et al.Network formation in pea protein-based meat substitutes:effects of pH,oil,and pectin-based microgel particles [J].Food Research International,2026,242:120006.

[10] Li T,Guo X N,Zhu K X,et al.Effects of alkali on protein polymerization and textural characteristics of textured wheat protein [J].Food Chemistry,2018,239:579-587.

[11] He C X,Zhou W B.Conformational engineering and functional enhancement of pea protein via a two-step Na2CO3-acidification process [J].Food Hydrocolloids,2026,172:111935.

[12] Ma Y Y,Chen Y,Chen F S,et al.Effects of pH-shifting combined with ion immersion on gel properties and protein aggregation behavior of soybean protein isolate [J].Food Hydrocolloids,2025,158:110562.

[13] Li J H,Zhang W J,Tang T T,et al.Thermal gelation and digestion properties of hen egg white:study on the effect of neutral and alkaline salts addition [J].Food Chemistry,2023,409:135263.

[14] 余杰,贾利蓉,张贞炜,等.复合蛋白原料制备高水分拉丝蛋白的工艺优化研究 [J].中国粮油学报,2024,39(11):124-130.Yu J,Jia L R,Zhang Z W,et al.Optimization of process for preparation of high moisture extruded proteins from composite protein raw materials [J].Journal of the Chinese Cereals and Oils Association,2024,39(11):124-130.

[15] Qi L Y,Wang M L,Li Y Q,et al.Evolution of fibrous structure and physicochemical properties of pea protein matrices during high moisture extrusion:Impacts of zein and extrusion zone [J].Food Hydrocolloids,2025,166:111379.

[16] 吕培楷,刘晓媛,徐丹鹤,等.板栗蛋白和石榴多酚复配对鳙鱼鱼糜凝胶品质的影响 [J].食品工业科技,2026,47(3):62-69.Lü P K,Liu X Y,Xu D H,et al.Effects of chestnut protein and pomegranate polyphenols on gel quality of bighead surimi [J].Science and Technology of Food Industry,2026,47(3):62-69.

[17] Zhang M,Hou Y Q,Xia S Q,et al.Modulating the aggregation pattern of interface myofibrillar protein in emulsion gel to enhance Na+ inhomogeneous distribution during microwave heating:the effects of hydrophobic interactions and disulfide bonding [J].Food Research International,2025,217:116762.

[18] Song J Q,Yang Z,Zeng J K,et al.Aggregation mechanism of soybean protein isolate for the formation of plant protein-based meat analogs during high moisture extrusion [J].International Journal of Biological Macromolecules,2026,337:149524.

[19] 李越佳,王琛,李潇,等.不同加热条件对梅花鹿腿肉品质及其肌原纤维蛋白特性的影响 [J].食品与发酵工业,2026,52(10):258-266.Li Y J,Wang C,Li X,et al.Effects of different heating conditions on meat quality and myofibrillar protein properties of Sika deer leg meat [J].Food and Fermentation Industries,2026,52(10):258-266.

[20] 王紫鑫,谭苗苗,陈敬鑫,等.γ-谷维素 +β-谷甾醇基凝胶油对金线鱼鱼糜凝胶和体外消化特性的影响 [J].食品工业科技,2026,47(18):105-114.Wang Z X,Tan M M,Chen J X,et al.Effect of γ-oryzanol+β-sitosterol-based oleogels on the gel and digestive properties of Nemipterus virgatus surimi [J].Science and Technology of Food Industry,2026,47(18):105-114.

[21] Zhang S,Sheng Y N,Feng Y C,et al.Changes in structural and functional properties of globulin-polyphenol complexes in |mung beans:exploration under different interaction ratios and heat treatment conditions [J].International Journal of Food Science & Technology,2022,57(4):1 920-1 935.

[22] 崔国庭,王缎,郭金英,等.加工方式对面筋蛋白结构及性质影响的研究进展 [J].食品与机械,2024,40(3):225-232.Cui G T,Wang D,Guo J Y,et al.Research progress on the effects of processing methods on gluten protein structure and properties [J].Food & Machinery,2024,40(3):225-232.

