Abstract
[Objective] To investigate the in vivo anti-aging effects of the aqueous extract of Dendrobium officinale leaves and its potential mechanisms. [Methods] The contents of the main bioactive components, flavonoids and polysaccharides, in the aqueous extract of D.officinale leaves were determined. Using Drosophila melanogaster as a model, the effects of the aqueous extract on the lifespan, locomotor capacity, heat stress tolerance, and tolerance to H2O2-induced oxidative stress in D.melanogaster were evaluated, and the activities of total superoxide dismutase (T-SOD ), cytosolic Cu/Zn -superoxide dismutase 1 (SOD 1), mitochondrial Mn -superoxide dismutase 2 (SOD 2), and catalase (CAT ) were measured. [Results] The contents of flavonoids and polysaccharides in the aqueous extract of D.officinale leaves were 0.634% and 11.118%, respectively. Compared with the control group, the median lethal time, mean lifespan, and maximum lifespan of D.melanogaster in the medium -dose group were prolonged by 13.67%, 13.93%, and 10.81%, respectively;Locomotor capacity was significantly improved, and tolerance to H2O2-induced acute oxidative stress was significantly enhanced, whereas heat stress tolerance was reduced. The activities of T -SOD, SOD 1, and SOD 2 increased by 27.80%, 34.09%, and 19.57%, respectively, while CAT activity showed no significant change. [Conclusion] The aqueous extract of D.officinale leaves exerts anti -aging effects by enhancing antioxidant enzyme activities and improving resistance to oxidative stress.
Publication Date
11-3-2026
First Page
132
Last Page
138
DOI
10.13652/j.spjx.1003.5788.2026.80663
Recommended Citation
Shuting, Li; Lin, Yang; and Yan, Huang
(2026)
"Evaluation of the anti-aging effects of aqueous extract from Dendrobium officinale leaves on Drosophila melanogaster,"
Food and Machinery: Vol. 42:
Iss.
10, Article 15.
DOI: 10.13652/j.spjx.1003.5788.2026.80663
Available at:
https://www.ifoodmm.cn/journal/vol42/iss10/15
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