[23] Ozturk O K,Salgado A M,Holding D R,et al.Dispersion of zein into pea protein with alkaline agents imparts cohesive and viscoelastic properties for plant-based food analogues [J].Food Hydrocolloids,2023,134:108044.

[24] 张百汝,王斌,王琨,等.挤压处理对豌豆蛋白功能特性及结构的影响 [J].食品与机械,2022,38(1):32-37.Zhang B R,Wang B,Wang K,et al.Effect of extrusion treatment on the functional properties and structure of pea protein [J].Food & Machinery,2022,38(1):32-37.

[25] Rolandelli G,Eeckhout M.Molecular interactions of biopolymers during high-moisture extrusion and their impacts on micro- and macrostructural properties of plant-based meat analogues:a review [J].Trends in Food Science & Technology,2026,168:105507.

[26] Wu X Y,Zhang M H,Adhikari B,et al.Synergistic effects of ultrasonication and alkaline treatment on curcumin-induced cross-linking and gelation of casein-soy protein isolate hybrids[J].Ultrasonics Sonochemistry,2025,123:107690.

[27] Patel J D,Gao Z L,He L L.FTIR spectroscopic analysis of plant proteins and correlation with functional properties [J].Journal of AOAC International,2025,108(3):348-356.

[28] 李岩,曾祥权,杜文斌,等.不同植物油对植物肉风味及感官特性的影响 [J].食品科学技术学报,2022,40(2):31-44.Li Y,Zeng X Q,Du W B,et al.Effect of different vegetable oils on flavor and sensory characteristics of plant-based meat[J].Journal of Food Science and Technology,2022,40(2):31-44.

[29] Bleicher J,Ebner E E,Bak K H.Formation and analysis of volatile and odor compounds in meat:a review [J].Molecules,2022,27(19):6 703.

[30] 高春慧,苏玉,刘时瑜,等.板栗加工过程中褐变控制工艺优化及指标分析 [J].食品与机械,2025,41(1):225-233.Gao C H,Su Y,Liu S Y,et al.Optimization of browning control process in chestnut processing and analysis of its indicators [J].Food & Machinery,2025,41(1):225-233.

[31] Yu L G,Zhang X Y,Sun W Y,et al.The influence of piperine on oxidation-induced porcine myofibrillar protein gelation behavior and fluorescent advanced glycation end products formation in model systems [J].Food Chemistry,2023,420:136119.

[32] Osiecka D,Vakh C,Makoś -Chełstowska P,et al.Plant-based meat substitute analysis using microextraction with deep eutectic solvent followed by LC-MS/MS to determine acrylamide,5-hydroxymethylfurfural and furaneol [J].Analytical and Bioanalytical Chemistry,2024,416(5):1 117-1 126.

[33] Gong X X,Sun Q C,Wang X Y,et al.Recent advances in pulse protein conjugation and complexation with polyphenols:an emerging approach to improve protein functionality and health benefits [J].Critical Reviews in Food Science and Nutrition,2025,65(7):1 279-1 289.

[34] Yang S,Lian Z T,Wang M M,et al.Molecular structural modification of β-conglycinin using pH-shifting with ultrasound to improve emulsifying properties and stability [J].Ultrasonics Sonochemistry,2022,90:106186.

[35] 成晓苑,马成业,李宏军.挤压预处理对黑豆粕蛋白质消化率和酱油品质、抗氧化活性的影响 [J].食品与机械,2026,42(8):205-212.Cheng X Y,Ma C Y,Li H J.Extrusion pretreatment improves the protein digestibility of black soybean meal and its effects on the final quality and antioxidant activity of soy sauce [J].Food & Machinery,2026,42(8):205-212.

[36] Zhou H L,Hu Y Y,Tan Y B,et al.Digestibility and gastrointestinal fate of meat versus plant-based meat analogs:an in vitro comparison [J].Food Chemistry,2021,364:130439.

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